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Comparative Study of Monoclonal and Recombinant Antibody-Based Immunoassays for Fungicide Analysis in Fruit juices

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Comparative Study of Monoclonal and Recombinant Antibody-Based Immunoassays for Fungicide Analysis in Fruit juices

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dc.contributor.author Moreno Tamarit, Mª José es_ES
dc.contributor.author Plana Andani, Emma es_ES
dc.contributor.author Manclus Ciscar, Juan José es_ES
dc.contributor.author Montoya Baides, Ángel es_ES
dc.date.accessioned 2014-10-13T06:46:36Z
dc.date.available 2014-10-13T06:46:36Z
dc.date.issued 2014-02
dc.identifier.issn 1936-9751
dc.identifier.uri http://hdl.handle.net/10251/43166
dc.description.abstract [EN] A comparative study of the analytical performance of enzyme-linked immunosorbent assays (ELISAs), based on monoclonal and recombinant antibodies, for the determination of fungicide residues in fruit juices has been carried out. To this aim, three murine hybridoma cell lines secreting specific monoclonal antibodies against (RS)-2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazol-1-yl)propyl-1,1,2,2-tetrafluoroethyl ether (tetraconazole), 2-(4-triazolyl)benzimidazole (thiabendazole), and (RS)-1-(beta-allyloxy-2,4-dichlorophenylethyl)imidazole (imazalil) were used as a source of immunoglobulin gene fragments for the production of single-chain variable fragment (scFv) and fusion scFv-pIII recombinant antibodies in Escherichia coli. Selected recombinant antibodies displayed cross-reactivity profiles very similar to those of the parent monoclonal antibodies. Imazalil and tetraconazole recombinant antibodies showed one order of magnitude lower affinity than their respective monoclonal antibodies, whereas the thiabendazole recombinant antibodies showed an affinity similar to that of their parent monoclonal antibody. On the other hand, scFv-pIII fusion fragments showed similar analytical properties as, and occasionally better than, scFv recombinant antibodies. Finally, ELISAs developed from each antibody type showed similar analytical performance when applied to the analysis of the target fungicides in fruit juices. es_ES
dc.description.sponsorship This work was funded by Ministerio de Educacion y Ciencia (MEC, Spain, Project AGL2002-03266). E. P. was the recipient of a doctoral fellowship from Conselleria d'Educacio (Generalitat Valenciana, Spain). en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Food Analytical Methods es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Fungicide residues es_ES
dc.subject Monoclonal antibodies es_ES
dc.subject Recombinant antibodies es_ES
dc.subject Immunoanalysis es_ES
dc.subject ELISA es_ES
dc.subject Fruit juices es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Comparative Study of Monoclonal and Recombinant Antibody-Based Immunoassays for Fungicide Analysis in Fruit juices es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s12161-013-9655-z
dc.relation.projectID info:eu-repo/grantAgreement/MICYT//AGL2002-03266/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.description.bibliographicCitation Moreno Tamarit, MJ.; Plana Andani, E.; Manclus Ciscar, JJ.; Montoya Baides, Á. (2014). Comparative Study of Monoclonal and Recombinant Antibody-Based Immunoassays for Fungicide Analysis in Fruit juices. Food Analytical Methods. 7(2):481-489. https://doi.org/10.1007/s12161-013-9655-z es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s12161-013-9655-z es_ES
dc.description.upvformatpinicio 481 es_ES
dc.description.upvformatpfin 489 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 264009
dc.contributor.funder Ministerio de Ciencia y Tecnología es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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