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Giant unilamellar vesicles investigated by optical stretching, fluorescence microscopy and image analysis

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Giant unilamellar vesicles investigated by optical stretching, fluorescence microscopy and image analysis

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dc.contributor.advisor Berg Sorensen, Kirstine es_ES
dc.contributor.author Guiñón Catalán, Héctor es_ES
dc.date.accessioned 2014-11-04T11:45:37Z
dc.date.available 2014-11-04T11:45:37Z
dc.date.created 2014-03
dc.date.issued 2014-11-04T11:45:37Z
dc.identifier.uri http://hdl.handle.net/10251/43842
dc.description.abstract Consulta en la Biblioteca ETSI Industriales (Riunet) es_ES
dc.description.abstract [es] In this project giant unilamellar vesicles made of di erent phospholipids are produced by electroformation and rehydration methods. The vesicles contained sucrose inside and were suspended in a glucose solution. The vesicle average size obtained is between 10 and 60 micrometers, although a wide dispersion was found, from 300 nm up to 1000 micrometers. The Young's modulus of the vesicle membranes is obtained by optical stretching. For the stretcher, several methods are tested, especially focusing on improving and simplifying the set up and the procedures. In the chip that showed better results, optical bres are inserted in syringe needles that protects them when handling, and needles are placed in a socket built on a microscope glass slide. Vesicles are stretched with two lasers with a maximum power of 300 mW, exerting a maximum force at the membrane surface of 20 pN. Deformations are determined by image analysis with Matlab. Several image tools are implemented, noise-removing and image enhancement for identifying data, and extraction of image characteristics to obtain the measurements. The software is implemented in a graphic interface to allow to control the parameters manually. For a vesicle composition 75% DOPC 25% Asolectin and a diameter of 18.36 0.41 m the Young's modulus was determined from an elastic shell model as Y = 3.35 1.23 Pa. es_ES
dc.language Inglés es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Consulta en la Biblioteca ETSI Industriales es_ES
dc.subject Vesículas unilamelares es_ES
dc.subject Análisis de imágen es_ES
dc.subject.classification INGENIERIA DE SISTEMAS Y AUTOMATICA es_ES
dc.subject.other Ingeniero Industrial-Enginyer Industrial es_ES
dc.title Giant unilamellar vesicles investigated by optical stretching, fluorescence microscopy and image analysis es_ES
dc.type Proyecto/Trabajo fin de carrera/grado es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.description.bibliographicCitation Guiñón Catalán, H. (2014). Giant unilamellar vesicles investigated by optical stretching, fluorescence microscopy and image analysis. http://hdl.handle.net/10251/43842. es_ES
dc.description.accrualMethod Archivo delegado es_ES


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