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Biomolecular conjugation inside synthetic polymer nanopores via glycoprotein-lectin interactions

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Biomolecular conjugation inside synthetic polymer nanopores via glycoprotein-lectin interactions

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dc.contributor.author Mubarak, Ali es_ES
dc.contributor.author Ramirez Hoyos, Patricio es_ES
dc.contributor.author Tahir, Muhammad Nawaz es_ES
dc.contributor.author Mafé, Salvador es_ES
dc.contributor.author Siwy, Zuzanna S. es_ES
dc.contributor.author Neumann, Reinhard es_ES
dc.contributor.author Tremel, W. es_ES
dc.contributor.author Ensinger, Wolfgang J. es_ES
dc.date.accessioned 2014-11-24T09:54:58Z
dc.date.available 2014-11-24T09:54:58Z
dc.date.issued 2011-03-18
dc.identifier.issn 2040-3364
dc.identifier.uri http://hdl.handle.net/10251/44599
dc.description.abstract We demonstrate the supramolecular bioconjugation of concanavalin A (Con A) protein with glycoenzyme horseradish peroxidase (HRP) inside single nanopores, fabricated in heavy ion tracked polymer membranes. Firstly, the HRP-enzyme was covalently immobilized on the inner wall of the pores using carbodiimide coupling chemistry. The immobilized HRP-enzyme molecules bear sugar (mannose) groups available for the binding of Con A protein. Secondly, the bioconjugation of Con A on the pore wall was achieved through its biospecific interactions with the mannose residues of the HRP enzyme. The immobilization of biomolecules inside the nanopore leads to the reduction of the available area for ionic transport, and this blocking effect can be exploited to tune the conductance and selectivity of the nanopore in aqueous solution. Both cylindrical and conical nanopores were used in the experiments. The possibility of obtaining two or more conductance states (output), dictated by the degree of nanopore blocking resulted from the different biomolecules in solution (input), as well as the current rectification properties obtained with the conical nanopore, could also allow implementing information processing at the nanometre scale. Model simulations based on the transport equations further verify the feasibility of the sensing procedure that involves concepts from supramolecular chemistry, molecular imprinting, recognition, and nanotechnology. © 2011 The Royal Society of Chemistry. es_ES
dc.language Español es_ES
dc.publisher Royal Society of Chemistry es_ES
dc.relation.ispartof Nanoscale es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Aqueous solutions es_ES
dc.subject Bio-conjugation es_ES
dc.subject Bio-molecular es_ES
dc.subject Biospecific interaction es_ES
dc.subject Blocking effect es_ES
dc.subject Carbodiimide-coupling chemistry es_ES
dc.subject Concanavalin A es_ES
dc.subject Conductance state es_ES
dc.subject Conical nanopores es_ES
dc.subject Enzyme molecules es_ES
dc.subject Horseradish peroxidase es_ES
dc.subject Information processing es_ES
dc.subject Inner walls es_ES
dc.subject Ionic transports es_ES
dc.subject Model simulation es_ES
dc.subject Molecular imprinting es_ES
dc.subject Nanometres es_ES
dc.subject Polymer membrane es_ES
dc.subject Pore wall es_ES
dc.subject Rectification properties es_ES
dc.subject Synthetic polymers es_ES
dc.subject Transport equation es_ES
dc.subject Biomolecules es_ES
dc.subject Computer simulation es_ES
dc.subject Cylinders (shapes) es_ES
dc.subject Data processing es_ES
dc.subject Electric rectifiers es_ES
dc.subject Enzymes es_ES
dc.subject Functional polymers es_ES
dc.subject Sugars es_ES
dc.subject Supramolecular chemistry es_ES
dc.subject Nanopores es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Biomolecular conjugation inside synthetic polymer nanopores via glycoprotein-lectin interactions es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1039/C1NR00003A
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Mubarak, A.; Ramirez Hoyos, P.; Tahir, MN.; Mafé, S.; Siwy, ZS.; Neumann, R.; Tremel, W.... (2011). Biomolecular conjugation inside synthetic polymer nanopores via glycoprotein-lectin interactions. Nanoscale. 3:1894-1903. https://doi.org/10.1039/C1NR00003A es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1039/C1NR00003A es_ES
dc.description.upvformatpinicio 1894 es_ES
dc.description.upvformatpfin 1903 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 3 es_ES
dc.relation.senia 215173
dc.identifier.eissn 2040-3372


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