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Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

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Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

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dc.contributor.author Vallés Lluch, Ana es_ES
dc.contributor.author Arnal Pastor, María Pilar es_ES
dc.contributor.author Martínez Ramos, Cristina es_ES
dc.contributor.author Vilariño Feltrer, Guillermo es_ES
dc.contributor.author Vikingsson, Line es_ES
dc.contributor.author Castells Sala, Carla es_ES
dc.contributor.author Semino, Carlos Eduardo es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.date.accessioned 2015-03-02T12:02:43Z
dc.date.available 2015-03-02T12:02:43Z
dc.date.issued 2013-12
dc.identifier.issn 1742-7061
dc.identifier.uri http://hdl.handle.net/10251/47608
dc.description.abstract [EN] Some of the problems raised by the combination of porous scaffolds and self-assembling peptide (SAP) gels as constructs for tissue engineering applications are addressed for the first time. Scaffolds of poly(- ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physicochemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold’s pores was assessed, and the kinetics of its release in phosphate-buffered saline was followed. Cell seeding and colonization of these constructs were preliminarily studied with L929 fibroblasts and subsequently checked with sheep adipose-tissue-derived stem cells intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffold-cum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the three-dimensional (3-D) structure. These constructs could be of use in different advanced tissue engineering applications, where, apart from a cell-friendly extracellular matrix -like aqueous environment, a larger-scale 3-D structure able to keep the cells in a specific place, give mechanical support and/or conduct spatially the tissue growth could be required. es_ES
dc.description.sponsorship The authors acknowledge funding through the European Commission FP7 project RECATABI (NMP3-SL-2009-229239), and from the Spanish Ministerio de Ciencia e Innovacion through projects MAT2011-28791-C03-02 and -03. Dr. J.C. Chachques (Hopital Europeen Georges Pompidou, Paris) is thanked for providing the ASCs employed in this study. MMP acknowledges support of CIBER-BBN initiative, financed by Institut de Salud Carlos III (Spain) with the assistance of the European Regional Development Fund. en_EN
dc.language Español es_ES
dc.publisher Elsevier es_ES
dc.relation MICINN/MAT2011-28791-C03-02 es_ES
dc.relation MICINN/MAT2011-28791-C03-03 es_ES
dc.relation.ispartof Acta Biomaterialia es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Scaffold es_ES
dc.subject Poly(ethyl acrylate) es_ES
dc.subject Self-assembling peptide gel es_ES
dc.subject Controlled release es_ES
dc.subject Cell seeding es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.title Combining self-assembling peptide gels with three-dimensional elastomer scaffolds es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.actbio.2013.07.038
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/229239/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Vallés Lluch, A.; Arnal Pastor, MP.; Martínez Ramos, C.; Vilariño Feltrer, G.; Vikingsson, L.; Castells Sala, C.; Semino, CE.... (2013). Combining self-assembling peptide gels with three-dimensional elastomer scaffolds. Acta Biomaterialia. 9(12):9451-9460. doi:10.1016/j.actbio.2013.07.038 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.actbio.2013.07.038 es_ES
dc.description.upvformatpinicio 9451 es_ES
dc.description.upvformatpfin 9460 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 12 es_ES
dc.relation.senia 253419
dc.identifier.eissn 1878-7568
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Ciencia e Innovación (MICINN)
dc.contributor.funder Instituto de Salud Carlos III (ISCIII)


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