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Effect of thermal regime on the expression of key reproductive genes during hormonally-induced vitellogenesis in female European eels

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Effect of thermal regime on the expression of key reproductive genes during hormonally-induced vitellogenesis in female European eels

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dc.contributor.advisor Asturiano Nemesio, Juan Francisco es_ES
dc.contributor.advisor Sánchez Peñaranda, David es_ES
dc.contributor.author Mazzeo, Ilaria es_ES
dc.date.accessioned 2015-03-30T12:06:04Z
dc.date.available 2015-03-30T12:06:04Z
dc.date.created 2014-12-19 es_ES
dc.date.issued 2015-12-19 es_ES
dc.identifier.uri http://hdl.handle.net/10251/48490
dc.description.abstract European eel (Anguilla anguilla, L., 1758) is suffering a strong population decrease and at the same time it is a very appreciated species and by now it has not been possible closing its cycle life. In fact, this species does not mature in captivity unless hormonally induced. So all the production is up to the natural population. All these factors together make urgent achieving the closing of the productive cycle and for this aim it is important to understand the reproductive physiology and the reasons of this development blockage. The present thesis wants to be a new contribution to the knowledge of reproductive physiology in female European eel submitted at hormonal treatment. To achieve this goal, expression of genes not previously studied in this species (cyp19a1, ara, arb, gnrhr1a, gnrhr1b, gnrhr2, zpb and zpc) was analyzed in eels reared under a constant thermal regime, accordingly to the usual rearing conditions. Also, the effect of rearing temperature on gene expression and steroid profile (T, 11-KT and E2) was studied. In fact, eels migrate to Sargasso Sea to reproduce and during the travel experiment temperature changes, while traditionally they are reared at a constant high temperature which could affect vitellogenesis progression and final oocyte quality. For the study it was necessary cloning and characterizing some genes which have not still been sequenced in European eel. Gene expression was studied by qPCR after designing primer and optimizing the qPCR race. Steroid profiles were analyzed by immunoassays and the gonadal development stages were established by histology. The first result obtained at the end of the study were six new genes characterized in European eel. The analysis of gene expression allowed to understand the involvement of specific genes during vitellogenesis (arb, gnrhr1b and gnrhr2) in different brain regions. The temperature was conformed as a crucial environmental factor affecting vitellogenesis. On one hand, eels matured at lower starting temperatures showed better reproductive parameters which could have an influence in the final oocyte quality. On the other hand higher temperatures are necessary to achieve further vitellogenetic stages en_EN
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject European eel es_ES
dc.subject Reproductive physiology: GnRH receptors es_ES
dc.subject Androgen receptors es_ES
dc.subject Aromatase es_ES
dc.subject Zona Pellucida es_ES
dc.subject Gene characterization es_ES
dc.subject QPCR es_ES
dc.subject Steroids es_ES
dc.subject Vitellogenesis es_ES
dc.subject Temperature effect. es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Effect of thermal regime on the expression of key reproductive genes during hormonally-induced vitellogenesis in female European eels es_ES
dc.type Tesis doctoral es_ES
dc.identifier.doi 10.4995/Thesis/10251/48490 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Mazzeo, I. (2014). Effect of thermal regime on the expression of key reproductive genes during hormonally-induced vitellogenesis in female European eels [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/48490 es_ES
dc.description.accrualMethod TESIS es_ES
dc.type.version info:eu-repo/semantics/acceptedVersion es_ES
dc.relation.pasarela TESIS\8302 es_ES


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