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Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants

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Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants

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dc.contributor.advisor Gómez Ribelles, José Luís es_ES
dc.contributor.advisor Lebourg, Myriam Madeleine es_ES
dc.contributor.author Ródenas Rochina, Joaquín es_ES
dc.date.accessioned 2015-03-31T06:02:01Z
dc.date.available 2015-03-31T06:02:01Z
dc.date.created 2015-01-13 es_ES
dc.date.issued 2015-03-31 es_ES
dc.identifier.uri http://hdl.handle.net/10251/48526
dc.description.abstract This thesis is focused on the development and evaluation of different hybrid scaffolds for the treatment of injuries in cartilage or bone. These hybrid materials were three-dimensional polycaprolactone macroporous scaffolds obtained through freeze extraction and particle leaching combined method and modified with hyaluronic acid or mineral particles. In order to facilitate the description of the obtained results, the thesis is divided in two sections dedicated to bone and cartilage tissue engineering respectively. In the case of bone tissue engineering we addressed the treatment of disorders associated with the spine that require spinal immobilization. This Thesis proposes the development of a synthetic macroporous support for intervertebral fusion as an alternative to commercial bone substitutes. Macroporous scaffolds were developed with bare polycaprolactone or its blends with polylactic acid in order to increase its mechanical properties and degradation rate. Furthermore, the scaffolds obtained were reinforced with hydroxyapatite or Bioglass®45S5 to improve their mechanical properties and turn them in bioactive scaffolds. The supports were characterized physicochemically and biologically to determine if they met the requirements of the project. Finally, materials were tested in vivo in a bone critical size defect preformed in a rabbit model against a commercial support. Cartilage engineering has been extensively studied in the last years due to the inherent limited self repair ability of this tissue. The second part of the thesis was focused in developing a construct composed by in vitro differentiated chondrocyte like cells in a hybrid scaffold for cartilage tissue engineering. Polycaprolactone hybrid substrates coated with hyaluronic acid scaffold were developed obtaining a substrate with positive influence over the development of chondrocyte phenotype in culture and able to protect the cells from excessive mechanical loading in the joint. Cell-scaffolds constructs were obtained combining hybrid scaffolds with mesenchymal stem cells and differentiating them to chondrocytes using chondrogenic culture medium combined with hypoxia, mechanical stimulus or co-culture. Finally the cellularized scaffolds were mechanically, biochemically and histologically characterized to determine the production of extracellular matrix and expression of chondrogenic markers. en_EN
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Tissue engineering es_ES
dc.subject Bone
dc.subject Cartilage
dc.subject Composite scaffolds
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants es_ES
dc.type Tesis doctoral es_ES
dc.identifier.doi 10.4995/Thesis/10251/48526 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Ródenas Rochina, J. (2015). Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/48526 es_ES
dc.description.accrualMethod TESIS es_ES
dc.type.version info:eu-repo/semantics/acceptedVersion es_ES
dc.relation.pasarela TESIS\4293 es_ES


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