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A thermodynamic switch modulates abscisic acid receptor sensitivity

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A thermodynamic switch modulates abscisic acid receptor sensitivity

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dc.contributor.author Dupeux, Florine es_ES
dc.contributor.author Santiago Cuéllar, Julia es_ES
dc.contributor.author Betz, Katja es_ES
dc.contributor.author Twycross, Jamie es_ES
dc.contributor.author Park, Sangyoung es_ES
dc.contributor.author Rodriguez, Lesia es_ES
dc.contributor.author González Guzmán, Miguel es_ES
dc.contributor.author Jensen, Malene Ringkjobing es_ES
dc.contributor.author Krasnogor, Natalio es_ES
dc.contributor.author Blackledge, Martin es_ES
dc.contributor.author Holdsworth, Michael es_ES
dc.contributor.author Cutler, Sean R. es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.contributor.author Marquez, Jose Antonio es_ES
dc.date.accessioned 2015-05-20T12:02:21Z
dc.date.issued 2011-10-19
dc.identifier.issn 0261-4189
dc.identifier.uri http://hdl.handle.net/10251/50560
dc.description.abstract [EN] Abscisic acid (ABA) is a key hormone regulating plant growth, development and the response to biotic and abiotic stress. ABA binding to pyrabactin resistance (PYR)/ PYR1-like (PYL)/Regulatory Component of Abscisic acid Receptor (RCAR) intracellular receptors promotes the formation of stable complexes with certain protein phosphatases type 2C (PP2Cs), leading to the activation of ABA signalling. The PYR/PYL/RCAR family contains 14 genes in Arabidopsis and is currently the largest plant hormone receptor family known; however, it is unclear what functional differentiation exists among receptors. Here, we identify two distinct classes of receptors, dimeric and monomeric, with different intrinsic affinities for ABA and whose differential properties are determined by the oligomeric state of their apo forms. Moreover, we find a residue in PYR1, H60, that is variable between family members and plays a key role in determining oligomeric state. In silico modelling of the ABA activation pathway reveals that monomeric receptors have a competitive advantage for binding to ABA and PP2Cs. This work illustrates how receptor oligomerization can modulate hormonal responses and more generally, the sensitivity of a ligand-dependent signalling system. es_ES
dc.description.sponsorship We are grateful to the European Synchrotron Radiation Facility (ESRF) and the EMBL for access to macromolecular crystallography beam lines. This work was supported by Ministerio de Educacion y Ciencia, Fondo Europeo de Desarrollo Regional, Consejo Superior de Investigaciones Cientificas (grant BIO2008-00221 to PLR; fellowships to JS and LR; Juan de la Cierva contract to MGG) and by the BBSRC/EPSRC grant BB/D019613/1 to CPIB (MH, JT and NK). Access to the High Throughput Crystallization facility of the Partnership for Structural Biology in Grenoble (PSB) (https://embl.fr/htxlab) was supported by the P-CUBE project funded by the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no 227764. en_EN
dc.language Inglés es_ES
dc.publisher EMBO Press es_ES
dc.relation.ispartof EMBO Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Signal transduction es_ES
dc.subject Hormone receptor es_ES
dc.subject Protein phosphatase 2C es_ES
dc.subject PYR-PYL-RCAR es_ES
dc.subject START domain es_ES
dc.subject Stress response es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title A thermodynamic switch modulates abscisic acid receptor sensitivity es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1038/emboj.2011.294
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2008-00221/ES/REGULACION POR ACIDO ABSCISICO DE LA RESPUESTA AL ESTRES HIDRICO, CRECIMIENTO Y DESARROLLO VEGETAL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/227764/EU/Infrastructure for Protein Production Platforms/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/RCUK/BBSRC/BB/D019613/1/GB/
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//BB%2FD019613%2F1/GB/Centre for Plant Integrative Biology/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Dupeux, F.; Santiago Cuéllar, J.; Betz, K.; Twycross, J.; Park, S.; Rodriguez, L.; González Guzmán, M.... (2011). A thermodynamic switch modulates abscisic acid receptor sensitivity. EMBO Journal. 30:4171-4184. https://doi.org/10.1038/emboj.2011.294 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1038/emboj.2011.294 es_ES
dc.description.upvformatpinicio 4171 es_ES
dc.description.upvformatpfin 4184 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.relation.senia 219105
dc.identifier.eissn 1460-2075
dc.identifier.pmid 21847091 en_EN
dc.identifier.pmcid PMC3199383 en_EN
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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