Jiménez Trigos, ME.; Vicente Antón, JS.; Mocé Cervera, ET.; Naturil Alfonso, C.; Fernández González, R.; Gutierrez Adan, A.; Marco Jiménez, F. (2013). Treatment with cholesterol-loaded methy-ß-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes. Cryobiology. 67(1):106-108. https://doi.org/10.1016/j.cryobiol.2013.05.006
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/51055
Title:
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Treatment with cholesterol-loaded methy-ß-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes
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Author:
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Jiménez Trigos, María Estrella
Vicente Antón, José Salvador
Mocé Cervera, Eva Teresa
Naturil Alfonso, Carmen
Fernández González, R.
Gutierrez Adan, Alfonso
Marco Jiménez, Francisco
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UPV Unit:
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Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
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Issued date:
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Abstract:
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Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase ...[+]
Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol has been added to the plasma membrane. This study was conducted to determine if cholesterol could be incorporated into rabbit oocytes by incubation with cholesterol-loaded methyl-β-cyclodextrin (CLC) and if added cholesterol could improve the developmental ability of cryopreserved oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fresh, frozen and vitrified oocytes incubated with CLC containing 20% NBD-labelled cholesterol (NBD-CLC) were evaluated using confocal microscopy. Fluorescence intensity was higher in fresh oocytes than in cryopreserved ones. Pre-treating rabbit oocytes with 1mg of NBD-CLC/mL did not improve cleavage and developmental rates after cryopreservation. Results showed that treatment with CLC increased the cytoplasmic cholesterol content, but did not improve cleavage rate and developmental competence of cryopreserved oocytes.
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Subjects:
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Cholesterol
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Cyclodextrin
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Vitrification
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Cryotop
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Slow-freezing
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Copyrigths:
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Cerrado |
Source:
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Cryobiology. (issn:
0011-2240
)
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DOI:
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10.1016/j.cryobiol.2013.05.006
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Publisher:
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Elsevier
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Publisher version:
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http://dx.doi.org/10.1016/j.cryobiol.2013.05.006
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Project ID:
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info:eu-repo/grantAgreement/MICINN//AGL2011-30170-C02-02/ES/ESTUDIO DEL SISTEMA INMUNE DE DIFERENTES LINEAS GENETICAS CUNICOLAS Y SU RESPUESTA FRENTE A DESAFIOS PRODUCTIVOS E INFECCIOSOS/
info:eu-repo/grantAgreement/GVA//PROMETEO09%2F2009%2F125/ES/Efecto de la crio conservación de embriones sobre el desarrollo y el restablecimiento de poblaciones/
info:eu-repo/grantAgreement/GVA//ACIF%2F2010%2F262/
info:eu-repo/grantAgreement/MICINN//RYC-2010-06162/ES/RYC-2010-06162/
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Thanks:
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This work was supported by the Spanish Research Project AGL2011-30170-C02-01 and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella Jimenez was supported by a research Grant from the ...[+]
This work was supported by the Spanish Research Project AGL2011-30170-C02-01 and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella Jimenez was supported by a research Grant from the Education Ministry of the Valencian Regional Government (programme VALi+d. ACIF/2010/262). Eva Moce was supported by funds from the Ministry of Science and Innovation Ramon y Cajal sub-programme (Ref. RYC-2010-06162; Madrid, Spain).
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Type:
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Artículo
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