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Love Wave Immunosensor for the Detection of Carbaryl Pesticide

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Love Wave Immunosensor for the Detection of Carbaryl Pesticide

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dc.contributor.author Rocha-Gaso, María-Isabel es_ES
dc.contributor.author García Narbón, José Vicente es_ES
dc.contributor.author García, Pablo es_ES
dc.contributor.author March Iborra, Mª Del Carmen es_ES
dc.contributor.author Jiménez Jiménez, Yolanda es_ES
dc.contributor.author Francis, Laurent-Alain es_ES
dc.contributor.author Montoya Baides, Ángel es_ES
dc.contributor.author Arnau Vives, Antonio es_ES
dc.date.accessioned 2015-07-06T11:12:24Z
dc.date.available 2015-07-06T11:12:24Z
dc.date.issued 2014-09-03
dc.identifier.issn 1424-8220
dc.identifier.uri http://hdl.handle.net/10251/52737
dc.description.abstract A Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14–1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels. es_ES
dc.description.sponsorship The author also would like to acknowledge the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (ERDF) for their financing support through the grant of the INNPACTO 2012 project (DETECTA IPT-2012-0154-300000), J.V. Garcia's Fellowship, AP2007-03745, of FPU (Formacion de Profesorado Universitario) program, M. I. Rocha-Gaso's PhD CONACyT Fellowship and AWSensors Inc. for its collaboration. en_EN
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation Spanish Ministry of Economy and Competitiveness es_ES
dc.relation European Regional Development Fund (ERDF) DETECTA IPT-2012-0154-300000 es_ES
dc.relation FPU (Formacion de Profesorado Universitario) program AP2007-03745 es_ES
dc.relation PhD CONACyT Fellowship es_ES
dc.relation AWSensors Inc. es_ES
dc.relation.ispartof Sensors es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Biosensor es_ES
dc.subject Carbaryl pesticide detection es_ES
dc.subject Immunosensor es_ES
dc.subject Love Wave sensor es_ES
dc.subject Surface Acoustic Wave (SAW) es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Love Wave Immunosensor for the Detection of Carbaryl Pesticide es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/s140916434
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.description.bibliographicCitation Rocha-Gaso, M.; García Narbón, JV.; García, P.; March Iborra, MDC.; Jiménez Jiménez, Y.; Francis, L.; Montoya Baides, Á.... (2014). Love Wave Immunosensor for the Detection of Carbaryl Pesticide. Sensors. 14(9):16434-16453. doi:10.3390/s140916434 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.3390/s140916434 es_ES
dc.description.upvformatpinicio 16434 es_ES
dc.description.upvformatpfin 16453 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 269153
dc.identifier.pmid 25192313 en_EN
dc.identifier.pmcid PMC4208181 en_EN


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