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A fractal nature for polymerized laminin

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A fractal nature for polymerized laminin

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dc.contributor.author Hochman Méndez, Carlos es_ES
dc.contributor.author Cantini ., Marco es_ES
dc.contributor.author Moratal Pérez, David es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Coelho-Sampaio, Tatiana es_ES
dc.date.accessioned 2016-01-13T11:05:07Z
dc.date.available 2016-01-13T11:05:07Z
dc.date.issued 2014-10
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/59795
dc.description.abstract Polylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in animal models of spinal cord injury. Here we used confocal fluorescence microscopy (CFM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) to characterize its three-dimensional structure. Renderization of confocal optical slices of immunostained polyLM revealed the aspect of a loose flocculated meshwork, which was homogeneously stained by the antibody. On the other hand, an ordinary matrix obtained upon adsorption of laminin in neutral pH (LM) was constituted of bulky protein aggregates whose interior was not accessible to the same anti-laminin antibody. SEM and AFM analyses revealed that the seed unit of polyLM was a flat polygon formed in solution whereas the seed structure of LM was highly heterogeneous, intercalating rod-like, spherical and thin spread lamellar deposits. As polyLM was visualized at progressively increasing magnifications, we observed that the morphology of the polymer was alike independently of the magnification used for the observation. A search for the Hausdorff dimension in images of the two matrices showed that polyLM, but not LM, presented fractal dimensions of 1.55, 1.62 and 1.70 after 1, 8 and 12 hours of adsorption, respectively. Data in the present work suggest that the intrinsic fractal nature of polymerized laminin can be the structural basis for the fractal-like organization of basement membranes in the neurogenic niches of the central nervous system. es_ES
dc.description.sponsorship This work was supported by a grant from the Brazilian National Research Council (CNPq; 476772/2008-7) to TCS. MSS acknowledges support from the European Research Council through ERC - 306990. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation European Research Council through ERC - 306990 es_ES
dc.relation Brazilian National Research Council (CNPq; 476772/2008-7) es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Polylaminin (polyLM) es_ES
dc.subject Confocal fluorescence microscopy (CFM) es_ES
dc.subject Scanning electron microscopy (SEM) es_ES
dc.subject Atomic Force Microscopy (AFM) es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title A fractal nature for polymerized laminin es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0109388
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/306990/EU en_EN
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hochman Méndez, C.; Cantini ., M.; Moratal Pérez, D.; Salmerón Sánchez, M.; Coelho-Sampaio, T. (2014). A fractal nature for polymerized laminin. PLoS ONE. 9(10):109388-1-109388-11. https://doi.org/10.1371/journal.pone.0109388 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.pone.0109388 es_ES
dc.description.upvformatpinicio 109388-1 es_ES
dc.description.upvformatpfin 109388-11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 10 es_ES
dc.relation.senia 279484 es_ES
dc.identifier.pmid 25296244 en_EN
dc.identifier.pmcid PMC4190072 en_EN


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