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Influence of extraction methods on proteins and carbohydrates analysis from MBR activated sludge flocs in view of improving EPS determination

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Influence of extraction methods on proteins and carbohydrates analysis from MBR activated sludge flocs in view of improving EPS determination

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dc.contributor.author Zuriaga Agustí, Elena es_ES
dc.contributor.author Bes Piá, Mª Amparo es_ES
dc.contributor.author Mendoza Roca, José Antonio es_ES
dc.contributor.author Alonso Molina, José Luís es_ES
dc.date.accessioned 2016-01-19T08:07:15Z
dc.date.available 2016-01-19T08:07:15Z
dc.date.issued 2013-09-10
dc.identifier.issn 1383-5866
dc.identifier.uri http://hdl.handle.net/10251/60013
dc.description.abstract One of the main operational problems in membrane bioreactors (MBRs) is membrane fouling. It can be stated that extracellular polymeric substances (EPSs) analysis is of paramount importance for membrane fouling prediction and subsequently to enhance the performance of the MBR process. In this way, the standardization of EPS separation and their analytical procedures is a non-solved problem that plays an important role in order to be able of comparing data of pilot and full-scale bioreactors. Prior to unveil this fact, it is necessary to assure the accuracy of the analyzed data. In this paper, the Standard Addition Method (SAM) is applied with the aim of studying eventual interferences by other mixed liquor substances or by reagents used in the extraction procedures on the analytical methods for determining proteins and carbohydrates and as a consequence, to improve the data accuracy and reliability. For that, several extraction protocols were applied and compared in order to obtain the EPS from the activated sludge. Moreover, DNA was measured and viability tests were carried out to evaluate cell lysis extent. BCA and Lowry methods were used to quantify proteins, meanwhile Dubois and Anthrone were applied for carbohydrates. Results showed that Triton X-100 achieved the highest extraction values in terms of proteins (81.64 ± 12.9 mg BSA/g VSS) and carbohydrates (10.3 ± 1.4 mg Glucose/g VSS) concentrations linked to lower DNA values (4.9 ± 0.8 mg DNA/g VSS). In addition, epifluorescence microscope images showed the high extent of the extraction achieved. For this protocol, BCA and Dubois analytical methods were chosen since SAM found out interferences with Anthrone and Lowry, and consequently, they were recommended for data comparison in MBR plants, and for fouling prediction. es_ES
dc.description.sponsorship This research was supported by Universitat Politecnica de Valencia PAID 3260 and Generalitat Valenciana GV/2009/017 and Spanish Ministry of Economy and Competitiveness (Grant No. CTQ2011-29231-C05-02). en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Universitat Politecnica de Valencia PAID 3260 es_ES
dc.relation Generalitat Valenciana GV/2009/017 es_ES
dc.relation Spanish Ministry of Economy and Competitiveness CTQ2011-29231-C05-02 es_ES
dc.relation.ispartof Separation and Purification Technology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Standard Addition Method es_ES
dc.subject EPS es_ES
dc.subject SMP es_ES
dc.subject Extraction methods es_ES
dc.subject MBR es_ES
dc.subject.classification INGENIERIA QUIMICA es_ES
dc.title Influence of extraction methods on proteins and carbohydrates analysis from MBR activated sludge flocs in view of improving EPS determination es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.seppur.2013.03.048
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Seguridad Industrial, Radiofísica y Medioambiental - Institut de Seguretat Industrial, Radiofísica i Mediambiental es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.description.bibliographicCitation Zuriaga Agusti, E.; Bes Piá, MA.; Mendoza Roca, JA.; Alonso Molina, JL. (2013). Influence of extraction methods on proteins and carbohydrates analysis from MBR activated sludge flocs in view of improving EPS determination. Separation and Purification Technology. 112:1-10. doi:10.1016/j.seppur.2013.03.048 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.seppur.2013.03.048 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 10 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 112 es_ES
dc.relation.senia 255066 es_ES


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