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Enzyme-Responsive Intracellular-Controlled Release Using Silica Mesoporous Nanoparticles Capped with e-Poly-l-lysine

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Enzyme-Responsive Intracellular-Controlled Release Using Silica Mesoporous Nanoparticles Capped with e-Poly-l-lysine

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dc.contributor.author Mondragón Martínez, Laura es_ES
dc.contributor.author Mas Font, Nuria es_ES
dc.contributor.author Ferragud, Vicente es_ES
dc.contributor.author de la Torre, Cristina es_ES
dc.contributor.author Agostini, Alessandro es_ES
dc.contributor.author Martínez Mañez, Ramón es_ES
dc.contributor.author Sancenón Galarza, Félix es_ES
dc.contributor.author Amoros del Toro, Pedro Jose es_ES
dc.contributor.author Pérez Payá, Enrique es_ES
dc.contributor.author Orzáez Calatayud, Mar es_ES
dc.date.accessioned 2016-01-21T09:31:16Z
dc.date.issued 2014-04-25
dc.identifier.issn 0947-6539
dc.identifier.uri http://hdl.handle.net/10251/60096
dc.description.abstract The synthesis and characterization of two new capped silica mesoporous nanoparticles for controlled delivery purposes are described. Capped hybrid systems consist of MCM-41 nanoparticles functionalized on the outer surface with polymer epsilon-poly-L-lysine by two different anchoring strategies. In both cases, nanoparticles were loaded with model dye molecule [Ru(bipy)(3)](2+). An anchoring strategy involved the random formation of urea bonds by the treatment of propyl isocyanate-functionalized MCM-41 nanoparticles with the lysine amino groups located on the epsilon-poly-L-lysine backbone (solid Ru-rLys-S1). The second strategy involved a specific attachment through the carboxyl terminus of the polypeptide with azidopropyl-functionalized MCM-41 nanoparticles (solid Ru-tLys-S1). Once synthesized, both nanoparticles showed a nearly zero cargo release in water due to the coverage of the nanoparticle surface by polymer epsilon-poly-L-lysine. In contrast, a remarkable payload delivery was observed in the presence of proteases due to the hydrolysis of the polymer's amide bonds. Once chemically characterized, studies of the viability and the lysosomal enzyme-controlled release of the dye in intracellular media were carried out. Finally, the possibility of using these materials as drug-delivery systems was tested by preparing the corresponding epsilon-poly-L-lysine capped mesoporous silica nanoparticles loaded with cytotoxic drug camptothecin (CPT), CPT-rLys-S1 and CPT-tLys-S1. Cellular uptake and cell-death induction were studied. The efficiency of both nanoparticles as new potential platforms for cancer treatment was demonstrated. es_ES
dc.description.sponsorship We thank the Spanish Government (Project MAT2012-38429-C04, CTQ2007-64735-AR07 and SAF2010-15512) and the Generalitat Valenciana (Project PROMETEO/2009/016) for support. L.M. and A.A. thank the Generalitat Valenciana for their postdoctoral contract VALI+D and Santiago Grisolia PhD fellowship, respectively. N.M. thanks The Spanish Ministry of Science and Innovation for her FPI fellowship. C.T. thanks the Universitat Politecnica de Valencia for her PhD fellowship. M.O. thanks the CIPF for her postdoctoral fellowship. We thank the confocal microscopy service, Alberto Hernandez and EvaMaria LaFuente from CIPF for their technical support. en_EN
dc.language Inglés es_ES
dc.publisher Wiley-VCH Verlag es_ES
dc.relation Spanish Government MAT2012-38429-C04 CTQ2007-64735-AR07 SAF2010-15512 es_ES
dc.relation Generalitat Valenciana PROMETEO/2009/016 es_ES
dc.relation Spanish Ministry of Science and Innovation es_ES
dc.relation Universitat Politecnica de Valencia es_ES
dc.relation CIPF es_ES
dc.relation.ispartof Chemistry - A European Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject anchoring strategy es_ES
dc.subject intracellular release es_ES
dc.subject mesoporous materials es_ES
dc.subject nanoparticles es_ES
dc.subject poly-L-lysine es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Enzyme-Responsive Intracellular-Controlled Release Using Silica Mesoporous Nanoparticles Capped with e-Poly-l-lysine es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1002/chem.201400148
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Mondragón Martínez, L.; Mas Font, N.; Ferragud, V.; De La Torre, C.; Agostini, A.; Martínez Mañez, R.; Sancenón Galarza, F.... (2014). Enzyme-Responsive Intracellular-Controlled Release Using Silica Mesoporous Nanoparticles Capped with e-Poly-l-lysine. Chemistry - A European Journal. 20(18):5271-5281. doi:10.1002/chem.201400148 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1002/chem.201400148 es_ES
dc.description.upvformatpinicio 5271 es_ES
dc.description.upvformatpfin 5281 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 20 es_ES
dc.description.issue 18 es_ES
dc.relation.senia 276587 es_ES
dc.identifier.eissn 1521-3765


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