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Comparison of protocols for DNA extraction from Cannabis sativa seeds

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Comparison of protocols for DNA extraction from Cannabis sativa seeds

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dc.contributor.author Soler Aleixandre, Salvador es_ES
dc.contributor.author Sifres Cuerda, Alicia Gemma es_ES
dc.contributor.author Llosa, Ernesto R. es_ES
dc.contributor.author Llamas, Matías es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.date.accessioned 2016-01-27T10:03:00Z
dc.date.available 2016-01-27T10:03:00Z
dc.date.issued 2013
dc.identifier.issn 1843-5254
dc.identifier.uri http://hdl.handle.net/10251/60239
dc.description.abstract Cannabis sativa is an important crop cultivated both for industrial (var. sativa) and medicinal and recreational (var. indica) purposes. Cultivation of var. indica plants is generally forbidden and this difficults genetic studies. An alternative is the extraction of DNA from seed embryos. In order to develop an efficient protocol for DNA extraction of C. sativa seeds we have tested six DNA extraction methods in seeds and leaves of a C. sativa var. sativa accession. We found that the best protocol is the CTAB-modified with phenol:chlorophorm:isoamyl washing, which allowed a large quantity of high quality DNA per seed. This method was tested in seeds of three C. sativa var. indica accessions and seven SSR markers tested could be amplified successfully. In summary, we have developed a highly efficient method for DNA extraction of individual seeds of C. sativa. This method will be useful for genetic studies in this species. es_ES
dc.language Inglés es_ES
dc.publisher University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca es_ES
dc.relation.ispartof Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Horticulture es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject CTAB es_ES
dc.subject Embryos es_ES
dc.subject Extraction kits es_ES
dc.subject Molecular markers es_ES
dc.subject SSRs es_ES
dc.subject.classification GENETICA es_ES
dc.title Comparison of protocols for DNA extraction from Cannabis sativa seeds es_ES
dc.type Artículo es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Soler Aleixandre, S.; Sifres Cuerda, AG.; Llosa, ER.; Llamas, M.; Vilanova Navarro, S.; Prohens Tomás, J. (2013). Comparison of protocols for DNA extraction from Cannabis sativa seeds. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Horticulture. 70(1):265-266. http://hdl.handle.net/10251/60239 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://journals.usamvcluj.ro/index.php/horticulture/article/view/9255 es_ES
dc.description.upvformatpinicio 265 es_ES
dc.description.upvformatpfin 266 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 70 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 254915 es_ES


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