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Destabilization and removal of immobilized enzymes adsorbed onto polyethersulfone ultrafiltration membranes by salt solutions

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Destabilization and removal of immobilized enzymes adsorbed onto polyethersulfone ultrafiltration membranes by salt solutions

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dc.contributor.author Corbatón Báguena, María José es_ES
dc.contributor.author Gugliuzza, A. es_ES
dc.contributor.author Cassano, A. es_ES
dc.contributor.author Mazzei, R. es_ES
dc.contributor.author Giorno, L. es_ES
dc.date.accessioned 2016-01-29T08:38:00Z
dc.date.available 2016-01-29T08:38:00Z
dc.date.issued 2015-07-15
dc.identifier.issn 0376-7388
dc.identifier.uri http://hdl.handle.net/10251/60347
dc.description.abstract In this work the effectiveness of two saline solutions (NaCl and Na2SO4) to clean a permanently hydrophilic polyethersulfone (PESH) ultrafiltration (UF) membrane with a molecular weight cut-off (MWCO) of 30 kDa previously fouled with enzymatic solutions was investigated. The influence of protein concentration in the enzymatic solution during the fouling step and the effect of salt type during the cleaning procedure were studied. The protein aggregation was analyzed in solution and onto the membrane surface by using several techniques including Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and Infrared Spectroscopy with Attenuated Total Reflectance (ATR-FTIR). In addition, mechanisms that dominate membrane fouling were studied by fitting some mathematical models (Hermia's models adapted to crossflow filtration, a combined model based on the complete blocking and cake formation equations and a resistance-in-series model) to the experimental data. Fouling results showed that the complete blocking/adsorption on membrane surface was the predominant fouling mechanism. Regarding the cleaning results, higher cleaning efficiency and low residual protein concentration was obtained with NaCl solutions for all the feed solutions tested due to the favorable interaction between Cl and proteins. es_ES
dc.description.sponsorship Maria-Jose Corbaton-Baguena wishes to gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the grant EEBB-I-14-09011 (project CTM2010-20186). The authors acknowledge the European Union, Fondo Europeo di Sviluppo Regionale, The Ministero dell'Istruzione, dell'Universita e della Ricerca - MIUR, The Ministero dello Svilupppo Economic - MSE - for the financial support to the project "Sistemi tecnologici avanzati e processi integrati della filiera olivicola per la valorizzazione dei prodotti e dei sottoprodotti, lo sviluppo di nuovi settori e la creazione di sistemi produttivi Eco-compatibili" (PON Olio Piu, PON01_01545), within the framework PON Ricerca e Competitivita 2007-2013. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Membrane Science es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Membrane cleaning es_ES
dc.subject Protein fouling es_ES
dc.subject Salt solutions es_ES
dc.subject Ultrafiltration es_ES
dc.subject Mathematical models es_ES
dc.subject.classification INGENIERIA QUIMICA es_ES
dc.title Destabilization and removal of immobilized enzymes adsorbed onto polyethersulfone ultrafiltration membranes by salt solutions es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.memsci.2015.03.061
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//CTM2010-20186/ES/TECNICAS NO CONVENCIONALES PARA LA LIMPIEZA DE MEMBRANAS DE ULTRAFILTRACION APLICADAS EN LA INDUSTRIA ALIMENTARIA/ / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MiSE//PON01_01545/IT/Sistemi tecnologici avanzati e processi integrati nella filiera olivicola per la valorizzazione dei prodotti e dei sottoprodotti, lo sviluppo di nuovi settori e la creazione di sistemi produttivi eco-compatibili/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//EEBB-I-14-09011/ES/EEBB-I-14-09011/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear es_ES
dc.description.bibliographicCitation Corbatón Báguena, MJ.; Gugliuzza, A.; Cassano, A.; Mazzei, R.; Giorno, L. (2015). Destabilization and removal of immobilized enzymes adsorbed onto polyethersulfone ultrafiltration membranes by salt solutions. Journal of Membrane Science. 486:207-214. https://doi.org/10.1016/j.memsci.2015.03.061 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.memsci.2015.03.061 es_ES
dc.description.upvformatpinicio 207 es_ES
dc.description.upvformatpfin 214 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 486 es_ES
dc.relation.senia 286393 es_ES
dc.contributor.funder Ministero dello Sviluppo Economico es_ES
dc.contributor.funder Ministero dell'Istruzione dell'Università e della Ricerca, Italia es_ES
dc.contributor.funder European Regional Development Fund es_ES


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