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Ultrastructural and histochemical analysis reveals ethylene-induced responses underlying reduced peel collapse in detached citrus fruit

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Ultrastructural and histochemical analysis reveals ethylene-induced responses underlying reduced peel collapse in detached citrus fruit

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dc.contributor.author Cajuste, J.F. es_ES
dc.contributor.author García Breijo, Francisco José es_ES
dc.contributor.author Reig Armiñana, José es_ES
dc.contributor.author Lafuente, M.T. es_ES
dc.date.accessioned 2016-03-09T12:48:24Z
dc.date.available 2016-03-09T12:48:24Z
dc.date.issued 2011-10
dc.identifier.issn 1059-910X
dc.identifier.uri http://hdl.handle.net/10251/61608
dc.description This is the accepted version of the following article: Cajuste, J.; García Breijo, FJ.; Reig Armiñana, J.; Lafuente, M. (2011). Ultrastructural and histochemical analysis reveals ethylene-induced responses underlying reduced peel collapse in detached citrus fruit. Microscopy Research and Technique. 74(1):970-979, which has been published in final form at http://dx.doi.org/10.1002/jemt.20983. es_ES
dc.description.abstract Fruits from many citrus cultivars develop depressed areas in the flavedo (outer part of the peel) and albedo (inner part) following detachment. Although ultrastructural analysis may provide important information about multiple plant responses to stresses and external stimuli at the cell and tissue levels, and despite the proved efficacy of ethylene in reducing peel damage in citrus fruit, cytological responses of this horticultural crop to protective ethylene concentrations have not yet been reported. We show that applying high ethylene levels (2 mu L L(-1) for 14 days) causes sublethal stress as it favored the alteration of cuticle, vacuole, middle lamella and primary wall, especially in the albedo cells, but reduced peel collapse in detached mature "Navelate" oranges (C. sinensis, L. Osbeck) held under nonstressful environmental conditions (22 degrees C and 90-95% RH). Ethylene did not induce relevant changes in lignification but favored the deposition of pectic exudates and the release of sugars from degradation of cell polysaccharides including starch, cellulose, and pectins. In contrast, inhibiting ethylene perception by applying 1-methylcyclopropene (1-MCP) reduced these ethylene-related responses and favored degradation of cell membranes and peel damage. The overall results reflect that mature oranges tolerate high ethylene levels that might favor the activation of defense responses involving oxidative-stress related mechanisms and recycling of nutrients and carbon supply to enable cells to sustain respiration and cope with carbon deprivation stress caused by detachment. Microsc. Res. Tech. 74:970-979, 2011. (C) 2011 Wiley-Liss, Inc. es_ES
dc.description.sponsorship Contract grant sponsor: Comision Interministerial de Ciencia y Tecnologia (CICYT), Spain; Contract grant number: AGL2002-1727; Contract grant number: AGL2009-11969; Contract grant sponsor: Conselleria D'Educacio Generalitat Valenciana, Spain; Contract grant number: PROMETEO/2010/010; Contract grant sponsor: SUPERA Programme, Mexico en_EN
dc.language Inglés es_ES
dc.publisher Wiley-Blackwell es_ES
dc.relation.ispartof Microscopy Research and Technique es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cell ultrastructure es_ES
dc.subject Cross-protection es_ES
dc.subject Pectin es_ES
dc.subject Peel damage es_ES
dc.subject Polysaccharides es_ES
dc.subject Starch es_ES
dc.subject Electron Microscopy Service of the UPV es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification BIOLOGIA VEGETAL es_ES
dc.title Ultrastructural and histochemical analysis reveals ethylene-induced responses underlying reduced peel collapse in detached citrus fruit es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/jemt.20983
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2009-11969/ES/Bases Moleculares Y Metabolismo De Fosfolipidos En Alteraciones Fisiologicas Postcosecha De Frutos Citricos/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F010/ES/Nuevas aproximaciones fisiológicas y biotecnológicas en postcosecha de frutos/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICYT//AGL2002-1727/ES/RESISTENCIA A ALTERACIONES FISIOLOGICAS Y PATOLOGICAS DURANTE LAS POSTCOSECHA DE LOS FRUTOS CITRICOS: BASES MOLECULARES Y METABOLISMO DE FENILPROPANOIDES/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.description.bibliographicCitation Cajuste, J.; García Breijo, FJ.; Reig Armiñana, J.; Lafuente, M. (2011). Ultrastructural and histochemical analysis reveals ethylene-induced responses underlying reduced peel collapse in detached citrus fruit. Microscopy Research and Technique. 74(10):970-979. https://doi.org/10.1002/jemt.20983 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1002/jemt.20983 es_ES
dc.description.upvformatpinicio 970 es_ES
dc.description.upvformatpfin 979 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 74 es_ES
dc.description.issue 10 es_ES
dc.relation.senia 39042 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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