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The moment protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the Sw-5 gene-based resistance

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The moment protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the Sw-5 gene-based resistance

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dc.contributor.author Peiró Morell, Ana es_ES
dc.contributor.author Cañizares, María del Carmen es_ES
dc.contributor.author Rubio, Luis es_ES
dc.contributor.author López Del Rincón, Carmelo es_ES
dc.contributor.author Moriones, Enrique es_ES
dc.contributor.author Aramburu, José es_ES
dc.contributor.author Sanchez Navarro, Jesus Angel es_ES
dc.date.accessioned 2016-03-09T15:32:15Z
dc.date.available 2016-03-09T15:32:15Z
dc.date.issued 2014-10
dc.identifier.issn 1464-6722
dc.identifier.uri http://hdl.handle.net/10251/61622
dc.description This is the accepted version of the following article: Peiró Morell, A.; Cañizares, MC.; Rubio, L.; López Del Rincón, C.; Moriones, E.; Aramburu, J.; Sanchez Navarro, JA. (2014). The moment protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the Sw-5 gene-based resistance. Molecular Plant Pathology. 15:802-813. doi:10.1111/mpp.12142., which has been published in final form at http://dx.doi.org/10.1111/mpp.12142 . es_ES
dc.description.abstract The avirulence determinant triggering the resistance conferred by the tomato gene Sw-5 against Tomato spotted wilt virus (TSWV) is still unresolved. Sequence comparison showed two substitutions (C118Y and T120N) in the movement protein NSm present only in TSWV resistance-breaking (RB) isolates. In this work, transient expression of NSm of three TSWV isolates [RB1 (T120N), RB2 (C118Y) and non-resistance-breaking (NRB)] in Nicotiana benthamiana expressing Sw-5 showed a hypersensitive response (HR) only with NRB. Exchange of the movement protein of Alfalfa mosaic virus (AMV) with NSm supported cell-to-cell and systemic transport of the chimeric AMV RNAs into N.tabacum with or without Sw-5, except for the constructs with NBR when Sw-5 was expressed, although RB2 showed reduced cell-to-cell transport. Mutational analysis revealed that N120 was sufficient to avoid the HR, but the substitution V130I was required for systemic transport. Finally, co-inoculation of RB and NRB AMV chimeric constructs showed different prevalence of RB or NBR depending on the presence or absence of Sw-5. These results indicate that NSm is the avirulence determinant for Sw-5 resistance, and mutations C118Y and T120N are responsible for resistance breakdown and have a fitness penalty in the context of the heterologous AMV system. es_ES
dc.description.sponsorship A.P. was a recipient of a JAE-Pre contract from the Consejo Superior de Investigaciones Cientificas (CSIC), and M. C. C was a recipient of an I3P contract from CSIC (co-financed by Fondo Social Europeo, FSE). We thank L. Corachan for excellent technical assistance and Dr Marcel Prins for providing the Nt/Sw5-b and Nb/Sw5-b seeds. This work was supported by grant BIO2011-25018 from the Spanish granting agency DGICYT, grant PAID05-11/2888 from the Universidad Politecnica de Valencia and by grant RTA2008-00010-C03 from the Instituto Nacional de Investigaciones Agrarias (INIA). All authors have no conflicts of interest to declare. en_EN
dc.language Inglés es_ES
dc.publisher Wiley-Blackwell es_ES
dc.relation.ispartof Molecular Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Avr es_ES
dc.subject Competition assays es_ES
dc.subject Movement protein NSm es_ES
dc.subject Tospovirus es_ES
dc.subject Transient expression es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title The moment protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the Sw-5 gene-based resistance es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/mpp.12142
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-25018/ES/TRAFICO INTRACELULAR, INTERCELULAR Y VASCULAR DE RNAS Y PROTEINAS VIRALES Y SUBVIRALES EN PLANTAS¿/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2008-00010-C03-01/ES/Diversidad genética y factores evolutivos y epidemiológicos implicados en los aislados españoles de TSWV que superan las resistencias genéticas Sw-5 de tomate y Tsw de pimiento/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-05-11-2888/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2008-00010-C03-02/ES/Caracterización de aislados del virus del bronceado (TSWV) que sobrepasan la resistencia del gen Tsw en pimiento. Puesta a punto de la utilización de RNA de interferencia como método de control/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2008-00010-C03-03/ES/Estudio de los determinantes genéticos de TSWV implicados en la superación de las resistencias de tomate y pimiento. Desarrollo de nuevas variedades resistentes/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Peiró Morell, A.; Cañizares, MDC.; Rubio, L.; López Del Rincón, C.; Moriones, E.; Aramburu, J.; Sanchez Navarro, JA. (2014). The moment protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the Sw-5 gene-based resistance. Molecular Plant Pathology. 15(8):802-813. https://doi.org/10.1111/mpp.12142 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1111/mpp.12142 es_ES
dc.description.upvformatpinicio 802 es_ES
dc.description.upvformatpfin 813 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 15 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 277784 es_ES
dc.identifier.eissn 1364-3703
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
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