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Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages

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Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages

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dc.contributor.author Jiménez Gasco, María del Mar es_ES
dc.contributor.author Malcolm, Glenna M. es_ES
dc.contributor.author Berbegal Martínez, Mónica es_ES
dc.contributor.author Armengol Fortí, Josep es_ES
dc.contributor.author JIMENEZ DIAZ, RAFAEL es_ES
dc.date.accessioned 2016-03-29T14:23:32Z
dc.date.available 2016-03-29T14:23:32Z
dc.date.issued 2014-06
dc.identifier.issn 0031-949X
dc.identifier.uri http://hdl.handle.net/10251/62065
dc.description.abstract Verticillium wilts caused by the soilborne fungus Verticillium dahliae are among the most challenging diseases to control. Populations of this pathogen have been traditionally studied by means of vegetative compatibility groups (VCGs) under the assumption that VCGs comprise genetically related isolates that correlate with clonal lineages. We aimed to resolve the phylogenetic relationships among VCGs and their subgroups based on sequences of the intergenic spacer region (IGS) of the ribosomal DNA and six anonymous polymorphic sequences containing single-nucleotide polymorphisms (VdSNPs). A collection of 68 V dahliae isolates representing the main VCGs and subgroups (VCGs 1A, 1B, 2A, 2B, 3, 4A, 4B, and 6) from different geographic origins and hosts was analyzed using the seven DNA regions. Maximum parsimony (MP) phylogenies inferred from IGS and VdSNP sequences showed five and six distinct clades, respectively. Phylogenetic analyses of individual and combined data sets indicated that certain VCG subgroups (e.g., VCGs 1A and 1B) are closely related and share a common ancestor; however, other subgroups (e.g., VCG 4B) are more closely related to members of a different VCG (e.g., VCG 2A) than to subgroups of the same VCG (VCG 4B). Furthermore, MP analyses indicated that VCG 2B is polyphyletic, with isolates placed in at least three distinct phylogenetic lineages based on IGS sequences and two lineages based on VdSNP sequences. Results from our study suggest the existence of main VCG lineages that contain VCGs 1A and 1B; VCGs 2A and 4B; and VCG 4A, for which both phylogenies agree; and the existence of other VCGs or VCG subgroups that seem to be genetically heterogeneous or show discrepancies in their phylogenetic placement: VCG 2B, VCG 3, and VCG 6. These results raise important caveats regarding the interpretation of VCG analyses: genetic homogeneity and close evolutionary relationship between members of a VCG should not be assumed. es_ES
dc.description.sponsorship This research was partially funded by the Sarah Chinn Kalser Faculty Research Assistance Endowment, College of Agricultural Sciences, The Pennsylvania State University. We thank all suppliers of V. dahliae isolates; J. Yanez, S. Colihan, C. Barrett, C. Black, and C. Olivares-Garcia for excellent technical support; and D. Geiser for helpful discussions during the preparation of this manuscript. en_EN
dc.language Inglés es_ES
dc.publisher American Phytopathological Society es_ES
dc.relation Sarah Chinn Kalser Faculty Research Assistance Endowment, College of Agricultural Sciences, The Pennsylvania State University es_ES
dc.relation.ispartof Phytopathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject Genetic diversity es_ES
dc.subject Heterokaryon incompatibility es_ES
dc.subject Tester strains es_ES
dc.subject DNA sequence es_ES
dc.subject Wilt es_ES
dc.subject Potato es_ES
dc.subject Populations es_ES
dc.subject Olive es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.subject.classification ECOSISTEMAS AGROFORESTALES (UPV) es_ES
dc.title Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1094/PHYTO-07-13-0180-R
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation Jiménez Gasco, MDM.; Malcolm, GM.; Berbegal Martinez, M.; Armengol Fortí, J.; Jimenez Diaz, R. (2014). Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages. Phytopathology. 104(6):650-659. doi:10.1094/PHYTO-07-13-0180-R es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1094/PHYTO-07-13-0180-R es_ES
dc.description.upvformatpinicio 650 es_ES
dc.description.upvformatpfin 659 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 104 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 279657 es_ES


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