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Robust fabrication of electrospun-like polymer mats to direct cell behaviour

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Robust fabrication of electrospun-like polymer mats to direct cell behaviour

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dc.contributor.author Ballester Beltrán, José es_ES
dc.contributor.author Lebourg., Myriam Madeleine es_ES
dc.contributor.author Capella Monsonís, Héctor es_ES
dc.contributor.author Díaz Lantada, Andrés es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2016-04-06T11:33:38Z
dc.date.available 2016-04-06T11:33:38Z
dc.date.issued 2014-09
dc.identifier.issn 1758-5082
dc.identifier.uri http://hdl.handle.net/10251/62291
dc.description.abstract Currently, cell culture systems that include nanoscale topography are widely used in order to provide cells additional cues closer to the in vivo environment, seeking to mimic the natural extracellular matrix. Electrospinning is one of the most common techniques to produce nanofiber mats. However, since many sensitive parameters play an important role in the process, a lack of reproducibility is a major drawback. Here we present a simple and robust methodology to prepare reproducible electrospun-like samples. It consists of a polydimethylsiloxane mold reproducing the fiber pattern to solvent-cast a polymer solution and obtain the final sample. To validate this methodology, poly( L-lactic) acid ( PLLA) samples were obtained and, after characterisation, bioactivity and ability to direct cell response were assessed. C2C12 myoblasts developed focal adhesions on the electrospun-like fibers and, when cultured under myogenic differentiation conditions, similar differentiation levels to electrospun PLLA fibers were obtained. es_ES
dc.description.sponsorship The support of ERC through HealInSynergy (306990) and FPU program AP2009-3626 is acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher IOP Publishing: Hybrid Open Access es_ES
dc.relation.ispartof Biofabrication es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Biophysical cues es_ES
dc.subject Cell adhesion es_ES
dc.subject Microfabrication es_ES
dc.subject Cell alignment es_ES
dc.subject Electrospinning es_ES
dc.subject Myogenic differentiation es_ES
dc.subject Poly(lactic acid) es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Robust fabrication of electrospun-like polymer mats to direct cell behaviour es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1088/1758-5082/6/3/035009
dc.relation.projectID info:eu-repo/grantAgreement/ME//AP2009-3626/ES/AP2009-3626/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/306990/EU/Material-driven Fibronectin Fibrillogenesis to Engineer Synergistic Growth Factor Microenvironments/ en_EN
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Ballester Beltrán, J.; Lebourg., MM.; Capella Monsonís, H.; Díaz Lantada, A.; Salmerón Sánchez, M. (2014). Robust fabrication of electrospun-like polymer mats to direct cell behaviour. Biofabrication. 6(3). https://doi.org/10.1088/1758-5082/6/3/035009 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1088/1758-5082/6/3/035009 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 278624 es_ES
dc.identifier.eissn 1758-5090
dc.contributor.funder Ministerio de Educación es_ES
dc.contributor.funder European Commission


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