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Determination of phytoplankton composition using absorption spectra

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Determination of phytoplankton composition using absorption spectra

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dc.contributor.author Martínez Guijarro, Mª Remedios es_ES
dc.contributor.author Romero Gil, Inmaculada es_ES
dc.contributor.author Pachés Giner, María Aguas Vivas es_ES
dc.contributor.author González del Rio Rams, Julio es_ES
dc.contributor.author Martí Insa, Carmen Mª es_ES
dc.contributor.author GIL SEGUÍ, GERMA es_ES
dc.contributor.author Ferrer, Alberto es_ES
dc.contributor.author FERRER, J. es_ES
dc.date.accessioned 2016-04-13T16:29:08Z
dc.date.available 2016-04-13T16:29:08Z
dc.date.issued 2009
dc.identifier.issn 0039-9140
dc.identifier.uri http://hdl.handle.net/10251/62526
dc.description.abstract Characterisation of phytoplankton communities in aquatic ecosystems is a costly task in terms of time, material and human resources. The general objective of this paper is not to replace microscopic counts but to complement them, by fine-tuning a technique using absorption spectra measurements that reduces the above-mentioned costs. Therefore, the objective proposed in this paper is to assess the possibility of achieving a qualitative determination of phytoplankton communities by classes, and also a quantitative estimation of the number of phytoplankton cells within each of these classes, using spectrophotometric determination. Samples were taken in three areas of the Spanish Mediterranean coast. These areas correspond to estuary systems that are influenced by both continental waters and Mediterranean Sea waters. 139 Samples were taken in 7–8 stations per area, at different depths in each station. In each sample, the absorption spectrum and the phytoplankton classes (Bacyllariophyceae (diatoms), Cryptophyceae, Clorophyceae, Chrysophyceae, Prasynophyceae, Prymnesophyceae, Euglenophyceae, Cyanophyceae, Dynophyceae and the Synechococcus sp.) were determined. Data were analysed by means of the Partial Least Squares (PLS) multivariate statistical technique. The absorbances obtained between 400 and 750 nm were used as the independent variable and the cell/l of each phytoplankton class was used as the dependent variable, thereby obtaining models which relate the absorbance of the sample extract to the phytoplankton present in it. Good results were obtained for diatoms (Bacillarophyceae), Chlorophyceae and Cryptophyceae. © 2009 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship This research work has been supported by the Regional Ministry of the Environment, Water, Urban Planning and Housing. en_EN
dc.language Español es_ES
dc.publisher Elsevier es_ES
dc.relation Regional Ministry of the Environment, Water, Urban Planning and Housing. es_ES
dc.relation.ispartof Talanta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Absorption spectra es_ES
dc.subject Phytoplankton classes es_ES
dc.subject Partial Least Squares es_ES
dc.subject Phytoplankton pigments es_ES
dc.subject.classification ESTADISTICA E INVESTIGACION OPERATIVA es_ES
dc.subject.classification TECNOLOGIA DEL MEDIO AMBIENTE es_ES
dc.title Determination of phytoplankton composition using absorption spectra es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.talanta.2008.12.073
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Hidráulica y Medio Ambiente - Departament d'Enginyeria Hidràulica i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Estadística e Investigación Operativa Aplicadas y Calidad - Departament d'Estadística i Investigació Operativa Aplicades i Qualitat es_ES
dc.description.bibliographicCitation Martínez Guijarro, MR.; Romero Gil, I.; Paches Giner, MAV.; González Del Rio Rams, J.; Martí Insa, CM.; Gil Seguí, G.; Ferrer, A.... (2009). Determination of phytoplankton composition using absorption spectra. Talanta. 78:814-819. doi:10.1016/j.talanta.2008.12.073 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.talanta.2008.12.073 es_ES
dc.description.upvformatpinicio 814 es_ES
dc.description.upvformatpfin 819 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 78 es_ES
dc.relation.senia 37694 es_ES


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