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Quantification of viable Giardia cysts and Cryptosporidium oocysts in wastewater using propidium monoazide quantitative real-time PCR

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Quantification of viable Giardia cysts and Cryptosporidium oocysts in wastewater using propidium monoazide quantitative real-time PCR

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Alonso Molina, JL.; Amoros-Muñoz, I.; Guy, RA. (2014). Quantification of viable Giardia cysts and Cryptosporidium oocysts in wastewater using propidium monoazide quantitative real-time PCR. Parasitology Research. 113(7):2671-2678. doi:10.1007/s00436-014-3922-9

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Title: Quantification of viable Giardia cysts and Cryptosporidium oocysts in wastewater using propidium monoazide quantitative real-time PCR
Author: Alonso Molina, José Luís Amoros-Muñoz, Inmaculada Guy, Rebecca A.
UPV Unit: Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient
Issued date:
Abstract:
Real-time PCR (qPCR) is a rapid tool to quantify pathogens in the aquatic environment; however, it quantifies all pathogens, including both viable and nonviable. Propidium monoazide (PMA) is a membrane-impairment dye ...[+]
Subjects: Cryptosporidium , Giardia , PMA , QPCR , Wastewater , Viable
Copyrigths: Reserva de todos los derechos
Source:
Parasitology Research. (issn: 0932-0113 )
DOI: 10.1007/s00436-014-3922-9
Publisher:
Springer Verlag (Germany)
Publisher version: http://dx.doi.org/10.1007/s00436-014-3922-9
Project ID:
Spanish Ministerio de Ciencia e Innovacion AGL2008-05275-C03-03/ALI
Public Health Agency of Canada
Thanks:
This work was supported by the Spanish Ministerio de Ciencia e Innovacion, grant AGL2008-05275-C03-03/ALI. It was also financed, in part, by the Public Health Agency of Canada.
Type: Artículo

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