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Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

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Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

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dc.contributor.author MNATSAKANYAN, HAYK es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Grigoriou, Eleni es_ES
dc.contributor.author Maturana Candelas, Aarón es_ES
dc.contributor.author Rodrigo Navarro, Aleixandre es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Sabater i Serra, Roser es_ES
dc.date.accessioned 2016-04-28T09:59:42Z
dc.date.issued 2015-08-19
dc.identifier.issn 1944-8244
dc.identifier.uri http://hdl.handle.net/10251/63095
dc.description.abstract Fibronectin fibrillogenesis is the physiological process by which cells elaborate a fibrous FN matrix. Poly(ethyl acrylate), PEA, has been described to induce a similar process upon simple adsorption of fibronectin (FN) from a protein solution-in the absence of cells-leading to the so-called material-driven fibronectin fibrillogenesis. Poly(methyl acrylate), PMA, is a polymer with very similar chemistry to PEA, on which FN is adsorbed, keeping the globular conformation of the protein in solution. We have used radical polymerization to synthesize copolymers with controlled EA/MA ratio, seeking to modulate the degree of FN fibrillogenesis. The physicochemical properties of the system were studied using dynamic mechanical analysis, differential scanning calorimetry, and water contact angle. Both the degree of FN fibrillogenesis and the availability of the integrin binding region of FN directly depend on the percentage of EA in the copolymer, whereas the same total amount of FN was adsorbed regardless the EA/MA ratio. Cell morphology adhesion and differentiation of murine C2C12 were shown to depend on the degree of FN fibrillogenesis previously attained on the material surface. Myogenic differentiation was enhanced on the copolymers with higher EA content, i.e. more interconnected FN fibrils. es_ES
dc.description.sponsorship M.S.S. acknowledges the ERC through HealInSynergy 306990. The support of the project MAT2012-38359-C03-01 (including the FEDER financial support) and well as CIBER-BBN [CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN, promoted by IS CIII), Spain] is acknowledged. We also acknowledge the Electron Microscopy Service of the UPV for AFM support. en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN) es_ES
dc.relation FEDER/MAT/2012-38359-C03-01 es_ES
dc.relation.ispartof ACS Applied Materials and Interfaces es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Fibronectin es_ES
dc.subject Fibrillogenesis es_ES
dc.subject Biointerface es_ES
dc.subject Bioactive substrates es_ES
dc.subject Cell adhesion es_ES
dc.subject Cell differentiation es_ES
dc.subject Electron Microscopy Service of the UPV
dc.subject.classification INGENIERIA ELECTRICA es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/acsami.5b05466
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Eléctrica - Departament d'Enginyeria Elèctrica es_ES
dc.description.bibliographicCitation Mnatsakanyan, H.; Rico Tortosa, PM.; Grigoriou, E.; Maturana Candelas, A.; Rodrigo Navarro, A.; Salmerón Sánchez, M.; Sabater I Serra, R. (2015). Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry. ACS Applied Materials and Interfaces. 7(32):18125-18135. doi:10.1021/acsami.5b05466 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1021/acsami.5b05466 es_ES
dc.description.upvformatpinicio 18125 es_ES
dc.description.upvformatpfin 18135 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 32 es_ES
dc.relation.senia 296383 es_ES


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