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Gold-nanoparticle based immunoassay on a Digital Versatile Disk for the determination of priority pollutants residues in water

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Gold-nanoparticle based immunoassay on a Digital Versatile Disk for the determination of priority pollutants residues in water

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dc.contributor.advisor Maquieira Catala, Ángel es_ES
dc.contributor.advisor Morais Ezquerro, Sergi Beñat es_ES
dc.contributor.author Dobosz, Paulina es_ES
dc.date.accessioned 2016-04-28T18:39:41Z
dc.date.available 2016-04-28T18:39:41Z
dc.date.created 2013-02-13
dc.date.issued 2016-04-28
dc.identifier.uri http://hdl.handle.net/10251/63122
dc.description.abstract The aim of this work is the development of an immunoassay adapted for compact disc technology to determine the priority pollutants residues in natural water. For that, specific antibody will be labeled with gold nanoparticles and the immunoreaction would be detected in one reaction step. As a proof of concept, the immunoassay for the determination of chlorpyrifos will be developed. Different aspects of the immunoassay protocol have to be considered. First, gold nanoparticles of different sizes should be synthesized. Secondly, as particular aims, the following points should be evaluated: - the influence of nanoparticles size on the conjugation efficiency and assay sensitivity - the influence of sample volume on the reproducibility of the signal intensity - the influence of immunoreaction time on the sensitivity and reproducibility of the assay. Finally, the performance of the DVD microimmunoassay method should to be evaluated through the determination of organic pollutants residues in water samples. The immunoassay should achieve the detection limit established in E.U Water Framework Directive. es_ES
dc.format.extent 34 es_ES
dc.language Inglés es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Organic pollutants es_ES
dc.subject Water Framework Directive es_ES
dc.subject Gold nanoparticles es_ES
dc.subject compact disc technology es_ES
dc.subject Immunoassay es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.other Máster Universitario en Sensores para Aplicaciones Industriales-Màster Universitari en Sensors Per a Aplicacions Industrials es_ES
dc.title Gold-nanoparticle based immunoassay on a Digital Versatile Disk for the determination of priority pollutants residues in water es_ES
dc.type Tesis de máster es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny es_ES
dc.description.bibliographicCitation Dobosz, P. (2013). Gold-nanoparticle based immunoassay on a Digital Versatile Disk for the determination of priority pollutants residues in water. http://hdl.handle.net/10251/63122 es_ES
dc.description.accrualMethod Archivo delegado es_ES


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