- -

Relocation of the NIb gene in the tobacco etch potyvirus genome

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Relocation of the NIb gene in the tobacco etch potyvirus genome

Mostrar el registro sencillo del ítem

Ficheros en el ítem

Thumbnail
Nombre: - - Relocation of ...
Tamaño: 1.503Mb
Formato: PDF
Descripción: Versión editorial
Abrir
dc.contributor.author Majer, Eszter es_ES
dc.contributor.author Salvador, Zaira es_ES
dc.contributor.author Zwart, Mark Peter es_ES
dc.contributor.author Willemsen, Anouk es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2016-05-04T07:27:26Z
dc.date.available 2016-05-04T07:27:26Z
dc.date.issued 2014-04
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/63481
dc.description Supplemental material for this article may be found at http://dx.doi.org/10.1128/JVI.03336-13 es_ES
dc.description.abstract [EN] Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all possible intercistronic positions of the Tobacco etch virus (TEV) polyprotein. Only viruses with NIb at the amino terminus of the polyprotein or in between P1 and HC-Pro were viable in tobacco plants. es_ES
dc.description.sponsorship This research was supported by grant BIO2011-26741 from the Spanish Ministerio de Economia y Competitividad (MINECO) to J.-A.D., grant PROMETEO/2010/019 from Generalitat Valenciana to S. F. E. and J.-A.D., and grants BFU2012-30805 and 22371 from MINECO and the John Templeton Foundation, respectively, to S. F. E. E. M. was supported by a predoctoral fellowship (AP2012-3751) from the Spanish Ministerio de Educacion, Cultura y Deporte. M.P.Z. was supported by a Juan de la Cierva postdoctoral contract (JCI-2011-10379) from MINECO and a Rubicon grant from the Netherlands Organization for Scientific Research (www.nwo.nl). en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject VPG-PROTEINASE NIA es_ES
dc.subject HETEROLOGOUS PROTEINS es_ES
dc.subject VIRUS GENOME es_ES
dc.subject EXPRESSION es_ES
dc.subject STABILITY es_ES
dc.subject VECTOR es_ES
dc.subject PLANTS es_ES
dc.subject INSERTION es_ES
dc.subject MUTANTS es_ES
dc.subject PURIFICATION es_ES
dc.title Relocation of the NIb gene in the tobacco etch potyvirus genome es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.03336-13
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-26741/ES/PATOGENOS DE RNA DE PLANTAS: INTERACCION CON EL HUESPED Y DESARROLLO DE HERRAMIENTAS BIOTECNOLOGICAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GV//PROMETEO/2010/019/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-30805/ES/EVOLUTIONARY SYSTEMS VIROLOGY: EPISTASIS AND THE RUGGEDNESS OF ADAPTIVE LANDSCAPES, MUTATIONS IN REGULATORY SEQUENCES, AND THE HOST DETERMINANTS OF VIRAL FITNESS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//AP2012-3751/ES/AP2012-3751/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//JCI-2011-10379/ES/JCI-2011-10379/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/JTF//22371/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Majer, E.; Salvador, Z.; Zwart, MP.; Willemsen, A.; Elena Fito, SF.; Daros Arnau, JA. (2014). Relocation of the NIb gene in the tobacco etch potyvirus genome. Journal of Virology. (88):4586-4590. https://doi.org/10.1128/JVI.03336-13 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1128/JVI.03336-13 es_ES
dc.description.upvformatpinicio 4586 es_ES
dc.description.upvformatpfin 4590 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.issue 88 es_ES
dc.relation.senia 266231 es_ES
dc.identifier.eissn 1098-5514
dc.identifier.pmid 24453370 es_ES
dc.identifier.pmcid PMC3993717 en_EN
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder Netherlands Organization for Scientific Research
dc.contributor.funder John Templeton Foundation
dc.description.references Roossinck, M. J. (2011). The big unknown: plant virus biodiversity. Current Opinion in Virology, 1(1), 63-67. doi:10.1016/j.coviro.2011.05.022 es_ES
dc.description.references Chirico, N., Vianelli, A., & Belshaw, R. (2010). Why genes overlap in viruses. Proceedings of the Royal Society B: Biological Sciences, 277(1701), 3809-3817. doi:10.1098/rspb.2010.1052 es_ES
dc.description.references Belshaw, R., Pybus, O. G., & Rambaut, A. (2007). The evolution of genome compression and genomic novelty in RNA viruses. Genome Research, 17(10), 1496-1504. doi:10.1101/gr.6305707 es_ES
dc.description.references Nguyen, M., & Haenni, A.-L. (2003). Expression strategies of ambisense viruses. Virus Research, 93(2), 141-150. doi:10.1016/s0168-1702(03)00094-7 es_ES
dc.description.references Riechmann, J. L., Lain, S., & Garcia, J. A. (1992). Highlights and prospects of potyvirus molecular biology. Journal of General Virology, 73(1), 1-16. doi:10.1099/0022-1317-73-1-1 es_ES
dc.description.references Urcuqui-Inchima, S., Haenni, A.-L., & Bernardi, F. (2001). Potyvirus proteins: a wealth of functions. Virus Research, 74(1-2), 157-175. doi:10.1016/s0168-1702(01)00220-9 es_ES
dc.description.references Chung, B. Y.-W., Miller, W. A., Atkins, J. F., & Firth, A. E. (2008). An overlapping essential gene in the Potyviridae. Proceedings of the National Academy of Sciences, 105(15), 5897-5902. doi:10.1073/pnas.0800468105 es_ES
dc.description.references Li, X. H., & Carrington, J. C. (1995). Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells. Proceedings of the National Academy of Sciences, 92(2), 457-461. doi:10.1073/pnas.92.2.457 es_ES
dc.description.references Bedoya, L., Martínez, F., Rubio, L., & Daròs, J.-A. (2010). Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vector. Journal of Biotechnology, 150(2), 268-275. doi:10.1016/j.jbiotec.2010.08.006 es_ES
dc.description.references Bedoya, L. C., Martínez, F., Orzáez, D., & Daròs, J.-A. (2012). Visual Tracking of Plant Virus Infection and Movement Using a Reporter MYB Transcription Factor That Activates Anthocyanin Biosynthesis. Plant Physiology, 158(3), 1130-1138. doi:10.1104/pp.111.192922 es_ES
dc.description.references Majer, E., Daròs, J.-A., & Zwart, M. (2013). Stability and Fitness Impact of the Visually Discernible Rosea1 Marker in the Tobacco etch virus Genome. Viruses, 5(9), 2153-2168. doi:10.3390/v5092153 es_ES
dc.description.references Bedoya, L. C., & Daròs, J.-A. (2010). Stability of Tobacco etch virus infectious clones in plasmid vectors. Virus Research, 149(2), 234-240. doi:10.1016/j.virusres.2010.02.004 es_ES
dc.description.references Chen, C.-C., Chen, T.-C., Raja, J. A. J., Chang, C.-A., Chen, L.-W., Lin, S.-S., & Yeh, S.-D. (2007). Effectiveness and stability of heterologous proteins expressed in plants by Turnip mosaic virus vector at five different insertion sites. Virus Research, 130(1-2), 210-227. doi:10.1016/j.virusres.2007.06.014 es_ES
dc.description.references Merits, A., Runeberg-Roos, P., Rajamäki, M.-L., Puustinen, P., Mäkeläinen, K., Saarma, M., … Kekarainen, T. (2002). Proteolytic processing of potyviral proteins and polyprotein processing intermediates in insect and plant cells. Journal of General Virology, 83(5), 1211-1221. doi:10.1099/0022-1317-83-5-1211 es_ES
dc.description.references Parks, T. D., Howard, E. D., Wolpert, T. J., Arp, D. J., & Dougherty, W. G. (1995). Expression and Purification of a Recombinant Tobacco Etch Virus NIa Proteinase: Biochemical Analyses of the Full-Length and a Naturally Occurring Truncated Proteinase Form. Virology, 210(1), 194-201. doi:10.1006/viro.1995.1331 es_ES
dc.description.references KIM, D.-H., PARK, Y. S., KIM, S. S., LEW, J., NAM, H. G., & CHOI, K. Y. (1995). Expression, Purification, and Identification of a Novel Self-Cleavage Site of the NIa C-Terminal 27-kDa Protease of Turnip Mosaic Potyvirus C5. Virology, 213(2), 517-525. doi:10.1006/viro.1995.0024 es_ES
dc.description.references Dolja, V. V., McBride, H. J., & Carrington, J. C. (1992). Tagging of plant potyvirus replication and movement by insertion of beta-glucuronidase into the viral polyprotein. Proceedings of the National Academy of Sciences, 89(21), 10208-10212. doi:10.1073/pnas.89.21.10208 es_ES
dc.description.references Fernández-Fernández, M. R., Mouriño, M., Rivera, J., Rodríguez, F., Plana-Durán, J., & García, J. A. (2001). Protection of Rabbits against Rabbit Hemorrhagic Disease Virus by Immunization with the VP60 Protein Expressed in Plants with a Potyvirus-Based Vector. Virology, 280(2), 283-291. doi:10.1006/viro.2000.0762 es_ES
dc.description.references Sánchez, F., Sáez, M., Lunello, P., & Ponz, F. (2013). Plant viral elongated nanoparticles modified for log-increases of foreign peptide immunogenicity and specific antibody detection. Journal of Biotechnology, 168(4), 409-415. doi:10.1016/j.jbiotec.2013.09.002 es_ES
dc.description.references Masuta, C., Yamana, T., Tacahashi, Y., Uyeda, I., Sato, M., Ueda, S., & Matsumura, T. (2000). Development of clover yellow vein virus as an efficient, stable gene-expression system for legume species. The Plant Journal, 23(4), 539-546. doi:10.1046/j.1365-313x.2000.00795.x es_ES
dc.description.references Beauchemin, C., Bougie, V., & Laliberté, J.-F. (2005). Simultaneous production of two foreign proteins from a potyvirus-based vector. Virus Research, 112(1-2), 1-8. doi:10.1016/j.virusres.2005.03.001 es_ES
dc.description.references Kelloniemi, J., Mäkinen, K., & Valkonen, J. P. T. (2008). Three heterologous proteins simultaneously expressed from a chimeric potyvirus: Infectivity, stability and the correlation of genome and virion lengths. Virus Research, 135(2), 282-291. doi:10.1016/j.virusres.2008.04.006 es_ES
dc.description.references Whitham, S. A., Yamamoto, M. L., & Carrington, J. C. (1999). Selectable viruses and altered susceptibility mutants in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 96(2), 772-777. doi:10.1073/pnas.96.2.772 es_ES
dc.description.references Dolja, V. V., Hong, J., Keller, K. E., Martin, R. R., & Peremyslov, V. V. (1997). Suppression of Potyvirus Infection by Coexpressed Closterovirus Protein. Virology, 234(2), 243-252. doi:10.1006/viro.1997.8660 es_ES
dc.description.references Dietrich, C. (2003). Fluorescent labelling reveals spatial separation of potyvirus populations in mixed infected Nicotiana benthamiana plants. Journal of General Virology, 84(10), 2871-2876. doi:10.1099/vir.0.19245-0 es_ES
dc.description.references Rajamäki, M.-L., Kelloniemi, J., Alminaite, A., Kekarainen, T., Rabenstein, F., & Valkonen, J. P. T. (2005). A novel insertion site inside the potyvirus P1 cistron allows expression of heterologous proteins and suggests some P1 functions. Virology, 342(1), 88-101. doi:10.1016/j.virol.2005.07.019 es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem