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Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection

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Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection

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dc.contributor.author Martínez, Fernando es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2016-05-04T07:38:01Z
dc.date.available 2016-05-04T07:38:01Z
dc.date.issued 2014-09
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/63483
dc.description.abstract [EN] The genus Potyvirus comprises a large group of positive-strand RNA plant viruses whose genome encodes a large polyprotein processed by three viral proteinases. P1 protein, the most amino-terminal product of the polyprotein, is an accessory factor stimulating viral genome amplification whose role during infection is not well understood. We infected plants with Tobacco etch virus (TEV; genus Potyvirus) clones in which P1 was tagged with a fluorescent protein to track its expression and subcellular localization or with an affinity tag to identify host proteins involved in complexes in which P1 also takes part during infection. Our results showed that TEV P1 exclusively accumulates in infected cells at an early stage of infection and that the protein displays a dynamic subcellular localization, trafficking in and out of the nucleus and nucleolus during infection. Inside the nucleolus, P1 particularly targets the dense granular component. Consistently, we found functional nucleolar localization and nuclear export signals in TEV P1 sequence. Our results also indicated that TEV P1 physically interacts with the host 80S cytoplasmic ribosomes and specifically binds to the 60S ribosomal subunits during infection. In vitro translation assays of reporter proteins suggested that TEV P1 stimulates protein translation, particularly when driven from the TEV internal ribosome entry site. These in vitro assays also suggested that TEV helper-component proteinase (HC-Pro) inhibits protein translation. Based on these findings, we propose that TEV P1 stimulates translation of viral proteins in infected cells. IMPORTANCE In this work, we researched the role during infection of tobacco etch virus P1 protease. P1 is the most mysterious protein of potyviruses, a relevant group of RNA viruses infecting plants. Our experiments showed that the viral P1 protein exclusively accumulates in infected cells at an early stage of infection and moves in and out of the nucleus of infected cells, particularly targeting the nucleolus. Our experiments also showed that P1 protein binds host ribosomes during infection. Based on these findings and other in vitro experiments we propose that P1 protein stimulates translation of viral proteins during infection es_ES
dc.description.sponsorship This work was supported by grant BIO2011-26741 from the Spanish Ministerio de Economia y Competitividad. F.M. was the recipient of a predoctoral fellowship from Universidad Politecnica de Valencia. en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject HELPER-COMPONENT PROTEINASE es_ES
dc.subject CAP-INDEPENDENT TRANSLATION es_ES
dc.subject CAULIFLOWER-MOSAIC-VIRUS es_ES
dc.subject DEPENDENT RNA-POLYMERASE es_ES
dc.subject FAMILY POTYVIRIDAE es_ES
dc.subject COAT PROTEIN es_ES
dc.subject ARABIDOPSIS-THALIANA es_ES
dc.subject NUCLEAR EXPORT es_ES
dc.subject N-TERMINUS es_ES
dc.subject 5' LEADER es_ES
dc.title Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.00928-14
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-26741/ES/PATOGENOS DE RNA DE PLANTAS: INTERACCION CON EL HUESPED Y DESARROLLO DE HERRAMIENTAS BIOTECNOLOGICAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Martínez, F.; Daros Arnau, JA. (2014). Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection. Journal of Virology. 88(18):10725-10737. https://doi.org/10.1128/JVI.00928-14 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1128/JVI.00928-14 es_ES
dc.description.upvformatpinicio 10725 es_ES
dc.description.upvformatpfin 10737 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 88 es_ES
dc.description.issue 18 es_ES
dc.relation.senia 285408 es_ES
dc.identifier.eissn 1098-5514
dc.identifier.pmcid PMC4178839 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
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