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Comparative transcriptional profiling analysis of developing melon (Cucumis melo L.) fruit from climacteric and non-climacteric varieties

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Comparative transcriptional profiling analysis of developing melon (Cucumis melo L.) fruit from climacteric and non-climacteric varieties

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dc.contributor.author Saladie, Montserrat es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Michael A. Phillips es_ES
dc.contributor.author Rodriguez Concepcion, Manuel es_ES
dc.contributor.author Larrigaudière, Christian es_ES
dc.contributor.author Gibon, Yves es_ES
dc.contributor.author Stitt, Mark es_ES
dc.contributor.author Lunn, John Edward es_ES
dc.contributor.author Garcia Mas, Jordi es_ES
dc.date.accessioned 2016-05-10T10:02:29Z
dc.date.available 2016-05-10T10:02:29Z
dc.date.issued 2015-06-09
dc.identifier.issn 1471-2164
dc.identifier.uri http://hdl.handle.net/10251/63839
dc.description.abstract [EN] Background: In climacteric fruit-bearing species, the onset of fruit ripening is marked by a transient rise in respiration rate and autocatalytic ethylene production, followed by rapid deterioration in fruit quality. In non-climacteric species, there is no increase in respiration or ethylene production at the beginning or during fruit ripening. Melon is unusual in having climacteric and non-climacteric varieties, providing an interesting model system to compare both ripening types. Transcriptomic analysis of developing melon fruits from Védrantais and Dulce (climacteric) and Piel de sapo and PI 161375 (non-climacteric) varieties was performed to understand the molecular mechanisms that differentiate the two fruit ripening types. Results: Fruits were harvested at 15, 25, 35 days after pollination and at fruit maturity. Transcript profiling was performed using an oligo-based microarray with 75 K probes. Genes linked to characteristic traits of fruit ripening were differentially expressed between climacteric and non-climacteric types, as well as several transcription factor genes and genes encoding enzymes involved in sucrose catabolism. The expression patterns of some genes in PI 161375 fruits were either intermediate between. Piel de sapo and the climacteric varieties, or more similar to the latter. PI 161375 fruits also accumulated some carotenoids, a characteristic trait of climacteric varieties. Conclusions: Simultaneous changes in transcript abundance indicate that there is coordinated reprogramming of gene expression during fruit development and at the onset of ripening in both climacteric and non-climacteric fruits. The expression patterns of genes related to ethylene metabolism, carotenoid accumulation, cell wall integrity and transcriptional regulation varied between genotypes and was consistent with the differences in their fruit ripening characteristics. There were differences between climacteric and non-climacteric varieties in the expression of genes related to sugar metabolism suggesting that they may be potential determinants of sucrose content and post-harvest stability of sucrose levels in fruit. Several transcription factor genes were also identified that were differentially expressed in both types, implicating them in regulation of ripening behaviour. The intermediate nature of PI 161375 suggested that classification of melon fruit ripening behaviour into just two distinct types is an over-simplification, and that in reality there is a continuous spectrum of fruit ripening behaviour es_ES
dc.description.sponsorship We wish to thank Marta Casado for assistance with qRT-PCR analysis, Anna Orozco and Rosa Rodriguez for help with carotenoid measurements, Kostas Alexiou for uploading the microarray data at GEO and Walter Sanseverino for obtaining the correspondence of the microarray contigs with the melon annotated genes. We thank Beatrice Encke and Nicole Krohn for technical help with the sugar analysis. We also thank Laura Pascual for a critical reading of the manuscript. MSa was supported by a JAE-Doc grant from the Spanish Ministry of Science. This work was supported by the Spanish Ministry of Science and Innovation (MICINN project GEN2006-27773-C2-1-E to JGM) and the German Federal Ministry of Education and Research (BMBF project 0313987 to MSt) within the framework of the EU Framework 6 ERA-NET Plant Genomics programme. en_EN
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation.ispartof BMC Genomics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Carotenoids es_ES
dc.subject Fruit ripening es_ES
dc.subject Ethylene es_ES
dc.subject Fruit development es_ES
dc.subject Microarray es_ES
dc.subject Sucrose es_ES
dc.subject.classification GENETICA es_ES
dc.title Comparative transcriptional profiling analysis of developing melon (Cucumis melo L.) fruit from climacteric and non-climacteric varieties es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12864-015-1649-3
dc.relation.projectID info:eu-repo/grantAgreement/MEC//GEN2006-27773-C2-1-E/ES/MELRIP: UNDERSTANDING THE CLIMACTERIC VS NON-CLIMACTERIC FRUIT RIPENING MECHANISMS IN MELON USING TRANSCRIPTOMIC, METABOLOMIC AND REVERSE GENETIC APPROACHES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/BMBF//0313987/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Saladie, M.; Cañizares Sales, J.; Michael A. Phillips; Rodriguez Concepcion, M.; Larrigaudière, C.; Gibon, Y.; Stitt, M.... (2015). Comparative transcriptional profiling analysis of developing melon (Cucumis melo L.) fruit from climacteric and non-climacteric varieties. BMC Genomics. 16(440):1-20. https://doi.org/10.1186/s12864-015-1649-3 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1186/s12864-015-1649-3 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 20 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 440 es_ES
dc.relation.senia 292292 es_ES
dc.identifier.pmid 26054931 en_EN
dc.identifier.pmcid PMC4460886 en_EN
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.contributor.funder Bundesministerium für Bildung und Forschung, Alemania es_ES
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