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Cryopreservation of Bituminaria bituminosa varieties and hybrids

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Cryopreservation of Bituminaria bituminosa varieties and hybrids

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dc.contributor.author Gisbert Domenech, María Carmen es_ES
dc.contributor.author Dabauza, Mercedes es_ES
dc.contributor.author Correal, Enrique es_ES
dc.contributor.author Swennen, Rony es_ES
dc.contributor.author Panis, Bart es_ES
dc.date.accessioned 2016-05-11T10:09:59Z
dc.date.available 2016-05-11T10:09:59Z
dc.date.issued 2015-10
dc.identifier.issn 0011-2240
dc.identifier.uri http://hdl.handle.net/10251/63894
dc.description.abstract [EN] Bituminaria bituminosa (L.) C.H. Stirton is a drought tolerant, perennial legume pasture species and a source of pharmaceutical compounds. Bituminaria breeding programs aim to develop and conserve hybrids with desirable traits such as high forage quality, tolerance to biotic or abiotic stresses, and high contents of furanocoumarins. In this work we present a cryopreservation study of different B. bituminosa accessions: two varieties and eight intervarietal hybrids resulting from crosses between the three botanical varieties: var. bituminosa, var. crassiuscula, and var. albomarginata. No previous work on cryopreservation of Bituminaria species has been reported. We applied the ultra-fast cooling method, using droplet vitrification on aluminum foil strips. First, we investigated the PVS2 toxicity and cryopreservation damage in two genotypes, comparing three PVS2 treatments and two culture media. An incubation of 30 min in PVS2 resulted in regeneration rates after cryopreservation higher than 80%. The MS medium was selected for optimal meristem outgrowth, in order to avoid the prominent callus formation that was observed in the presence of BAP. These conditions were subsequently used to cryopreserve eight other genotypes. The results were highly variable; 45 days after cryopreservation, survival ranged between 22% and 98% while regeneration ranged between 0% and 96%, depending on the accession. A significant and positive correlation was observed between survival and regeneration. At 90 days post culture plantlets could be recovered from cryopreserved explants of all genotypes. This study shows that the droplet vitrification method is promising for the cryopreservation of eight of the 10 genotypes assayed and the method can thus be applied to develop a cryobank of B. bituminosa. 2015 Elsevier Inc. All rights reserved. es_ES
dc.description.sponsorship Projects and institutions that funded this research are in acknowledgments. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//BFU2010-19599/ES/ESTUDIO DE LA BIOSINTESIS REGLACION Y ACTIVIDAD TERAPEUTICA DE LAS FURANOCUMARINAS DE BITUMINARIA BITUMINOSA/ es_ES
dc.relation IMIDA es_ES
dc.relation European Social Fund (ESF) es_ES
dc.relation COMAV in UPV es_ES
dc.relation Universitat Politecnica de Valencia (UPV) PAID-00-10 es_ES
dc.relation.ispartof Cryobiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Droplet vitrification es_ES
dc.subject Tedera es_ES
dc.subject var. Crassiuscula es_ES
dc.subject var. albomarginata es_ES
dc.subject Psoralea bituminosa es_ES
dc.subject Conservación es_ES
dc.subject Recursos genéticos es_ES
dc.subject.classification GENETICA es_ES
dc.title Cryopreservation of Bituminaria bituminosa varieties and hybrids es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.cryobiol.2015.07.011
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Gisbert Domenech, MC.; Dabauza, M.; Correal, E.; Swennen, R.; Panis, B. (2015). Cryopreservation of Bituminaria bituminosa varieties and hybrids. Cryobiology. 71(2):279-285. https://doi.org/10.1016/j.cryobiol.2015.07.011 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.cryobiol.2015.07.011 es_ES
dc.description.upvformatpinicio 279 es_ES
dc.description.upvformatpfin 285 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 71 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 303069 es_ES
dc.identifier.eissn 1090-2392
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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