- -

Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms

Show simple item record

Files in this item

dc.contributor.author Adell Sales, Elisa es_ES
dc.contributor.author Moset, Veronica es_ES
dc.contributor.author Zhao, Yang es_ES
dc.contributor.author Jiménez Belenguer, Ana Isabel es_ES
dc.contributor.author Cerisuelo, Alba es_ES
dc.contributor.author Cambra López, María es_ES
dc.date.accessioned 2016-05-16T13:58:10Z
dc.date.available 2016-05-16T13:58:10Z
dc.date.issued 2014
dc.identifier.issn 1232-1966
dc.identifier.uri http://hdl.handle.net/10251/64122
dc.description.abstract Sampling techniques to detect airborne Salmonella species (spp.) in two pilot scale broiler houses were compared. Broilers were inoculated at seven days of age with a marked strain of Salmonella enteritidis. The rearing cycle lasted 42 days during the summer. Airborne Salmonella spp. were sampled weekly using impaction, gravitational settling, and impingement techniques. Additionally,Salmonella spp. were sampled on feeders, drinkers, walls, and in the litter. Environmental conditions (temperature, relative humidity, and airborne particulate matter (PM) concentration) were monitored during the rearing cycle. The presence of Salmonella spp. was determined by culture-dependent and molecular methods. No cultivable Salmonella spp. were recovered from the poultry houses' surfaces, the litter, or the air before inoculation. After inoculation, cultivable Salmonella spp. were recovered from the surfaces and in the litter. Airborne cultivable Salmonella spp. were detected using impaction and gravitational settling one or two weeks after the detection of Salmonella spp. in the litter. No cultivable Salmonella spp. were recovered using impingement based on culture-dependent techniques. At low airborne concentrations, the use of impingement for the quantification or detection of cultivable airborne Salmonella spp. is not recommended. In these cases, a combination of culture-dependent and culture-independent methods is recommended. These data are valuable to improve current measures to control the transmission of pathogens in livestock environments and for optimising the sampling and detection of airborne Salmonella spp. in practical conditions. es_ES
dc.description.sponsorship The research was funded by the Spanish Ministry of Science and Innovation (Project No. GASFARM-2 AGL2008-04125, Madrid). The authors express their thanks to the Centro de Tecnologia Animal (IVIA, Spain) and its staff for providing the broiler installations and collaborating during this study. We are also grateful to the Livestock Research Group of Wageningen UR, The Netherlands, for their equipment support. The authors also wish to thank the Campus de Excelencia Internacional of the Universitat Politecnica de Valencia, Spain.
dc.language Inglés es_ES
dc.publisher Institute of Agricultural Medicine in Lublin es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//AGL2008-04125/ES/MEJORA DE LA ESTIMACION DE EMISIONES DE GASES CONTAMINANTES Y DE PARTICULAS, Y ESTUDIO DE ALTERNATIVAS DE SU REDUCCION EN GRANJAS INTENSIVAS AVICOLAS Y CUNICOLAS/ es_ES
dc.relation.ispartof Annals of Agricultural and Environmental Medicine es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Airborne transmission es_ES
dc.subject Air quality es_ES
dc.subject Bioaerosol es_ES
dc.subject Broiler housing es_ES
dc.subject Particulate matter es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms es_ES
dc.type Artículo es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro Avanzado de Microbiología de Alimentos - Centre Avançat de Microbiologia d'Aliments es_ES
dc.description.bibliographicCitation Adell Sales, E.; Moset, V.; Zhao, Y.; Jiménez Belenguer, AI.; Cerisuelo, A.; Cambra López, M. (2014). Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms. Annals of Agricultural and Environmental Medicine. 21(1):15-24. http://hdl.handle.net/10251/64122 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://www.aaem.pl/Archive es_ES
dc.description.upvformatpinicio 15 es_ES
dc.description.upvformatpfin 24 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 21 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 259886 es_ES
dc.identifier.eissn 1898-2263
dc.identifier.pmid 24742034
dc.contributor.funder Ministerio de Ciencia e Innovación


This item appears in the following Collection(s)

Show simple item record