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Culture of human bone marrow-derived mesenchymal stem cells on of poly(L-lactic acid) scaffolds: potential application for the tissue engineering of cartilage

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Culture of human bone marrow-derived mesenchymal stem cells on of poly(L-lactic acid) scaffolds: potential application for the tissue engineering of cartilage

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dc.contributor.author Izal, Iñigo es_ES
dc.contributor.author Aranda, Pablo es_ES
dc.contributor.author Sanz Ramos, Patricia es_ES
dc.contributor.author Ripalda, Purificacion es_ES
dc.contributor.author Mora, Gonzalo es_ES
dc.contributor.author Granero Molto, Froilan es_ES
dc.contributor.author Deplaine, Harmony es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.contributor.author Acosta, Victor es_ES
dc.contributor.author Ochoa, Ignacio es_ES
dc.contributor.author García Aznar, Manuel es_ES
dc.contributor.author Andreu, Enrique J. es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.contributor.author Doblare Castellano, Manuel es_ES
dc.contributor.author Prosper, Felipe es_ES
dc.date.accessioned 2016-05-17T08:21:46Z
dc.date.available 2016-05-17T08:21:46Z
dc.date.issued 2013-08
dc.identifier.issn 0942-2056
dc.identifier.uri http://hdl.handle.net/10251/64199
dc.description.abstract Due to the attractive properties of poly(l-lactic acid) (PLLA) for tissue engineering, the aim was to determine the growth and differentiation capacity of mesenchymal stromal cells (MSCs) in PLLA scaffolds and their potential use in the treatment of cartilage diseases. MSCs were cultured in PLLA films and thin porous membranes to study adherence and proliferation. Permeability and porosity were determined for the different scaffolds employed. The optimal conditions for cell seeding were first determined, as well as cell density and distribution inside the PLLA. Scaffolds were then maintained in expansion or chondrogenic differentiation media for 21 days. Apoptosis, proliferation and chondrogenic differentiation was assessed after 21 days in culture by immunohistochemistry. Mechanical characteristics of scaffolds were determined before and after cell seeding. MSCs uniformly adhered to PLLA films as well as to porous membranes. Proliferation was detected only in monolayers of pure PLLA, but was no longer detected after 10 days. Mechanical characterization of PLLA scaffolds showed differences in the apparent compression elastic modulus for the two sizes used. After determining high efficiencies of seeding, the production of extracellular matrix (ECM) was determined and contained aggrecan and collagens type I and X. ECM produced by the cells induced a twofold increase in the apparent elastic modulus of the composite. Biocompatible PLLA scaffolds have been developed that can be efficiently loaded with MSCs. The scaffold supports chondrogenic differentiation and ECM deposition that improves the mechanics of the scaffold. Although this improvement does not met the expectations of a hyaline-like cartilage ECM, in part due to the lack of a mechanical stimulation, their potential use in the treatment of cartilage pathologies encourages to improve the mechanical component. es_ES
dc.description.sponsorship This work has been supported by the Spanish Ministry of Science and Innovation DPI2010-20399-C04-00 project and Instituto de Salud Carlos III RETIC RD06/0014. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag es_ES
dc.relation.ispartof Knee Surgery, Sports Traumatology, Arthroscopy es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Tissue engineering es_ES
dc.subject Chondrocyte differentiation es_ES
dc.subject PLLA scaffolds es_ES
dc.subject Mesenchymal stem cells es_ES
dc.subject.classification INGENIERIA MECANICA es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Culture of human bone marrow-derived mesenchymal stem cells on of poly(L-lactic acid) scaffolds: potential application for the tissue engineering of cartilage es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00167-012-2148-6
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//DPI2010-20399-C04-04/ES/DISEÑO, CONSTRUCCION Y VALIDACION DE UNA PLATAFORMA BIOMIMETICA PARA LA EVALUACION FUNCIONAL Y OPTIMIZACION DE CONSTRUCTOS DE INGENIERIA TISULAR PARA LA REPARACION DE CARTILAG/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//DPI2010-20399-C04-03/ES/DISEÑO Y FABRICACION DE UNA PLATAFORMA BIOMIMETICA TIPO SCAFFOLD%2FSOPORTE PARA LA REGENERACION DEL CARTILAGO ARTICULAR/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MSC//RD06%2F0014/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//DPI2010-20399-C04-01/ES/DISEÑO, CONSTRUCCION Y VALIDACION DE UNA PLATAFORMA BIOMIMETICA PARA LA EVALUACION FUNCIONAL Y OPTIMIZACION DE CONSTRUCTOS DE INGENIERIA TISULAR DE CARTILAGO ARTICULAR/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.description.bibliographicCitation Izal, I.; Aranda, P.; Sanz Ramos, P.; Ripalda, P.; Mora, G.; Granero Molto, F.; Deplaine, H.... (2013). Culture of human bone marrow-derived mesenchymal stem cells on of poly(L-lactic acid) scaffolds: potential application for the tissue engineering of cartilage. Knee Surgery, Sports Traumatology, Arthroscopy. 21(8):1737-1750. https://doi.org/10.1007/s00167-012-2148-6 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s00167-012-2148-6 es_ES
dc.description.upvformatpinicio 1737 es_ES
dc.description.upvformatpfin 1750 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 21 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 260189 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Sanidad y Consumo es_ES
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