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Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance

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Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance

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dc.contributor.author González Guzmán, Miguel es_ES
dc.contributor.author Rodriguez, Lesia es_ES
dc.contributor.author Lorenzo Orts, Laura es_ES
dc.contributor.author Pons Puig, Clara es_ES
dc.contributor.author Sarrion-Perdigones, Alejandro es_ES
dc.contributor.author Fernandez, Maria A es_ES
dc.contributor.author Peirats Llobet, Marta es_ES
dc.contributor.author Forment Millet, José Javier . es_ES
dc.contributor.author Moreno Alvero, María es_ES
dc.contributor.author Cutler, Sean R. es_ES
dc.contributor.author Albert, Armando es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís
dc.date.accessioned 2016-05-19T10:58:14Z
dc.date.available 2016-05-19T10:58:14Z
dc.date.issued 2014-08
dc.identifier.issn 0022-0957
dc.identifier.uri http://hdl.handle.net/10251/64399
dc.description This article contains supplementary data at: http://jxb.oxfordjournals.org/content/65/15/4451/suppl/DC1 © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology es_ES
dc.description.abstract [EN] Abscisic acid (ABA) plays a crucial role in the plant’s response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly the generation of new varieties with improved water use efciency and drought tolerance. Different studies have shown the potential applications of Arabidopsis PYR/PYL/ RCAR ABA receptors to enhance plant drought resistance. Consequently the functional characterization of orthologous genes in crops holds promise for agriculture. The full set of tomato (Solanum lycopersicum) PYR/PYL/RCAR ABA receptors have been identied here. From the 15 putative tomato ABA receptors, 14 of them could be grouped in three subfamilies that correlated well with corresponding Arabidopsis subfamilies. High levels of expression of PYR/ PYL/RCAR genes was found in tomato root, and some genes showed predominant expression in leaf and fruit tissues. Functional characterization of tomato receptors was performed through interaction assays with Arabidopsis and tomato clade A protein phosphatase type 2Cs (PP2Cs) as well as phosphatase inhibition studies. Tomato receptors were able to inhibit the activity of clade A PP2Cs differentially in an ABA-dependent manner, and at least three receptors were sensitive to the ABA agonist quinabactin, which inhibited tomato seed germination. Indeed, the chemical activation of ABA signalling induced by quinabactin was able to activate stress-responsive genes. Both dimeric and monomeric tomato receptors were functional in Arabidopsis plant cells, but only overexpression of monomeric-type receptors conferred enhanced drought resistance. In summary, gene expression analyses, and chemical and transgenic approaches revealed distinct properties of tomato PYR/PYL/RCAR ABA receptors that might have biotechnological implications. es_ES
dc.description.sponsorship This work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas (grants BIO2011-23446 to PLR, BFU2011-25384 to AA, and Fontagro and COST1106 for networking activities to AG); fellowships to LR and MP; and a Juan de la Cierva contract to MGG. Cristina Martinez-Andujar and the Bioinformatics Core Service of the IBMCP are acknowledged for the SlEF1a marker and help in bioinformatics analyses, respectively. en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation Ministerio de Ciencia e Innovacion es_ES
dc.relation Fondo Europeo de Desarrollo Regional es_ES
dc.relation Consejo Superior de Investigaciones Cientificas BIO2011-23446 es_ES
dc.relation Consejo Superior de Investigaciones Cientificas BFU2011-25384 es_ES
dc.relation Consejo Superior de Investigaciones Cientificas COST1106 es_ES
dc.relation Juan de la Cierva es_ES
dc.relation.ispartof Journal of Experimental Botany es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Abscisic acid (ABA) es_ES
dc.subject drought resistance es_ES
dc.subject tomato ABA receptor es_ES
dc.subject tomato clade A PP2C es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/jxb/eru219
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gonzalez Guzman, M.; Rodriguez, L.; Lorenzo Orts, L.; Pons Puig, C.; Sarrion-Perdigones, A.; Fernandez, MA.; Peirats Llobet, M.... (2014). Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance. Journal of Experimental Botany. 65(15):4451-4464. doi:10.1093/jxb/eru219 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1093/jxb/eru219 es_ES
dc.description.upvformatpinicio 4451 es_ES
dc.description.upvformatpfin 4464 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 65 es_ES
dc.description.issue 15 es_ES
dc.relation.senia 284262 es_ES
dc.identifier.eissn 1460-2431
dc.identifier.pmid 24863435 en_EN
dc.identifier.pmcid PMC4112642 en_EN


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