Tamarit López, J.; Morais Ezquerro, SB.; Puchades Pla, R.; Maquieira Catala, Á. (2011). Direct hapten-linked multiplexed immunoassays on polycarbonate surface. Biosensors and Bioelectronics. 26(5):2694-2698. https://doi.org/10.1016/j.bios.2010.08.066
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/64608
Title:
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Direct hapten-linked multiplexed immunoassays on polycarbonate surface
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Author:
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Tamarit López, Jesús
Morais Ezquerro, Sergi Beñat
Puchades Pla, Rosa
Maquieira Catala, Ángel
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UPV Unit:
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Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic
Universitat Politècnica de València. Departamento de Química - Departament de Química
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Issued date:
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Abstract:
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Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are ...[+]
Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are directly linked to the aminated polycarbonate surface through carbodiimide/succinimide coupling. The modified DVD surface maintained its physical and optical properties. Multiplexed assay reached detection limits down to 0.1¿g/L for chlorpyrifos, 2,4,5-trichlorophenoxypropionic acid, sulfathiazole and sulfasalazine and down to 1.0¿g/L for fenthion and malathion. This approach presents advantages such as the improvement in sensitivity in comparison to protein-hapten conjugate format for all the studied analytes and the absence of cross-interference effects, allowing high throughput multianalysis on the same surface. Also, a comparison of the performance of two sensing strategies indicated that DVD disc and drive approach turned out in a simpler mode, the assays being more reproducible and with higher signal to noise ratios. © 2010 Elsevier B.V.
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Subjects:
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Digital versatile disc
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Direct hapten-linked immunoassay
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Microarray
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Multiplexed analysis
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Polycarbonate
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Analytes
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Chlorpyrifos
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Detection limits
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Environmental concerns
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Fenthion
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High throughput
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Interference effects
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Multianalysis
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Proof of concept
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Sulfathiazole
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Assays
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Data storage equipment
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Immunology
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Optical properties
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Plastic products
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Signal to noise ratio
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Videodisks
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Multiplexing
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Cyanamide
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Hapten
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Malathion
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Salazosulfapyridine
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Succinimide
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Water
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Article
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Chemical modification
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Compact disk
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Controlled study
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Digital versatile device
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Direct hapten linked multiplexed immunoassay
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Hapten carrier complex
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High throughput screening
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Immunoassay
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Immunocytochemistry
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Physical chemistry
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Process development
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Quantitative analysis
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Sensitivity analysis
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Signal noise ratio
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Surface property
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Compact Disks
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Environmental Monitoring
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Environmental Pollutants
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Equipment Design
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Equipment Failure Analysis
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Haptens
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Polycarboxylate Cement
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Surface Properties
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Copyrigths:
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Cerrado |
Source:
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Biosensors and Bioelectronics. (issn:
0956-5663
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DOI:
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10.1016/j.bios.2010.08.066
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Publisher:
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Elsevier
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Publisher version:
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http://dx.doi.org/10.1016/j.bios.2010.08.066
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Project ID:
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info:eu-repo/grantAgreement/MEC//CTQ2007-64735/ES/ESTRATEGIAS PARA EL DESARROLLO DE MICROSISTEMAS ANALITICOS DE BARRIDO BASADOS EN RECEPTORES BIOMOLECULARES Y SU APLICACION A LAS CIENCIAS DE LA VIDA/
info:eu-repo/grantAgreement/GVA//ACOMP%2F2009%2F650/
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Thanks:
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This research was funded through FEDER projects CTQ2007-64735/BQU (CICYT, Spain) and ACOMPGVA-2009/650 (GVA, Spain). The Spanish MEC provided J.T.-L. with a grant for his PhD studies.
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Type:
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Artículo
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