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The regulation of aromatase and androgen receptor expression during gonad development in male and female European eel

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The regulation of aromatase and androgen receptor expression during gonad development in male and female European eel

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dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Mazzeo, Ilaria es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Hildahl, Jon es_ES
dc.contributor.author Nourizadeh-Lillabadi, Rasoul es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Weltzien, Finn Arne es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.date.accessioned 2016-05-26T09:24:47Z
dc.date.available 2016-05-26T09:24:47Z
dc.date.issued 2014-06
dc.identifier.issn 0936-6768
dc.identifier.uri http://hdl.handle.net/10251/64758
dc.description.abstract [EN] This research investigated the regulation of aromatase and androgen receptor gene expression in the brain–pituitary– gonad (BPG) axis of male and female European eels (Anguilla anguilla) during induced sexual maturation. Complete A. anguilla aromatase (aa-cyp19a1) and partial androgen receptor a and b (aa-ara and aa-arb) sequences were isolated, and qPCR assays were validated and used for quantification of transcript levels for these three genes. Expression levels of the genes varied with sex, tissue and stage of maturation. aa-arb was expressed at higher levels than aa-ara in the pituitary and gonad in both sexes, suggesting aa-arb is the physiologically most important androgen receptor in these tissues. In the female brain, a decrease in aa-ara and an increase in aa-cyp19a1 were observed at the vitellogenic stage. In contrast, a progressive increase in all three genes was observed in the pituitary and ovaries throughout gonadal development, with aa-arb and aa-cyp19a1 reaching significantly higher levels at the vitellogenic stage. In the male pituitary, a decrease in aa-arb and an increase in aa-cyp19a1 were observed at the beginning of spermatogenesis, and thereafter remained low and high, respectively. In the testis, the transcript levels of androgen receptors and aa-cyp19a1 were higher during the early stages of spermatogenesis and decreased thereafter. These sex-dependent differences in the regulation of the expression of aa-ara, aa-arb and cyp19a1 are discussed in relation to the role of androgens and their potential aromatization in the European eel during gonadal maturation es_ES
dc.description.sponsorship This work was funded by the European Community's 7th Framework Programme under the Theme 2 'Food, Agriculture and Fisheries, and Biotechnology', grant agreement no 245257 (PRO-EEL). D. S. P. received a postdoc grant from UPV (CEI-01-10), a mobility grant from UPV (PAID-00-11) and has also been supported by a contract cofinanced by MICINN and UPV (PTA2011-4948-I). V. G. and I. M. received predoctoral grants from the Spanish Ministry of Science and Innovation (MICINN) and Generalitat Valenciana, respectively. F.-A. W. received funding from the Norwegian School of Veterinary Science. The fish farm Valenciana de Acuicultura, S. A. supplied the male eels used in the experiments. The English revision was carried out by Professor Lucy Robertson (Lucy Robertson Writing Services, Norway). en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Reproduction in Domestic Animals es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject cyp19a1 es_ES
dc.subject Brain-pituitary-gonad axis es_ES
dc.subject Reproduction es_ES
dc.subject Testis es_ES
dc.subject Ovary es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title The regulation of aromatase and androgen receptor expression during gonad development in male and female European eel es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/rda.12321
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/245257/EU/Reproduction of European Eel: Towards a Self-sustained Aquaculture/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//CE-01-10/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-00-11/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//PTA2011-4948-I/ES/PTA2011-4948-I/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Peñaranda, D.; Mazzeo, I.; Gallego Albiach, V.; Hildahl, J.; Nourizadeh-Lillabadi, R.; Pérez Igualada, LM.; Weltzien, FA.... (2014). The regulation of aromatase and androgen receptor expression during gonad development in male and female European eel. Reproduction in Domestic Animals. 49(3):512-521. https://doi.org/10.1111/rda.12321 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/rda.12321 es_ES
dc.description.upvformatpinicio 512 es_ES
dc.description.upvformatpfin 521 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 49 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 268943 es_ES
dc.identifier.eissn 1439-0531
dc.contributor.funder European Commission
dc.contributor.funder Universitat Politècnica de València
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Generalitat Valenciana
dc.description.references Aroua, S., Weltzien, F.-A., Belle, N. L., & Dufour, S. (2007). Development of real-time RT-PCR assays for eel gonadotropins and their application to the comparison of in vivo and in vitro effects of sex steroids. General and Comparative Endocrinology, 153(1-3), 333-343. doi:10.1016/j.ygcen.2007.02.027 es_ES
dc.description.references Blázquez, M., & Piferrer, F. (2004). Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax). Molecular and Cellular Endocrinology, 219(1-2), 83-94. doi:10.1016/j.mce.2004.01.006 es_ES
dc.description.references Blázquez, M., & Piferrer, F. (2005). Sea bass (Dicentrarchus labrax) androgen receptor: cDNA cloning, tissue-specific expression, and mRNA levels during early development and sex differentiation. Molecular and Cellular Endocrinology, 237(1-2), 37-48. doi:10.1016/j.mce.2005.04.001 es_ES
dc.description.references Borg, B. (1994). Androgens in teleost fishes. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 109(3), 219-245. doi:10.1016/0742-8413(94)00063-g es_ES
dc.description.references Chang, X. T., Kobayashi, T., Kajiura, H., Nakamura, M., & Nagahama, Y. (1997). Isolation and characterization of the cDNA encoding the tilapia (Oreochromis niloticus) cytochrome P450 aromatase (P450arom): changes in P450arom mRNA, protein and enzyme activity in ovarian follicles during oogenesis. Journal of Molecular Endocrinology, 18(1), 57-66. doi:10.1677/jme.0.0180057 es_ES
dc.description.references Choi, J. Y., Park, J. G., Jeong, H. B., Lee, Y. D., Takemura, A., & Kim, S. J. (2005). Molecular cloning of cytochrome P450 aromatases in the protogynous wrasse, Halichoeres tenuispinis. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 141(1), 49-59. doi:10.1016/j.cbpc.2005.01.009 es_ES
dc.description.references Diotel, N., Page, Y. L., Mouriec, K., Tong, S.-K., Pellegrini, E., Vaillant, C., … Kah, O. (2010). Aromatase in the brain of teleost fish: Expression, regulation and putative functions. Frontiers in Neuroendocrinology, 31(2), 172-192. doi:10.1016/j.yfrne.2010.01.003 es_ES
dc.description.references Dufour, S., Lopez, E., Le Menn, F., Le Belle, N., Baloche, S., & Fontaine, Y. A. (1988). Stimulation of gonadotropin release and of ovarian development, by the administration of a gonadoliberin agonist and of dopamine antagonists, in female silver eel pretreated with estradiol. General and Comparative Endocrinology, 70(1), 20-30. doi:10.1016/0016-6480(88)90090-1 es_ES
dc.description.references Dufour, S., Sebert, M.-E., Weltzien, F.-A., Rousseau, K., & Pasqualini, C. (2010). Neuroendocrine control by dopamine of teleost reproduction. Journal of Fish Biology, 76(1), 129-160. doi:10.1111/j.1095-8649.2009.02499.x es_ES
dc.description.references Gelinas, D., A. Pitoc, G., & V. Callard, G. (1998). Isolation of a goldfish brain cytochrome P450 aromatase cDNA: Molecular and Cellular Endocrinology, 138(1-2), 81-93. doi:10.1016/s0303-7207(98)00015-x es_ES
dc.description.references Harada, N. (1988). Cloning of a complete cDNA encoding human aromatase : Immunochemical identification and sequence analysis. Biochemical and Biophysical Research Communications, 156(2), 725-732. doi:10.1016/s0006-291x(88)80903-3 es_ES
dc.description.references Harbott, L. K., Burmeister, S. S., White, R. B., Vagell, M., & Fernald, R. D. (2007). Androgen receptors in a cichlid fish,Astatotilapia burtoni: Structure, localization, and expression levels. The Journal of Comparative Neurology, 504(1), 57-73. doi:10.1002/cne.21435 es_ES
dc.description.references Henkel, C. V., Burgerhout, E., de Wijze, D. L., Dirks, R. P., Minegishi, Y., Jansen, H. J., … van den Thillart, G. E. E. J. M. (2012). Primitive Duplicate Hox Clusters in the European Eel’s Genome. PLoS ONE, 7(2), e32231. doi:10.1371/journal.pone.0032231 es_ES
dc.description.references Hildahl, J., Sandvik, G. K., Edvardsen, R. B., Fagernes, C., Norberg, B., Haug, T. M., & Weltzien, F.-A. (2011). Identification and gene expression analysis of three GnRH genes in female Atlantic cod during puberty provides insight into GnRH variant gene loss in fish. General and Comparative Endocrinology, 172(3), 458-467. doi:10.1016/j.ygcen.2011.04.010 es_ES
dc.description.references Ijiri, S., Kazeto, Y., Mark Lokman, P., Adachi, S., & Yamauchi, K. (2003). Characterization of a cDNA Encoding P-450 aromatase (CYP19) from Japanese eel ovary and its expression in ovarian follicles during induced ovarian development. General and Comparative Endocrinology, 130(2), 193-203. doi:10.1016/s0016-6480(02)00589-0 es_ES
dc.description.references Ikeuchi, T., Todo, T., Kobayashi, T., & Nagahama, Y. (1999). cDNA Cloning of a Novel Androgen Receptor Subtype. Journal of Biological Chemistry, 274(36), 25205-25209. doi:10.1074/jbc.274.36.25205 es_ES
dc.description.references Ikeuchi, T., Todo, T., Kobayashi, T., & Nagahama, Y. (2001). Two subtypes of androgen and progestogen receptors in fish testes. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 129(2-3), 449-455. doi:10.1016/s1096-4959(01)00375-x es_ES
dc.description.references Jeng, S.-R., Dufour, S., & Chang, C.-F. (2005). Differential expression of neural and gonadal aromatase enzymatic activities in relation to gonadal development in Japanese eel,Anguilla japonica. Journal of Experimental Zoology Part A: Comparative Experimental Biology, 303A(9), 802-812. doi:10.1002/jez.a.194 es_ES
dc.description.references Kazeto, Y., Tosaka, R., Matsubara, H., Ijiri, S., & Adachi, S. (2011). Ovarian steroidogenesis and the role of sex steroid hormones on ovarian growth and maturation of the Japanese eel. The Journal of Steroid Biochemistry and Molecular Biology, 127(3-5), 149-154. doi:10.1016/j.jsbmb.2011.03.013 es_ES
dc.description.references Khan, I. A., Lopez, E., & Leloup-Hâtey, J. (1987). Induction of spermatogenesis and spermiation by a single injection of human chorionic gonadotropin in intact and hypophysectomized immature European eel (Anguilla anguilla L.). General and Comparative Endocrinology, 68(1), 91-103. doi:10.1016/0016-6480(87)90064-5 es_ES
dc.description.references Kishida, M., & Callard, G. V. (2001). Distinct Cytochrome P450 Aromatase Isoforms in Zebrafish (Danio rerio) Brain and Ovary Are Differentially Programmed and Estrogen Regulated during Early Development**This research was supported by grants from the National Science Foundation (IBN-96-05053) and the NIH (P42 ES-07381). The nucleotide sequences reported in this paper have been submitted to the GenBank/EMBL Data Bank with accession numbers AF226619 and AF226620. Endocrinology, 142(2), 740-750. doi:10.1210/endo.142.2.7928 es_ES
dc.description.references Kwon, J. Y., McAndrew, B. J., & Penman, D. J. (2001). Cloning of brain aromatase gene and expression of brain and ovarian aromatase genes during sexual differentiation in genetic male and female Nile tilapiaOreochromis niloticus. Molecular Reproduction and Development, 59(4), 359-370. doi:10.1002/mrd.1042 es_ES
dc.description.references Levavi-Sivan, B., Biran, J., & Fireman, E. (2006). Sex Steroids Are Involved in the Regulation of Gonadotropin-Releasing Hormone and Dopamine D2 Receptors in Female Tilapia Pituitary1. Biology of Reproduction, 75(4), 642-650. doi:10.1095/biolreprod.106.051540 es_ES
dc.description.references Lin, C.-J., Wu, G.-C., Lee, M.-F., Lau, E.-L., Dufour, S., & Chang, C.-F. (2010). Regulation of two forms of gonadotropin-releasing hormone receptor gene expression in the protandrous black porgy fish, Acanthopagrus schlegeli. Molecular and Cellular Endocrinology, 323(2), 137-146. doi:10.1016/j.mce.2010.04.003 es_ES
dc.description.references Liu, X., Su, H., Zhu, P., Zhang, Y., Huang, J., & Lin, H. (2009). Molecular cloning, characterization and expression pattern of androgen receptor in Spinibarbus denticulatus. General and Comparative Endocrinology, 160(1), 93-101. doi:10.1016/j.ygcen.2008.10.026 es_ES
dc.description.references Lokman, P. M., George, K. A. N., & Young, G. (2003). Effects of steroid and peptide hormones on in vitro growth of previtellogenic oocytes from eel, Anguilla australis. Fish Physiology and Biochemistry, 28(1-4), 283-285. doi:10.1023/b:fish.0000030556.34592.41 es_ES
dc.description.references Lokman, P. M., George, K. A. N., Divers, S. L., Algie, M., & Young, G. (2007). 11-Ketotestosterone and IGF-I increase the size of previtellogenic oocytes from shortfinned eel, Anguilla australis, in vitro. Reproduction, 133(5), 955-967. doi:10.1530/rep-06-0229 es_ES
dc.description.references MATSUBARA, H., KAZETO, Y., IJIRI, S., HIRAI, T., ADACHI, S., & YAMAUCHI, K. (2003). Changes in mRNA levels of ovarian steroidogenic enzymes during artificial maturation of Japanese eel Anguilla japonica. Fisheries Science, 69(5), 979-988. doi:10.1046/j.1444-2906.2003.00716.x es_ES
dc.description.references Matsubara, M., Lokman, P. M., Senaha, A., Kazeto, Y., Ijiri, S., Kambegawa, A., … Yamauchi, K. (2003). Synthesis and possible function of 11-ketotestosterone during oogenesis in eel (Anguilla spp.). Fish Physiology and Biochemistry, 28(1-4), 353-354. doi:10.1023/b:fish.0000030585.22093.7a es_ES
dc.description.references Mazzeo, I., Peñaranda, D. S., Gallego, V., Hildahl, J., Nourizadeh-Lillabadi, R., Asturiano, J. F., … Weltzien, F.-A. (2012). Variations in the gene expression of zona pellucida proteins, zpb and zpc, in female European eel (Anguilla anguilla) during induced sexual maturation. General and Comparative Endocrinology, 178(2), 338-346. doi:10.1016/j.ygcen.2012.06.003 es_ES
dc.description.references Miura, T., Yamauchi, K., Takahashi, H., & Nagahama, Y. (1991). Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica). Proceedings of the National Academy of Sciences, 88(13), 5774-5778. doi:10.1073/pnas.88.13.5774 es_ES
dc.description.references Miura, T., Miura, C., Ohta, T., Nader, M. R., Todo, T., & Yamauchi, K. (1999). Estradiol-17β Stimulates the Renewal of Spermatogonial Stem Cells in Males. Biochemical and Biophysical Research Communications, 264(1), 230-234. doi:10.1006/bbrc.1999.1494 es_ES
dc.description.references Montero, M., Le Belle, N., King, J. A., Millar, R. P., & Dufour, S. (1995). Differential Regulation of the Two Forms of Gonadotropin-Releasing Hormone (mGnRH and cGnRH-II) by Sex Steroids in the European Female Silver Eel (Anguilla anguilla). Neuroendocrinology, 61(5), 525-535. doi:10.1159/000126876 es_ES
dc.description.references Montero, M., Le Belle, N., Vidal, B., & Dufour, S. (1996). Primary Cultures of Dispersed Pituitary Cells from Estradiol-Pretreated Female Silver Eels (Anguilla anguillaL.): Immunocytochemical Characterization of Gonadotropic Cells and Stimulation of Gonadotropin Release. General and Comparative Endocrinology, 104(1), 103-115. doi:10.1006/gcen.1996.0146 es_ES
dc.description.references Rout, U. K., Saed, G. M., & Diamond, M. P. (2005). Reproductive Biology and Endocrinology, 3(1), 1. doi:10.1186/1477-7827-3-1 es_ES
dc.description.references Pasqualini, C., Weltzien, F.-A., Vidal, B., Baloche, S., Rouget, C., Gilles, N., … Dufour, S. (2009). Two Distinct Dopamine D2 Receptor Genes in the European Eel: Molecular Characterization, Tissue-Specific Transcription, and Regulation by Sex Steroids. Endocrinology, 150(3), 1377-1392. doi:10.1210/en.2008-0578 es_ES
dc.description.references Peñaranda, D. S., Pérez, L., Gallego, V., Jover, M., Tveiten, H., Baloche, S., … Asturiano, J. F. (2010). Molecular and physiological study of the artificial maturation process in European eel males: From brain to testis. General and Comparative Endocrinology, 166(1), 160-171. doi:10.1016/j.ygcen.2009.08.006 es_ES
dc.description.references Peñaranda, D. S., Mazzeo, I., Hildahl, J., Gallego, V., Nourizadeh-Lillabadi, R., Pérez, L., … Weltzien, F.-A. (2013). Molecular characterization of three GnRH receptor paralogs in the European eel, Anguilla anguilla: Tissue-distribution and changes in transcript abundance during artificially induced sexual development. Molecular and Cellular Endocrinology, 369(1-2), 1-14. doi:10.1016/j.mce.2013.01.025 es_ES
dc.description.references Perez, L., Aturiano, J. F., Tomas, A., Zegrari, S., Barrera, R., Espinos, F. J., … Jover, M. (2000). Induction of maturation and spermiation in the male European eel: assessment of sperm quality throughout treatment. Journal of Fish Biology, 57(6), 1488-1504. doi:10.1111/j.1095-8649.2000.tb02227.x es_ES
dc.description.references Pérez, L., Peñaranda, D. S., Dufour, S., Baloche, S., Palstra, A. P., Van Den Thillart, G. E. E. J. M., & Asturiano, J. F. (2011). Influence of temperature regime on endocrine parameters and vitellogenesis during experimental maturation of European eel (Anguilla anguilla) females. General and Comparative Endocrinology, 174(1), 51-59. doi:10.1016/j.ygcen.2011.08.009 es_ES
dc.description.references Pfaffl, M. W., Tichopad, A., Prgomet, C., & Neuvians, T. P. (2004). Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations. Biotechnology Letters, 26(6), 509-515. doi:10.1023/b:bile.0000019559.84305.47 es_ES
dc.description.references Sperry, T. S., & Thomas, P. (1999). Characterization of Two Nuclear Androgen Receptors in Atlantic Croaker: Comparison of Their Biochemical Properties and Binding Specificities*. Endocrinology, 140(4), 1602-1611. doi:10.1210/endo.140.4.6631 es_ES
dc.description.references Sperry, T. S., & Thomas, P. (1999). Identification of Two Nuclear Androgen Receptors in Kelp Bass (Paralabrax clathratus) and Their Binding Affinities for Xenobiotics: Comparison with Atlantic Croaker (Micropogonias undulatus) Androgen Receptors1. Biology of Reproduction, 61(4), 1152-1161. doi:10.1095/biolreprod61.4.1152 es_ES
dc.description.references Todo, T., Ikeuchi, T., Kobayashi, T., & Nagahama, Y. (1999). Fish Androgen Receptor: cDNA Cloning, Steroid Activation of Transcription in Transfected Mammalian Cells, and Tissue mRNA Levels. Biochemical and Biophysical Research Communications, 254(2), 378-383. doi:10.1006/bbrc.1998.9919 es_ES
dc.description.references Tosaka, R., Todo, T., Kazeto, Y., Mark Lokman, P., Ijiri, S., Adachi, S., & Yamauchi, K. (2010). Expression of androgen receptor mRNA in the ovary of Japanese eel, Anguilla japonica, during artificially induced ovarian development. General and Comparative Endocrinology, 168(3), 424-430. doi:10.1016/j.ygcen.2010.05.005 es_ES
dc.description.references Trudeau, V. L., Peter, R. E., & Sloley, B. D. (1991). Testosterone and Estradiol Potentiate the Serum Gonadotropin Response to Gonadotropin-Releasing Hormone in Goldfish1. Biology of Reproduction, 44(6), 951-960. doi:10.1095/biolreprod44.6.951 es_ES
dc.description.references Tzchori, I., Degani, G., Hurvitz, A., & Moav, B. (2004). Cloning and developmental expression of the cytochrome P450 aromatase gene (CYP19) in the European eel (Anguilla anguilla). General and Comparative Endocrinology, 138(3), 271-280. doi:10.1016/j.ygcen.2004.06.007 es_ES
dc.description.references Vidal, B., Pasqualini, C., Le Belle, N., Holland, M. C. H., Sbaihi, M., Vernier, P., … Dufour, S. (2004). Dopamine Inhibits Luteinizing Hormone Synthesis and Release in the Juvenile European Eel: A Neuroendocrine Lock for the Onset of Puberty1. Biology of Reproduction, 71(5), 1491-1500. doi:10.1095/biolreprod.104.030627 es_ES
dc.description.references Weltzien, F.-A., Pasqualini, C., Vernier, P., & Dufour, S. (2005). A quantitative real-time RT-PCR assay for European eel tyrosine hydroxylase. General and Comparative Endocrinology, 142(1-2), 134-142. doi:10.1016/j.ygcen.2004.12.019 es_ES
dc.description.references Weltzien, F.-A., Pasqualini, C., Sébert, M.-E., Vidal, B., Le Belle, N., Kah, O., … Dufour, S. (2006). Androgen-Dependent Stimulation of Brain Dopaminergic Systems in the Female European Eel (Anguilla anguilla). Endocrinology, 147(6), 2964-2973. doi:10.1210/en.2005-1477 es_ES


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