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Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla

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Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla

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dc.contributor.author Sorensen, SR es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Butts, IAE es_ES
dc.contributor.author Tomkiewicz, J. es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.date.accessioned 2016-05-26T11:18:35Z
dc.date.available 2016-05-26T11:18:35Z
dc.date.issued 2013-12
dc.identifier.issn 0936-6768
dc.identifier.uri http://hdl.handle.net/10251/64788
dc.description.abstract [EN] Contents European eel, Anguilla anguilla, is a target species for future captive breeding, yet best methodology to estimate sperm density for application in in vitro fertilization is not established. Thus, our objectives were to evaluate methods to estimate European eel sperm density including spermatocrit, computer-assisted sperm analysis (CASA) and flow cytometry (FCM), using Neubauer Improved haemocytometer as benchmark. Initially, relationships between spermatocrit, haemocytometer counts and sperm motility were analysed, as well as the effect of sperm dilution on haemocytometer counts. Furthermore, accuracy and precision of spermatocrit, applying a range of G-forces, were tested and the best G-force used in method comparisons. We found no effect of dilution on haemocytometer sperm density estimates, whereas motility associated positively with haemocytometer counts, but not with spermatocrit. Results from all techniques, spermatocrit, CASA and FCM, showed significant positive correlations with haemocytometer counts. The best correlation between spermatocrit and haemocytometer counts was obtained at 6000xg (r=0.68). Of two CASA variants, one or three photographic fields (CASA-1 and CASA-2), CASA-2 showed a very high accuracy to haemocytometer counts (r=0.93), but low precision (CV: CASA-2=28.4%). FCM was tested with and without microfluorospheres (FCM-1 and FCM-2), and relationships to haemocytometer counts were highly accurate (FCM-1: r=0.94; FCM-2: r=0.88) and precise (CV: FCM-1=2.5; FCM-2=2.7%). Overall, CASA-2 and FCM-1 feature reliable methods for quantification of European eel sperm, but FCM-1 has a clear advantage featuring highest precision and accuracy. Together, these results provide a useful basis for gamete management in fertilization protocols. es_ES
dc.description.sponsorship This work was conducted as part of the project 'Reproduction of European Eel: towards a Self-sustained Aquaculture' (PRO-EEL) supported financially by the European Commission's 7th Framework Programme under the Theme 2 'Food, Agriculture and Fisheries, and Biotechnology', Grant Agreement no245257. Juan F. Asturiano and Luz Perez received a grant to stay in Denmark from Programa de Apoyo a la Investigacion y Desarrollo (PAID-00-11) of the Universitat Politecnica de Valencia. Victor Gallego has a predoctoral grant from Spanish Ministry of Science and Innovation (MICINN). Fish were raised at a commercial eel farm in Jutland, Denmark (Stensgard Eel Farm A/S). We want to thank Peter Lauesen, Billund Aquaculture Service, Christian Graver, Danish Eel Producers Association and Maria K. Johnsen, Technical University of Denmark, for help and assistance in experiments. All fish were handled in accordance with the European Union regulations concerning the protection of experimental animals (Dir 86/609/EEC). en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Reproduction in Domestic Animals es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Turbot scophthalmus-maximus es_ES
dc.subject COD Gadus-morhua es_ES
dc.subject Semen analysis es_ES
dc.subject Hippoglossus-hippoglossus es_ES
dc.subject Fertilization success es_ES
dc.subject Spermatozoa density es_ES
dc.subject Testis development es_ES
dc.subject Gamete contact es_ES
dc.subject Quality evaluation es_ES
dc.subject Seminal plasma es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/rda.12189
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-00-11/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/245257/EU/Reproduction of European Eel: Towards a Self-sustained Aquaculture/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Sorensen, S.; Gallego Albiach, V.; Pérez Igualada, LM.; Butts, I.; Tomkiewicz, J.; Asturiano Nemesio, JF. (2013). Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla. Reproduction in Domestic Animals. 48(6):936-944. https://doi.org/10.1111/rda.12189 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/rda.12189 es_ES
dc.description.upvformatpinicio 936 es_ES
dc.description.upvformatpfin 944 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 48 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 253795 es_ES
dc.identifier.eissn 1439-0531
dc.contributor.funder European Commission
dc.contributor.funder Universitat Politècnica de València
dc.contributor.funder Ministerio de Ciencia e Innovación
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