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Coordinated ultrastructural and phylogenomic analyses shed light on the hidden phycobiont diversity of Trebouxia microalgae in Ramalina fraxinea.

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Coordinated ultrastructural and phylogenomic analyses shed light on the hidden phycobiont diversity of Trebouxia microalgae in Ramalina fraxinea.

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dc.contributor.author Català, Santiago es_ES
dc.contributor.author Campo, Eva del es_ES
dc.contributor.author Barreno, Eva es_ES
dc.contributor.author García-Breijo, Francisco-José es_ES
dc.contributor.author Reig Armiñana, José es_ES
dc.contributor.author Casano, Leonardo es_ES
dc.date.accessioned 2016-05-26T13:09:11Z
dc.date.available 2016-05-26T13:09:11Z
dc.date.issued 2016-01
dc.identifier.issn 1055-7903
dc.identifier.uri http://hdl.handle.net/10251/64812
dc.description.abstract [EN] The precise boundary delineations between taxa in symbiotic associations are very important for evolutionary and ecophysiological studies. Growing evidence indicates that in many cases, the use of either morphological characters or molecular markers results in diversity underestimation. In lichen symbioses. Trebouxia is the most common genus of lichen phycobionts, however, the diversity within this genus has been poorly studied and as such there is no clear species concept. This study constitutes a multifaceted approach incorporating aspects of ultrastructural characterization by TEM and phylogenomics to evaluate the morphological and genetic diversity of phycobionts within the sexually reproducing lichen Ramalina fraxinea in the context of Mediterranean and temperate populations. Results reveal an association with at least seven different Trebouxia lineages belonging to at least two species. T. decolorans and T. jamesii, and diverse combinations of such lineages coexisting within the same thallus depending on the analysed sample. Some of these lineages are shared by several other non-related lichen taxa. Our findings indicate the existence of a highly diverse assemblage of Trebouxia algae associating with R. fraxinea and suggest a possible incipient speciation within T. decolorans rendering a number of lineages or even actual species. This study stresses the importance of coordinated ultrastructural and molecular analyses to improve estimates of diversity and reveal the coexistence of more than one Trebouxia species within the same thallus. lt is also necessary to have clearer species delimitation criteria within the genus Trebouxia and microalgae in general. es_ES
dc.description.sponsorship This study was funded by the Ministerio de Economia y Competitividad (MINECO CGL2012-40058-0O2-01/02), FEDER, the Generalitat Valenciana (PROMETEOII2013/021, GVA) and the Direccion General de Universidades e Investigacion de la Consejeria de Educacion de la Comunidad de Madrid - Universidad de Alcala (CCG10-UAH/GEN-5904). Drs. Arantxa Matins and Patricia Moya (Universitat de Valencia) made helpful comments on the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Ministerio de Economia y Competitividad (MINECO) CGL2012-40058-0O2-01/02 es_ES
dc.relation FEDER es_ES
dc.relation Generalitat Valenciana PROMETEOII2013/021 es_ES
dc.relation Direccion General de Universidades e Investigacion de la Consejeria de Educacion de la Comunidad de Madrid - Universidad de Alcala CCG10-UAH/GEN-5904 es_ES
dc.relation.ispartof Molecular Phylogenetics and Evolution es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Coexistence, cryptic species es_ES
dc.subject Lichen es_ES
dc.subject Ramalina es_ES
dc.subject Symbiosis es_ES
dc.subject Trebouxia es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.subject.classification BIOLOGIA VEGETAL es_ES
dc.title Coordinated ultrastructural and phylogenomic analyses shed light on the hidden phycobiont diversity of Trebouxia microalgae in Ramalina fraxinea. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.ympev.2015.10.021
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.description.bibliographicCitation Català, S.; Campo, ED.; Barreno, E.; García-Breijo, F.; Reig Armiñana, J.; Casano, L. (2016). Coordinated ultrastructural and phylogenomic analyses shed light on the hidden phycobiont diversity of Trebouxia microalgae in Ramalina fraxinea. Molecular Phylogenetics and Evolution. 94:765-777. doi:10.1016/j.ympev.2015.10.021 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.ympev.2015.10.021 es_ES
dc.description.upvformatpinicio 765 es_ES
dc.description.upvformatpfin 777 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 94 es_ES
dc.relation.senia 296462 es_ES
dc.identifier.eissn 1095-9513


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