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The prognostic value of hTERT expression levels in advanced-stage colorectal cancer patients: a comparison between tissue and serum expression

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The prognostic value of hTERT expression levels in advanced-stage colorectal cancer patients: a comparison between tissue and serum expression

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dc.contributor.author Safont, María José es_ES
dc.contributor.author Gil, Mireia es_ES
dc.contributor.author Sirera Pérez, Rafael es_ES
dc.contributor.author Jantus Lewintre, Eloisa es_ES
dc.contributor.author Sanmartín, Elena es_ES
dc.contributor.author Gallach, Sandra es_ES
dc.contributor.author Caballero, Cristina es_ES
dc.contributor.author Pozo, Nieves del es_ES
dc.contributor.author Palomares, Eugenio es_ES
dc.contributor.author Camps, Carlos es_ES
dc.date.accessioned 2016-06-01T11:43:48Z
dc.date.available 2016-06-01T11:43:48Z
dc.date.issued 2011-06
dc.identifier.issn 1699-048X
dc.identifier.uri http://hdl.handle.net/10251/65055
dc.description.abstract [EN] Aim: Telomeres are regions of highly repetitive, non-coding DNA located at the termini of chromosomes whose principal function is to maintain the structural stability of these ends. In 90% of human tumours, telomere length is maintained by the expression and activation of telomerase reverse transcriptase. Various studies have demonstrated an increase in telomerase activity in tumour tissue, which suggests its possible prognostic value. The main objective of our study was to study the prognostic value of the expression level of telomerase catalytic component (hTERT) in patients with colorectal cancer (CRC). Methods: We analysed the prognostic value of the ratio of telomerase expression in tumour tissue to telomerase expression in the adjacent healthy mucosa and the prognostic value of the expression level of hTERT in the serum of patients diagnosed with CRC. As secondary objectives of the study, we (1) analysed the correlation between telomerase expression in the serum and that in the tumour tissue and (2) analysed the relationship between telomerase expression and different clinical parameters. Results: Peripheral blood and tissue samples taken from 48 patients with CRC were analysed. No significant differences were observed in disease-free survival (DFS) or overall survival time (OST) between the groups of patients categorised based on the ratio of telomerase expression between tumour tissue and healthy tissue. The correlation index (Pearson's coefficient) between telomerase levels in the serum and those in tissue was 0.32. Our study of the relationship between telomerase levels in the serum and different clinical variables, such as tumour size, ganglion affectation, preoperative carcinoembryonic antigen levels and stage, revealed a higher telomerase expression level in patients with stage IV CRC. There was no significant association between telomerase expression in tumour tissue and the clinical parameters analysed. Conclusions: The results obtained in our study do not allow us to propose that the level of telomerase expression be used as a prognostic factor in colorectal cancer. Thus, we cannot consider telomerase expression in the serum as a surrogate marker of its expression in tumour tissue. © 2011 Feseo. es_ES
dc.description.sponsorship This work was made possible by a grant from the Spanish Society of Medical Oncology (SEOM). en_EN
dc.language Inglés es_ES
dc.publisher Springer es_ES
dc.relation.ispartof Clinical & Translational Oncology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Colorectal cancer es_ES
dc.subject Marker es_ES
dc.subject Prognostic factor es_ES
dc.subject Serum es_ES
dc.subject Telomerase es_ES
dc.subject Carcinoembryonic antigen es_ES
dc.subject Telomerase reverse transcriptase es_ES
dc.subject Adult es_ES
dc.subject Advanced cancer es_ES
dc.subject Aged es_ES
dc.subject Article es_ES
dc.subject Blood analysis es_ES
dc.subject Cancer patient es_ES
dc.subject Cancer staging es_ES
dc.subject Cancer survival es_ES
dc.subject Controlled study es_ES
dc.subject Disease free survival es_ES
dc.subject Enzyme blood level es_ES
dc.subject Female es_ES
dc.subject Gene expression regulation es_ES
dc.subject Gene overexpression es_ES
dc.subject Human es_ES
dc.subject Human tissue es_ES
dc.subject Male es_ES
dc.subject Marker gene es_ES
dc.subject Overall survival es_ES
dc.subject Prognosis es_ES
dc.subject Protein expression es_ES
dc.subject Survival time es_ES
dc.subject Telomerase gene es_ES
dc.subject Tissue distribution es_ES
dc.subject Tissue level es_ES
dc.subject Tumor volume es_ES
dc.subject Aged, 80 and over es_ES
dc.subject Colon es_ES
dc.subject Colorectal Neoplasms es_ES
dc.subject Follow-Up Studies es_ES
dc.subject Humans es_ES
dc.subject Middle Aged es_ES
dc.subject Reverse Transcriptase Polymerase Chain Reaction es_ES
dc.subject RNA, Messenger es_ES
dc.subject Survival Rate es_ES
dc.subject Tumor Markers, Biological es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title The prognostic value of hTERT expression levels in advanced-stage colorectal cancer patients: a comparison between tissue and serum expression es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s12094-011-0673-2
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Safont, MJ.; Gil, M.; Sirera Pérez, R.; Jantus Lewintre, E.; Sanmartín, E.; Gallach, S.; Caballero, C.... (2011). The prognostic value of hTERT expression levels in advanced-stage colorectal cancer patients: a comparison between tissue and serum expression. Clinical & Translational Oncology. 13(6):396-400. doi:10.1007/s12094-011-0673-2 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1007/s12094-011-0673-2 es_ES
dc.description.upvformatpinicio 396 es_ES
dc.description.upvformatpfin 400 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 220994 es_ES
dc.contributor.funder Sociedad Española de Oncología Médica es_ES
dc.description.references Blasco MA (2005) Telomeres and human disease: ageing, cancer and beyond. Nat Rev Genet 6:611–622 es_ES
dc.description.references Hahn WC, Weinberg RA (2002) Rules for making humor tumor cells. N Engl J Med 347:1593–1603 es_ES
dc.description.references Kim NW, Piatyszek MA, Prowse KR et al (1994) Specific association of human telomerase activity with immortal cells and cancer. Science 266: 2011–2015 es_ES
dc.description.references Hiyama E, Gollahan L, Kataoka T et al (1995) Telomerase activity in gastric cancer. Cancer Res 55:3258–3262 es_ES
dc.description.references Tahara H, Kuniyasu H, Yokozaki H et al (1995) Telomerase activity in preneoplasic and neoplastic gastric and colorectal lesions. Clin Cancer Res 1:1245–1251 es_ES
dc.description.references Hiyama E, Kodama T, Sinbara K et al (1997) Telomerase activity is detected in pancreatic cancer but not in benign tumors. Cancer Res 57: 326–331 es_ES
dc.description.references Engelhardt M, Drullinsky P, Guillem J et al (1997) Telomerase and telomere length in the development and progression of premalignant lesions to colorectal cancer. Clin Cancer Res 3:1931–1941 es_ES
dc.description.references Shay JW, Werbin H, Wright WE (1997) Telomerase assay in the diagnosis and prognosis of cancer. CIBA Found Symp 211:148–155 es_ES
dc.description.references Saleh S, Lam AK, Ho YH (2008) Real-time PCR quantification of human telomerase reverse transcriptase (hTERT) in colorectal cancer. Pathology 40:25–30 es_ES
dc.description.references Kanamaru T, Tanaka K, Kotani J et al (2002) Telomerase activity and hTERT mRNA in development and progression of adenoma to colorectal cancer. Int J Mol Med 10:205–210 es_ES
dc.description.references Gonzalo V, Petit A, Castellví-Bel S et al (2010) Telomerase mRNA expression and immunohis-tochemical detection as a biomarker of malignant transformation in patients with inflammatory bowel disease. Gastroenterol Hepatol 33:288–296 es_ES
dc.description.references Hiyama K, Hirai Y, Kyoizumi et al (1995) Activation of telomerase in human lymphocytes and hematopoietic progenitor cells. J Immunol 155: 3711–3715 es_ES
dc.description.references Taylor RS, Ramirez RD, Ogoshi M et al (1996) Detection of telomerase activity in malignant and nonmalignant skin condition. J Invest Dermatol 106:756–765 es_ES
dc.description.references Hiyama E, Hiyama K, Tatsumoto N et al (1996) Telomerase activity in human intestine. Int J Oncol 9:453–458 es_ES
dc.description.references Tatsumoto N, Hiyama E, Murakami Y et al (2000) High telomerase activity is an independent prognostic indicator of poor outcome in colorectal cancer. Clin Cancer Res 6:2696–2701 es_ES
dc.description.references Gertler R, Rosenberg R, Stricker D et al (2002) Prognostic potential of the telomerase subunit human telomerase reverse transcriptase in tumor tissue and nontumorous mucosa from patients with colorectal carcinoma. Cancer 95:2103–2111 es_ES
dc.description.references Gertler R, Rosenberg R, Stricker D et al (2004) Telomere length and human telomerase reverse transcriptase expression as markers for progression and prognosis on colorectal carcinoma. J Clin Oncol 22:1807–1814 es_ES
dc.description.references Boldrini L, Faviana P, Gisfredi S et al (2004) Regulation of telomerase and its hTERT messenger in colorectal cancer. Oncol Rep 11:395–400 es_ES
dc.description.references Garcia-Aranda C, de Juan C, Diaz-Lopez A et al (2006) Correlations of telomere length, telomerase activity, and telomeric-repeat binding factor 1 expression in colorectal carcinoma. Cancer 106: 541–551 es_ES
dc.description.references Hess JL, Highsmith E (2002) Telomerase detection in body fluids. Clin Chem 48:18–24 es_ES
dc.description.references Cheng X, Bonnefoi H, Pelte M-F et al (2000) Telomerase RNA as a detection marker in the serum of breast cancer patients. Clin Cancer Res 6:3823–3826 es_ES
dc.description.references Ohnishi T, Watanabe T, Nozawa H et al (2008) Telomerase activity of blood samples and recurrence of colorectal cancer. Hepatogastroenterology 55:1513–1518 es_ES


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