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Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan®MGB probes

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Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan®MGB probes

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Debreczeni, DE.; Ruiz Ruiz, S.; Aramburu, J.; López Del Rincón, C.; Belliure Ferrer, BR.; Galipienso Torregrosa, L.; Soler Aleixandre, S.... (2011). Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan®MGB probes. Journal of Virological Methods. 176(1-2):32-37. doi:10.1016/j.jviromet.2011.05.027

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Title: Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan®MGB probes
Author:
UPV Unit: Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Issued date:
Abstract:
A quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) procedure using a general primer set and three TaqMan (R) MGB probes was developed for general and genotype-specific detection and quantitation ...[+]
Subjects: TSWV , Tospovirus , Diagnosis , Quantitative RT-PCR
Copyrigths: Cerrado
Source:
Journal of Virological Methods. (issn: 0166-0934 )
DOI: 10.1016/j.jviromet.2011.05.027
Publisher:
Elsevier
Publisher version: http://dx.doi.org/10.1016/j.jviromet.2011.05.027
Thanks:
This work was supported by grants INIA RTA2008-00010-C03 and Generalitat Valenciana ACOMP/2009/103 and ACOMP/2010/085. D.E. Debreczeni is the recipient of a FPU pre-doctoral fellowship from MEC and B. Belliure was supported ...[+]
Type: Artículo

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