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Effect of in vitro and in vivo conditions on development of parthenogenetic rabbit embryos after vitrification

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Effect of in vitro and in vivo conditions on development of parthenogenetic rabbit embryos after vitrification

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dc.contributor.author Naturil Alfonso, Carmen es_ES
dc.contributor.author Jiménez Trigos, María Estrella es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.date.accessioned 2016-06-03T07:39:23Z
dc.date.available 2016-06-03T07:39:23Z
dc.date.issued 2015-08
dc.identifier.issn 0011-2240
dc.identifier.uri http://hdl.handle.net/10251/65159
dc.description.abstract [EN] Parthenote embryos offer multiple opportunities in biotechnological research, so it is important to analyse the possibilities for their cryopreservation in order to establish a biobank. The aim of this experiment was to determine the effect of culture conditions and vitrification on rabbit parthenogenetic embryos. Parthenotes were cultured under in vivo and in vitro conditions until day 3 (late morula/early blastocyst), when they were vitrified. Immediately after warming, they were newly cultured under in vivo and in vitro conditions till day 6 (blastocyst stage). Both culture conditions showed similar late morula/early blastocyst (0.39±0.056 vs. 0.46±0.043, for in vivo and in vitro, respectively) and blastocyst rates (0.12±0.068 vs. 0.13±0.070, for in vivo and in vitro, respectively). However, no parthenote was recovered when a combination of culture conditions was performed. To our best knowledge, this is the first demonstration of the ability of rabbit parthenogenetic embryos to develop after vitrification, with similar embryo development after in vivo or in vitro culture. Nevertheless, our results highlight the importance of culture conditions on the morphology of parthenote embryos. Therefore, we have described that special attention should be paid on culture conditions to generate parthenote embryos, with a view to their subsequent use, for example in embryonic stem cell production. es_ES
dc.description.sponsorship Statement of funding: This work was supported by funds from the Generalitat Valenciana Research Programme (Prometeoll 2014/036). en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Generalitat Valenciana Research Programme 2014/036 es_ES
dc.relation.ispartof Cryobiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject In vitro es_ES
dc.subject In vivo es_ES
dc.subject Parthenogenesis es_ES
dc.subject Rabbit es_ES
dc.subject Vitrification es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Effect of in vitro and in vivo conditions on development of parthenogenetic rabbit embryos after vitrification es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.cryobiol.2015.05.003
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Naturil Alfonso, C.; Jiménez Trigos, ME.; Vicente Antón, JS.; Marco Jiménez, F. (2015). Effect of in vitro and in vivo conditions on development of parthenogenetic rabbit embryos after vitrification. Cryobiology. 71(1):91-96. doi:10.1016/j.cryobiol.2015.05.003 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.cryobiol.2015.05.003 es_ES
dc.description.upvformatpinicio 91 es_ES
dc.description.upvformatpfin 96 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 71 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 292337 es_ES
dc.identifier.eissn 1090-2392


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