Marco Jiménez, F.; Jiménez Trigos, ME.; Lavara García, R.; Vicente Antón, JS. (2014). Use of cyclodextrins to increase cytoplasmic cholesterol in rabbit embryos and their impact on live kits derived from vitrified embryos. Cryo Letters. 35(4):320-326. http://hdl.handle.net/10251/65519
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/65519
Title:
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Use of cyclodextrins to increase cytoplasmic cholesterol in rabbit embryos and their impact on live kits derived from vitrified embryos
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Author:
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Marco Jiménez, Francisco
Jiménez Trigos, María Estrella
Lavara García, Raquel
Vicente Antón, José Salvador
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UPV Unit:
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Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
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Issued date:
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Abstract:
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[EN] BACKGROUND: Low cryotolerance in oocytes and embryos is frequently associated with
lipid accumulation in the cytoplasm. OBJECTIVE: This study aimed to evaluate the effect of
cyclodextrin used as a cholesterol ...[+]
[EN] BACKGROUND: Low cryotolerance in oocytes and embryos is frequently associated with
lipid accumulation in the cytoplasm. OBJECTIVE: This study aimed to evaluate the effect of
cyclodextrin used as a cholesterol loader to change cytoplasmic cholesterol content of embryos and
raise their tolerance to cryopreservation. METHODS: In the first experiment compact morulae-early
blastocysts were exposed to CLC (0.11 mM and 0.23 mM cholesterol) for 1 hour. In the second
experiment, embryos were exposed to CLC (0.11 mM and 0.23 mM cholesterol) and then vitrified.
RESULT: Using both concentrations, cytoplasmic cholesterol content was increased. Vitrified groups
demonstrated a lower capacity for embryonic development (in vitro and in vivo) compared to the
control groups. Nevertheless, live young were obtained in all groups. CONCLUSIONS: In
conclusion, we have demonstrated the feasibility of using cyclodextrin as a carrier for cholesterol into
rabbit embryo cytoplasm, although further studies are required to clarify the usefulness of CLC use in
embryo cryopreservation
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Subjects:
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Rabbit
,
Cholesterol
,
Cyclodextrin
,
Mucin coat
,
Offspring
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Copyrigths:
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Cerrado |
Source:
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Cryo Letters. (issn:
0143-2044
) (eissn:
1742-0644
)
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Publisher:
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Cryo Letters
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Publisher version:
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http://www.ingentaconnect.com/content/cryo/cryo
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Project ID:
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info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO09%2F2009%2F125/ES/Efecto de la crioconservación de embriones sobre el desarrollo y el re-establecimiento de poblaciones/
info:eu-repo/grantAgreement/GVA//ACIF%2F2010%2F262/
CICYT/AGL2011-30170-C02-02
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Thanks:
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This work was supported by the Spanish Research Project AGL2011-30170-C02-01 and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella Jiménez was supported by a research grant from the ...[+]
This work was supported by the Spanish Research Project AGL2011-30170-C02-01 and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella Jiménez was supported by a research grant from the Education Ministry of the Valencian Regional
Government (programme VALi+d. ACIF/2010/262). English text version revised by N. Macowan English Language Service.
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Type:
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Artículo
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