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In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds

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In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds

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dc.contributor.author Rogina, Anamarija es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Gallego Ferrer, Gloria es_ES
dc.contributor.author Ivankovic, Marica es_ES
dc.contributor.author Ivankovic, Hrvoje es_ES
dc.date.accessioned 2016-06-10T07:35:00Z
dc.date.available 2016-06-10T07:35:00Z
dc.date.issued 2016-04
dc.identifier.issn 0090-6964
dc.identifier.uri http://hdl.handle.net/10251/65622
dc.description.abstract Highly porous chitosan/hydroxyapatite composite structures with different weight ratios (100/0; 90/10; 80/20; 70/30; 60/40; 50/50; 40/60) have been prepared by precipitation method and freeze-gelation technique using calcite, urea phosphate and chitosan as starting materials. The composition of prepared composite scaffolds was characterized by X-ray diffraction analysis and Fourier transformed infrared spectroscopy, while morphology of scaffolds was imaged by scanning electron microscopy. Mercury intrusion porosimetry measurements of prepared scaffolds have shown different porosity and microstructure regarding to the HA content, along with SEM observations of scaffolds after being immersed in physiological medium. The results of swelling capacity and compressive strength measured in Dulbecco’s phosphate buffer saline (DPBS) have shown higher values for composite scaffolds with lower in situ HA content. Viability, proliferation and differentiation of MC3T3-E1 cells seeded on different scaffolds have been evaluated by live dead assay and confocal scan microscopy. Our results suggest that the increase of HA content enhance osteoblast differentiation confirming osteogenic properties of highly porous CS/HA scaffolds for tissue engineering applications in bone repair. es_ES
dc.description.sponsorship The financial support of the Croatian Science Foundation (project: "Development of Biocompatible Hydroxyapatite Based Materials for Bone Tissue Engineering Applications") and L'Oreal-UNESCO Foundation 'For Women in Science' is gratefully acknowledged. The financial support from the Spanish Ministry of Economy and Competitiveness and the Feder funds through the MAT2013-46467-C4-1-R project is acknowledged by the Spanish co-authors. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. The authors want to acknowledge Pilar Gomez Tena and Sergio Mestre Beltran from Instituto de Tecnologia Ceramica, Castellon, Spain, for theirs assistance with porosity measurements. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Annals of Biomedical Engineering es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Chitosan es_ES
dc.subject Hydroxyapatite es_ES
dc.subject Scaffold es_ES
dc.subject Unconfined compression es_ES
dc.subject Porosity es_ES
dc.subject MC3T3-E1 differentiation es_ES
dc.subject Osteogenic markers es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10439-015-1418-0
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2013-46467-C4-1-R/ES/ESTIMULACION MECANICA LOCAL DE CELULAS MESENQUIMALES DE CARA A SU DIFERENCIACION OSTEOGENICA Y CONDROGENICA EN MEDICINA REGENERATIVA/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Rogina, A.; Rico Tortosa, PM.; Gallego Ferrer, G.; Ivankovic, M.; Ivankovic, H. (2016). In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds. Annals of Biomedical Engineering. 44(4):1107-1119. https://doi.org/10.1007/s10439-015-1418-0 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10439-015-1418-0 es_ES
dc.description.upvformatpinicio 1107 es_ES
dc.description.upvformatpfin 1119 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 44 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 306338 es_ES
dc.identifier.eissn 1573-9686
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder L'Oréal es_ES
dc.contributor.funder Croatian Science Foundation es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
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