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Site-specific immobilization of DNA on silicon surfaces by using the thiol-yne reaction

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Site-specific immobilization of DNA on silicon surfaces by using the thiol-yne reaction

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dc.contributor.author Escorihuela Fuentes, Jorge es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2016-06-14T06:57:45Z
dc.date.available 2016-06-14T06:57:45Z
dc.date.issued 2014
dc.identifier.issn 2050-750X
dc.identifier.uri http://hdl.handle.net/10251/65817
dc.description.abstract [EN] Covalent immobilization of ssDNA fragments onto silicon-based materials was performed using the thiol-yne reaction. Chemical functionalization provided alkyne groups on the surface where the thiol-modified oligonucleotide probes can be easily photoattached as microarrays, reaching an immobilization density around 30 pmol cm(-2). The developed method presents the advantages of spatially controlled probe anchoring (by using a photomask), direct attachment without using cross-linkers, and short irradiation times (20 min). Hybridization efficiencies up to 70%, with full complementary strands, were reached. The approach was evaluated by scoring single nucleotide polymorphisms with a discrimination ratio around 15. Moreover, the potential applicability of the proposed methodology is demonstrated through the specific detection of 20 nM of a genomic target of bacterial Escherichia coli. es_ES
dc.description.sponsorship This research was supported by Ministerio de Ciencia e Innovacion (CTQ2013-45875-R) and Generalitat Valenciana (PROMETEO/2010/008). en_EN
dc.language Inglés es_ES
dc.publisher Royal Society of Chemistry es_ES
dc.relation.ispartof Journal of Materials Chemistry B es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject ENE CLICK CHEMISTRY es_ES
dc.subject PLASMON RESONANCE es_ES
dc.subject NUCLEIC-ACIDS es_ES
dc.subject REAL-TIME es_ES
dc.subject MICROARRAYS es_ES
dc.subject XPS es_ES
dc.subject MONOLAYERS es_ES
dc.subject FUTURE es_ES
dc.subject ARRAYS es_ES
dc.subject FUNCTIONALIZATION es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Site-specific immobilization of DNA on silicon surfaces by using the thiol-yne reaction es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1039/c4tb01108b
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F008/ES/AYUDA PARA EL GRUPO SYM-IDM/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Escorihuela Fuentes, J.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, Á. (2014). Site-specific immobilization of DNA on silicon surfaces by using the thiol-yne reaction. Journal of Materials Chemistry B. 2(48):8510-8517. https://doi.org/10.1039/c4tb01108b es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1039/c4tb01108b es_ES
dc.description.upvformatpinicio 8510 es_ES
dc.description.upvformatpfin 8517 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 2 es_ES
dc.description.issue 48 es_ES
dc.relation.senia 278982 es_ES
dc.identifier.eissn 2050-7518
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Generalitat Valenciana es_ES


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