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dc.contributor.author | Andújar Pérez, Isabel | es_ES |
dc.contributor.author | Recio, M. C. | es_ES |
dc.contributor.author | Bacelli, T. | es_ES |
dc.contributor.author | Giner, R. M. | es_ES |
dc.contributor.author | Rios, J. L. | es_ES |
dc.date.accessioned | 2016-06-16T11:08:46Z | |
dc.date.available | 2016-06-16T11:08:46Z | |
dc.date.issued | 2010-05 | |
dc.identifier.issn | 0007-1188 | |
dc.identifier.uri | http://hdl.handle.net/10251/66022 | |
dc.description.abstract | [EN] Background and purpose: In the present paper we studied the effect of shikonin on ear oedema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA), and determined the mechanisms through which shikonin might exert its topical anti-inflammatory action. Experimental approach: Acute ear oedema was induced in mice by topical application of TPA. The in vitro assays used macrophages RAW 264.7 cells stimulated with lipopolysaccharide. Cyclooxygenase-2, inducible nitric oxide synthase, protein kinase C alpha, extracellular signal-regulated protein kinase (ERK), phosphorylated ERK (pERK), c-Jun N-terminal kinase (JNK), pJNK, p38, p-p38, p65, p-p65, inhibitor protein of nuclear factor-kappa B (NF-kappa B) (I kappa B alpha) and pI kappa B alpha were measured by Western blotting, activation and binding of NF-kappa B to DNA was detected by reporter gene and electrophoretic mobility shift assay, respectively, and NF-kappa B p65 localization was detected by immunocytochemistry. Key results: Shikonin reduced the oedema (inhibitory dose 50 = 1.0 mg per ear), the expression of cyclooxygenase-2 (70%) and of inducible nitric oxide synthase (100%) in vivo. It significantly decreased TPA-induced translocation of protein kinase C alpha, the phosphorylation and activation of ERK, the nuclear translocation of NF-kappa B and the TPA-induced NF-kappa B-DNA-binding activity in mouse skin. Moreover, in RAW 264.7 cells, shikonin significantly inhibited the binding of NF-kappa B to DNA in a dose-dependent manner and the nuclear translocation of p65. Conclusions and implications: Shikonin exerted its topical anti-inflammatory action by interfering with the degradation of I kappa B alpha, thus inhibiting the activation of NF-kappa B. | es_ES |
dc.description.sponsorship | This work was supported by the Spanish Government (SAF2006-06726) and Generalitat Valenciana (GVPRE/2008/ 387). I. Andújar is grateful for a fellowship from the Generalitat Valenciana, grant BFPI/2008/040. | |
dc.language | Inglés | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation.ispartof | British Journal of Pharmacology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Shikonin | es_ES |
dc.subject | TPA | es_ES |
dc.subject | Cyclooxygenase-2 | es_ES |
dc.subject | Ear mouse | es_ES |
dc.subject | RAW 264 | es_ES |
dc.subject | 7 macrophages | es_ES |
dc.subject | NF-kappa B | es_ES |
dc.subject | P65 | es_ES |
dc.subject | Inducible nitric oxide synthase | es_ES |
dc.subject | MAPK | es_ES |
dc.title | Shikonin reduces oedema induced by phorbol ester by interfering with I kappa B alpha degradation thus inhibiting translocation of NF-kappa B to the nucleus | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/j.1476-5381.2010.00696.x | |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//SAF2006-06726/ES/MODULACION DE LA ACTIVIDAD STAT3 Y STAT4 COMO POSIBLE MECANISMO DE ACCION DE AGENTES ANTIINFLAMATORIOS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GVPRE%2F2008%2F387/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//BFPI%2F2008%2F040/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.description.bibliographicCitation | Andújar Pérez, I.; Recio, MC.; Bacelli, T.; Giner, RM.; Rios, JL. (2010). Shikonin reduces oedema induced by phorbol ester by interfering with I kappa B alpha degradation thus inhibiting translocation of NF-kappa B to the nucleus. British Journal of Pharmacology. 160(2):376-388. https://doi.org/10.1111/j.1476-5381.2010.00696.x | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1111/j.1476-5381.2010.00696.x | es_ES |
dc.description.upvformatpinicio | 376 | es_ES |
dc.description.upvformatpfin | 388 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 160 | es_ES |
dc.description.issue | 2 | es_ES |
dc.relation.senia | 238312 | es_ES |
dc.identifier.pmid | 20423347 | |
dc.identifier.pmcid | PMC2874859 | |
dc.contributor.funder | Ministerio de Educación y Ciencia | |
dc.contributor.funder | Generalitat Valenciana |