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Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA.

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Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA.

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dc.contributor.author Eiras, Marcelo es_ES
dc.contributor.author Nohales Zafra, Maria Angeles es_ES
dc.contributor.author Kitajima, Elliot J. es_ES
dc.contributor.author Flores Pedauye, Ricardo es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2016-06-17T11:01:53Z
dc.date.available 2016-06-17T11:01:53Z
dc.date.issued 2011-03
dc.identifier.issn 0304-8608
dc.identifier.uri http://hdl.handle.net/10251/66089
dc.description.abstract [EN] Potato spindle tuber viroid (PSTVd) contains an element of tertiary structure -loop E- also present in eukaryotic 5S rRNA. The ribosomal protein L5 and transcription factor IIIA (TFIIIA) from Arabidopsis thaliana bind 5S rRNA in vitro and in vivo, mediating different functions that include nucleocytoplasmic transport and transcription activation, respectively. We show that A. thaliana L5 and TFIIIA also bind PSTVd (+) RNA in vitro with the same affinity as they bind 5S rRNA, whereas the affinity for a chloroplastic viroid is significantly lower. These two proteins might participate in the synthesis and delivery of PSTVd RNA in vivo. es_ES
dc.description.sponsorship This work was supported by grants BIO200801986 and BFU2008-03154/BMC from the Spanish Ministerio de Ciencia e Innovacion (MICINN) and ACOMP/2009/042 and ACOMP/2010/278 from Generalitat Valenciana to J. A. D and R. F., respectively. M. E. and M. A. N were recipients of predoctoral fellowships from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (Brazil) and MICINN, respectively. We thank Dr. S. Tourmente (UniversiteBlaise Pascal, Aubiere, France) for 5S rRNA, TFIIIA and L5 cDNA clones. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Generalitat Valenciana ACOMP/2010/278 es_ES
dc.relation Generalitat Valenciana ACOMP/2009/042 es_ES
dc.relation Spanish Ministerio de Ciencia e Innovacion (MICINN) BFU2008-03154/BMC es_ES
dc.relation Spanish Ministerio de Ciencia e Innovacion (MICINN) BIO200801986 es_ES
dc.relation Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (Brazil) es_ES
dc.relation.ispartof Archives of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject LOOP-E-MOTIF es_ES
dc.subject HOST INTERACTIONS es_ES
dc.subject VIVO es_ES
dc.subject EXISTENCE es_ES
dc.subject CLEAVAGE es_ES
dc.title Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00705-010-0867-x
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Eiras, M.; Nohales Zafra, MA.; Kitajima, EJ.; Flores Pedauye, R.; Daros Arnau, JA. (2011). Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA. Archives of Virology. 156(3):529-533. doi:10.1007/s00705-010-0867-x es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1007/s00705-010-0867-x es_ES
dc.description.upvformatpinicio 529 es_ES
dc.description.upvformatpfin 533 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 156 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 216039 es_ES
dc.identifier.eissn 1432-8798
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