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Covalent Immobilization of Lysozyme on Ethylene Vinyl Alcohol Films for Nonmigrating Antimicrobial Packaging Applications

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Covalent Immobilization of Lysozyme on Ethylene Vinyl Alcohol Films for Nonmigrating Antimicrobial Packaging Applications

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dc.contributor.author Muriel Galet, Virginia es_ES
dc.contributor.author Talbert, J.N. es_ES
dc.contributor.author Hernandez Muñoz, P. es_ES
dc.contributor.author GAVARA CLEMENTE, RAFAEL es_ES
dc.contributor.author Goddard, J.M. es_ES
dc.date.accessioned 2016-06-17T12:11:26Z
dc.date.issued 2013-07-10
dc.identifier.issn 0021-8561
dc.identifier.uri http://hdl.handle.net/10251/66094
dc.description.abstract [EN] The objective of this study was to develop a new antimicrobial film, in which lysozyme was covalently attached onto two different ethylene vinyl alcohol copolymers (EVOH 29 and EVOH 44). The EVOH surface was modified with UV irradiation treatment to generate carboxylic acid groups, and lysozyme was covalently attached to the functionalized polymer surface. Surface characterization of control and modified films was performed using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and dye assay. The value of protein loading after attachment on the surface was 8.49 μg protein/cm2 and 5.74 μg protein/cm2 for EVOH 29 and EVOH 44, respectively, after 10 min UV irradiation and bioconjugation. The efficacy of the EVOH−lysozyme films was assessed using Micrococcus lysodeikticus. The antimicrobial activity of the films was tested against Listeria monocytogenes and was similar to an equivalent amount of free enzyme. The reduction was 1.08 log for EVOH 29−lysozyme, 0.95 log for EVOH 44−lysozyme, and 1.34 log for free lysozyme. This work confirmed the successful use of lysozyme immobilization on the EVOH surface for antimicrobial packaging es_ES
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation.ispartof Journal of Agricultural and Food Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Lysozyme es_ES
dc.subject EVOH es_ES
dc.subject Immobilization es_ES
dc.subject UV irradiation es_ES
dc.subject L. monocytogenes es_ES
dc.subject.classification TECNOLOGIA DE ALIMENTOS es_ES
dc.title Covalent Immobilization of Lysozyme on Ethylene Vinyl Alcohol Films for Nonmigrating Antimicrobial Packaging Applications es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/jf401818u
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Tecnología de Alimentos - Departament de Tecnologia d'Aliments es_ES
dc.description.bibliographicCitation Muriel Galet, V.; Talbert, J.; Hernandez Muñoz, P.; Gavara Clemente, R.; Goddard, J. (2013). Covalent Immobilization of Lysozyme on Ethylene Vinyl Alcohol Films for Nonmigrating Antimicrobial Packaging Applications. Journal of Agricultural and Food Chemistry. 61(27):6720-6727. doi:10.1021/jf401818u es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1021/jf401818u es_ES
dc.description.upvformatpinicio 6720 es_ES
dc.description.upvformatpfin 6727 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 61 es_ES
dc.description.issue 27 es_ES
dc.relation.senia 249600 es_ES


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