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Pectinmethylesterase extraction from orange solid wastes: Optimization and comparison between conventional and ultrasound-assisted treatments

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Pectinmethylesterase extraction from orange solid wastes: Optimization and comparison between conventional and ultrasound-assisted treatments

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dc.contributor.author Rodríguez López, Antonio Diego es_ES
dc.contributor.author Mayor López, Luis es_ES
dc.contributor.author Galfarsoro Machado, María Milagros es_ES
dc.contributor.author Martínez Otalo, Jorge Leonardo es_ES
dc.contributor.author Garcia-Castello, Esperanza M. es_ES
dc.date.accessioned 2016-06-22T15:06:35Z
dc.date.available 2016-06-22T15:06:35Z
dc.date.issued 2013-09
dc.identifier.issn 2156-8553
dc.identifier.uri http://hdl.handle.net/10251/66310
dc.description.abstract [EN] During orange juice production, a half of fresh oranges weight is considered as production waste (peels, pulp, seeds, orange leaves and damaged orange fruits). An alternative for the management of these wastes is their treatment by addition of lime and a latter pressing, obtaining a press cake and a press liquor rich in sugars (10˚ Brix) and citric acid, protein, pectin and ethanol. For non-thermal concentration of press liquor to obtain citruss molasses (65˚ - 70˚ Brix), the removal of pectin is necessary. Traditionally, depectinization of juices has been done by using pectinmethylesterase (PME) enzymes from external sources. In this work it performed the extraction of PME enzymes from orange peels to obtain the optimum extraction conditions. Two different methods of solvent extraction were compared (conventional and ultrasound-assisted methods). For the conventional extraction experiments, a central composite design with three variables ([NaCl], pH and time) and five replicates of the center point was used. For ultrasound-assisted extraction, experiments were done at pH = 5.5 and [NaCl] = 1.25 M), varying extraction time (1 - 30 min). Response variables were PME activity, protein content and a ratio between them, named PME effectiveness (ηPME). At the same experimental conditions (pH =5 .5, [NaCl] = 1.25 M, t = 15 min) it was found that conventional extractions led to slightly better results in terms of ηPME than ultrasound-assisted extraction method. es_ES
dc.description.sponsorship Authors acknowledge the Vicerrectorado de Investigación of the Universidad Politecnica de Valencia for the financial support (project 1965) from the call Proyectos de Nuevas Líneas de Investigación Multidisciplinares (PAID05-11).
dc.language Inglés es_ES
dc.publisher Scientific Research Publishing es_ES
dc.relation UPV/PAID05-11 es_ES
dc.relation.ispartof Agricultural Sciences es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Orange Peel es_ES
dc.subject Solid-Liquid Extraction es_ES
dc.subject Ultrasound-Assisted Extraction es_ES
dc.subject Pectinmethylesterase es_ES
dc.subject Press Liquor es_ES
dc.subject.classification INGENIERIA QUIMICA es_ES
dc.title Pectinmethylesterase extraction from orange solid wastes: Optimization and comparison between conventional and ultrasound-assisted treatments es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4236/as.2013.49B008
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería de Alimentos para el Desarrollo - Institut Universitari d'Enginyeria d'Aliments per al Desenvolupament es_ES
dc.description.bibliographicCitation Rodríguez López, AD.; Mayor López, L.; Galfarsoro Machado, MM.; Martínez Otalo, JL.; Garcia-Castello, EM. (2013). Pectinmethylesterase extraction from orange solid wastes: Optimization and comparison between conventional and ultrasound-assisted treatments. Agricultural Sciences. 4(9B):45-50. doi:10.4236/as.2013.49B008 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.4236/as.2013.49B008 es_ES
dc.description.upvformatpinicio 45 es_ES
dc.description.upvformatpfin 50 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 4 es_ES
dc.description.issue 9B es_ES
dc.relation.senia 256894 es_ES
dc.identifier.eissn 2156-8561
dc.contributor.funder Universitat Politècnica de València


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