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Dynamic regulation of cortical microtubule organization through prefoldin-DELLA interaction

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Dynamic regulation of cortical microtubule organization through prefoldin-DELLA interaction

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dc.contributor.author Locascio, Antonella Anna Maria es_ES
dc.contributor.author Blazquez Rodriguez, Miguel Angel es_ES
dc.contributor.author Alabadí Diego, David es_ES
dc.date.accessioned 2016-06-24T10:04:47Z
dc.date.issued 2013-05-06
dc.identifier.issn 0960-9822
dc.identifier.uri http://hdl.handle.net/10251/66422
dc.description.abstract Plant morphogenesis relies on specific patterns of cell division and expansion. It is well established that cortical microtubules influence the direction of cell expansion [1, 2], but less is known about the molecular mechanisms that regulate microtubule arrangement. Here we show that the phytohormones gibberellins (GAs) regulate microtubule orientation through physical interaction between the nuclear-localized DELLA proteins and the prefoldin complex, a cochaperone required for tubulin folding [3]. In the presence of GA, DELLA proteins are degraded, and the prefoldin complex stays in the cytoplasm and is functional. In the absence of GA, the prefoldin complex is localized to the nucleus, which severely compromises a/b-tubulin heterodimer availability, affecting microtubule organization. The physiological relevance of this molecular mechanism was confirmed by the observation that the daily rhythm of plant growth was accompanied by coordinated oscillation of DELLA accumulation, prefoldin subcellular localization, and cortical microtubule reorientation. es_ES
dc.description.sponsorship We thank C. Somerville and Y. Gu for the PFD6-YFP line, J. Salinas for the pfd5 mutant, N. Mann de la Rosa for the myc-GAI construct, and A. Ferrando for the BiFC vectors. We also thank S. Tarraga for assistance with the gel filtration assays and C. Fankhauser, J. Salinas, and P. Rodriguez for discussions and critical reading of the manuscript. This work was supported by grants from the Spanish Ministry of Science and Innovation (BIO2010-15071 and CSD2007-00057) and the Generalitat Valenciana (ACOMP/2011/288 and PROMETEO/2010/020). A.L. was funded during part of her work by Fondo per gli Investimenti della Ricerca di Base of the Italian Ministry of Education, the University, and Research (MIUR). en_EN
dc.language Inglés es_ES
dc.publisher Elsevier (Cell Press) es_ES
dc.relation info:eu-repo/grantAgreement/MEC//CSD2007-00057/ES/Función y potencial biotecnológico de los factores de transcripción de las plantas./ es_ES
dc.relation Generalitat Valenciana (ACOMP/ 2011/288 and PROMETEO/2010/020) es_ES
dc.relation Fondo per gli Investimenti della Ricerca di Base of the Italian Ministry of Education, the University, and Research (MIUR) es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//BIO2010-15071/ES/SEÑALIZACION POR GIBERELINAS DEPENDIENTE DEL CONTEXTO EN ARABIDOPSIS/ es_ES
dc.relation.ispartof Current Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.title Dynamic regulation of cortical microtubule organization through prefoldin-DELLA interaction es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1016/j.cub.2013.03.053
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Locascio, AAM.; Blazquez Rodriguez, MA.; Alabadí Diego, D. (2013). Dynamic regulation of cortical microtubule organization through prefoldin-DELLA interaction. Current Biology. 23:804-809. doi:10.1016/j.cub.2013.03.053 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.cub.2013.03.053 es_ES
dc.description.upvformatpinicio 804 es_ES
dc.description.upvformatpfin 809 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 23 es_ES
dc.relation.senia 259556 es_ES


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