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dc.contributor.author | Pinto, Filipe | es_ES |
dc.contributor.author | Pacheco, Catarina C. | es_ES |
dc.contributor.author | Oliveira, Paulo | es_ES |
dc.contributor.author | Montagud, Arnau | es_ES |
dc.contributor.author | Landels, Andrew | es_ES |
dc.contributor.author | Couto, Narciso | es_ES |
dc.contributor.author | Wright, Phillip C. | es_ES |
dc.contributor.author | Urchueguía Schölzel, Javier Fermín | es_ES |
dc.contributor.author | Tamagnini, Paula | es_ES |
dc.date.accessioned | 2016-06-28T09:10:04Z | |
dc.date.available | 2016-06-28T09:10:04Z | |
dc.date.issued | 2015-12 | |
dc.identifier.issn | 1340-2838 | |
dc.identifier.uri | http://hdl.handle.net/10251/66628 | |
dc.description.abstract | [EN] The use of microorganisms as cell factories frequently requires extensive molecular manipulation. Therefore, the identification of genomic neutral sites for the stable integration of ectopic DNA is required to ensure a successful outcome. Herewe describe the genome mapping and validation of five neutral sites in the chromosome of Synechocystis sp. PCC 6803, foreseeing the use of this cyanobacterium as a photoautotrophic chassis. To evaluate the neutrality of these loci, insertion/deletion mutants were produced, and to assess their functionality, a synthetic green fluorescent reporter module was introduced. The constructed integrative vectors include a BioBrick-compatible multiple cloning site insulated by transcription terminators, constituting robust cloning interfaces for synthetic biology approaches. Moreover, Synechocystis mutants (chassis) ready to receive purpose-built synthetic modules/circuits are also available. This work presents a systematic approach to map and validate chromosomal neutral sites in cyanobacteria, and that can be extended to other organisms. | es_ES |
dc.description.sponsorship | This work was supported by the European Commission through the Seventh Framework Programme, FP7-ENERGY-2012-1-2STAGE-308518 (CyanoFactory), from EU FP6-NEST-2005-Path-SYN project BioModularH2 (contract no. 043340) and from National Funds through Fundacao para a Ciencia e a Tecnologia (FCT) (grants SFRH/BD/36378/2007 to F.P., SFRH/BPD/64095/2009 to C.C.P., SFRH/BPD/74894/2010 to P.O.). We also acknowledge the Engineering and Physical Sciences Research Council (EPSRC) for funding (EP/E036252/1) and The University of Sheffield for Scholarship funding. Funding to pay the Open Access publication charges for this article was provided by the European Commission through the Seventh Framework Programme, FP7-ENERGY-2012-1-2STAGE-308518 (CyanoFactory). | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Oxford University Press (OUP) | es_ES |
dc.relation.ispartof | DNA Research | es_ES |
dc.rights | Reconocimiento - No comercial (by-nc) | es_ES |
dc.subject | Neutral sites | es_ES |
dc.subject | photoautotrophic chassis | es_ES |
dc.subject | Synechocystis | es_ES |
dc.subject | synthetic biology | es_ES |
dc.subject.classification | FISICA APLICADA | es_ES |
dc.title | Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1093/dnares/dsv024 | |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/308518/EU/Design, construction and demonstration of solar biofuel production using novel (photo)synthetic cell factories/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP6/43340/EU/Engineered modular bacterial hydrogen photo-production of hydrogen/BIOMODULARH2/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UKRI//EP%2FE036252%2F1/GB/ChELSI: Chemical Engineering Life Science Interface/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/RCUK/EPSRC/EP/E036252/1/GB/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/36378/2007/PT/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/FCT/SFRH/SFRH/BPD/64095/2009/PT/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F74894%2F2010/PT/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F64095%2F2009/PT/MODULES AND CIRCUITS FOR H2 PRODUCTION, A SYNTHETIC BIOLOGY APPROACH USING SYNECHOCYSTIS SP. PCC 6803 AS A PHOTOAUTOTROPHIC CHASSIS/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F36378%2F2007/PT/ENGINEERING OF A CYANOBACTERIUM FOR BIOHYDROGEN PRODUCTION: A NEW COMPUTATIONAL ASSISTED DESIGN OF A PHOTOAUTOTROPHIC CHASSIS/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F74894%2F2010/PT/EXPLORING PROTEIN LOCALIZATION AND SECRETION TO DEVELOP CYANOBACTERIA AS ROBUST BIOTECHNOLOGICAL TOOLS/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP6/043340/EU/Engineered modular bacterial hydrogen photo-production of hydrogen/BIOMODULARH2/ | |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Matemática Pura y Aplicada - Institut Universitari de Matemàtica Pura i Aplicada | es_ES |
dc.description.bibliographicCitation | Pinto, F.; Pacheco, CC.; Oliveira, P.; Montagud, A.; Landels, A.; Couto, N.; Wright, PC.... (2015). Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites. DNA Research. 22(6):425-437. https://doi.org/10.1093/dnares/dsv024 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/dnares/dsv024 | es_ES |
dc.description.upvformatpinicio | 425 | es_ES |
dc.description.upvformatpfin | 437 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 22 | es_ES |
dc.description.issue | 6 | es_ES |
dc.relation.senia | info:eu-repo/grantAgreement/EC/FP6/301441/EU/Engineered modular bacterial hydrogen photo-production of hydrogen/BIOMODULARH2 | es_ES |
dc.identifier.eissn | 1756-1663 | |
dc.identifier.pmid | 26490728 | en_EN |
dc.identifier.pmcid | PMC4675711 | en_EN |
dc.contributor.funder | European Commission | |
dc.contributor.funder | UK Research and Innovation | es_ES |
dc.contributor.funder | Fundação para a Ciência e a Tecnologia, Portugal | |
dc.contributor.funder | Engineering and Physical Sciences Research Council, Reino Unido |