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Toward a more efficient isolation of total RNA from loquat (Eriobotrya japonica Lindl.) tissues

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Toward a more efficient isolation of total RNA from loquat (Eriobotrya japonica Lindl.) tissues

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dc.contributor.author Martínez Fuentes, Amparo es_ES
dc.contributor.author Mesejo Conejos, Carlos es_ES
dc.contributor.author Agustí Fonfría, Manuel es_ES
dc.contributor.author Reig Valor, Carmina es_ES
dc.date.accessioned 2016-06-29T06:59:49Z
dc.date.available 2016-06-29T06:59:49Z
dc.date.issued 2015-01-20
dc.identifier.issn 0248-1294
dc.identifier.uri http://hdl.handle.net/10251/66723
dc.description.abstract [EN] Loquat (Eriobotrya japonica Lindl.) tissues contain considerable amounts of polyphenolic compounds and polysaccharides which can copurify with RNA. This can render the RNA unsuitable for DNA synthesis and makes the isolation of high quality RNA from loquat tissues particularly difficult. Materials and methods. Total RNA was extracted from leaves, buds and fruit of loquat by adapting the method described by Song et al. in 2011. This involved improvements both to the extraction protocol and RNA purity. Results and discussion. Our modified method resulted in the isolation of high quality RNA with yields 6-20 times higher than that reported for existing methods. We obtained A(260/280) ratios of 2-2.40 A(260/230) ratios of 2-2.31 and RNA integrity numbers (RIN) of 8 to 8.3. The RNA obtained has reached the requested quality for downstream applications such as quantitative real-time polymerase chain reaction (RT-qPCR) experiments. es_ES
dc.language Inglés es_ES
dc.publisher EDP Sciences es_ES
dc.relation.ispartof Fruits es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Loquat es_ES
dc.subject Spain es_ES
dc.subject Spain,loquat,Eriobotrya japonica,phenolics,RNA extraction es_ES
dc.subject Eriobotrya japonica es_ES
dc.subject Phenolics es_ES
dc.subject RNA extraction es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Toward a more efficient isolation of total RNA from loquat (Eriobotrya japonica Lindl.) tissues es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1051/fruits/2014042
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.description.bibliographicCitation Martinez Fuentes, A.; Mesejo Conejos, C.; Agustí Fonfría, M.; Reig Valor, C. (2015). Toward a more efficient isolation of total RNA from loquat (Eriobotrya japonica Lindl.) tissues. Fruits. 70(1):47-51. doi:10.1051/fruits/2014042 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1051/fruits/2014042 es_ES
dc.description.upvformatpinicio 47 es_ES
dc.description.upvformatpfin 51 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 70 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 302580 es_ES
dc.identifier.eissn 1625-967X


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