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dc.contributor.author | Sayas Montañana, Enric Miquel | es_ES |
dc.contributor.author | García López, Federico José | es_ES |
dc.contributor.author | Serrano Salom, Ramón | es_ES |
dc.date.accessioned | 2016-06-30T07:19:59Z | |
dc.date.available | 2016-06-30T07:19:59Z | |
dc.date.issued | 2015-09 | |
dc.identifier.issn | 0749-503X | |
dc.identifier.uri | http://hdl.handle.net/10251/66779 | |
dc.description.abstract | [EN] We have compared the toxicity, mutagenicity and transport in Saccharomyces cerevisiae of three DNA-intercalating fluorescent dyes widely used to stain DNA in gels. Safety data about ethidium bromide (EtBr) are contradictory, and two compounds of undisclosed structure (Redsafe and Gelred) have been proposed as safe alternatives. Our results indicate that all three compounds inhibit yeast growth, with Gelred being the most inhibitory and also the only one causing cell death. EtBr and Gelred, but not Redsafe, induce massive formation of petite (non-respiratory) mutants, but only EtBr induces massive loss of mitochondrial DNA. All three compounds increase reversion of a chromosomal point mutation (lys2-801(amber)), with Gelred being the most mutagenic and Redsafe the least. These dyes are all cationic and are probably taken by cells through non-selective cation channels. We could measure the glucose-energized transport of EtBr and Gelred inside the cells, while uptake of Redsafe was below our detection limit. We conclude that although all three compounds are toxic and mutagenic in the yeast system, Redsafe is the safest for yeast, probably because of very limited uptake by these cells. Copyright (c) 2015 John Wiley & Sons, Ltd. | es_ES |
dc.description.sponsorship | This work was funded by the Ministerio de Ciencia e Innovacion, Madrid, Spain (Grant No. BFU2011-22526). | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation.ispartof | Yeast | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Ethidium bromide | es_ES |
dc.subject | Redsafe | es_ES |
dc.subject | Gelred | es_ES |
dc.subject | Growth inhibition | es_ES |
dc.subject | petite induction | es_ES |
dc.subject | Mutation reversion | es_ES |
dc.subject | Dye uptake | es_ES |
dc.subject | Non-selective cation channels | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | Toxicity, mutagenicity and transport in Saccharomyces cerevisiae of three popular DNA intercalating fluorescent dyes | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1002/yea.3081 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BFU2011-22526/ES/NUEVOS MECANISMOS DE TRANSMISION DE SEÑALES DURANTE EL METABOLISMO DE GLUCOSA Y LA ACIDIFICACION INTRACELULAR: AMPLIANDO LAS FUNCIONES DE LA PROTEINA FOSFATASA 1 Y LA PROTEINA/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Sayas Montañana, EM.; García López, FJ.; Serrano Salom, R. (2015). Toxicity, mutagenicity and transport in Saccharomyces cerevisiae of three popular DNA intercalating fluorescent dyes. Yeast. 32(9):595-606. https://doi.org/10.1002/yea.3081 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://dx.doi.org/10.1002/yea.3081 | es_ES |
dc.description.upvformatpinicio | 595 | es_ES |
dc.description.upvformatpfin | 606 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 32 | es_ES |
dc.description.issue | 9 | es_ES |
dc.relation.senia | 308433 | es_ES |
dc.identifier.eissn | 1097-0061 | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |