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Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products

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Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products

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dc.contributor.author Tortajada-Genaro, Luis Antonio es_ES
dc.contributor.author Rodrigo, Alejandro es_ES
dc.contributor.author Hevia, Elizabeth es_ES
dc.contributor.author Mena, Salvador es_ES
dc.contributor.author Niñoles Rodenes, Regina
dc.contributor.author Maquieira Catala, Ángel
dc.date.accessioned 2016-07-01T10:33:05Z
dc.date.available 2016-07-01T10:33:05Z
dc.date.issued 2015-09
dc.identifier.issn 1618-2642
dc.identifier.uri http://hdl.handle.net/10251/66917
dc.description.abstract [EN] Highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food chain can be tailor-designed. Here, a novel biosensor is presented for the generic detection of Salmonella and Campylobacter and the discrimination between their most prevalent serovars (Salmonella Enteritidis, Salmonella Typhimurium) and species (Campylobacter jejuni, Campylobacter coli), respectively. The method is based on DNA microarray developed on a standard digital versatile disc (DVD) as support for a hybridization assay and a DVD driver as scanner. This approach was found to be highly sensitive (detection limit down to 0.2 pg of genomic DNA), reproducible (relative standard deviation 4-19 %), and high working capacity (20 samples per disc). The inclusivity and exclusivity assays indicated that designed oligonucleotides (primers and probes) were able to discriminate targeted pathogens from other Salmonella serovars, Campylobacter species, or common food-borne pathogens potentially present in the indigenous microflora. One hundred isolates from meat samples, collected in a poultry factory, were analyzed by the DVD microarraying and fluorescent real-time PCR. An excellent correlation was observed for both generic and specific detection (relative sensitivity 93-99 % and relative specificity 93-100 %). Therefore, the developed assay has been shown to be a reliable tool to be used in routine food safety analysis, especially in settings with limited infrastructure due to the excellent efficiency-cost ratio of compact disc technology. Graphical Abstract DNA microarray performed by DVD technology for pathogen genotyping. es_ES
dc.description.sponsorship This work has been supported by the Spanish Ministry of Economy and Competitiveness projects:INNPACTO (Ref. IPT-2011-1011-31000) and FEDER-CTQ2013-45875-R). Contribution of grant PROMETEO II/2014/040 (Generalitat Valenciana) was highly acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Spanish Ministry of Economy and Competitiveness FEDER-CTQ2013-45875-R es_ES
dc.relation Spanish Ministry of Economy and Competitiveness IPT-2011-1011-31000 es_ES
dc.relation Generalitat Valenciana PROMETEO II/2014/040 es_ES
dc.relation.ispartof Analytical and Bioanalytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Genotyping es_ES
dc.subject Food-borne pathogens es_ES
dc.subject DVD es_ES
dc.subject Borne pathogens es_ES
dc.subject DNA assay es_ES
dc.subject Compact disc es_ES
dc.subject Microarray es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00216-015-8890-0
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Tortajada-Genaro, LA.; Rodrigo, A.; Hevia, E.; Mena, S.; Niñoles Rodenes, R.; Maquieira Catala, A. (2015). Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products. Analytical and Bioanalytical Chemistry. 407(24):7285-7294. doi:10.1007/s00216-015-8890-0 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1007/s00216-015-8890-0 es_ES
dc.description.upvformatpinicio 7285 es_ES
dc.description.upvformatpfin 7294 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 407 es_ES
dc.description.issue 24 es_ES
dc.relation.senia 297367 es_ES
dc.identifier.eissn 1618-2650
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