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Channeled polymeric scaffolds with polypeptide gel filling for lengthwise guidance of neural cells

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Channeled polymeric scaffolds with polypeptide gel filling for lengthwise guidance of neural cells

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dc.contributor.author Conejero Garcia, Álvaro es_ES
dc.contributor.author Vilarino-Feltrer, G. es_ES
dc.contributor.author Martínez Ramos, Cristina es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.contributor.author Vallés Lluch, Ana es_ES
dc.date.accessioned 2016-07-05T07:33:36Z
dc.date.available 2016-07-05T07:33:36Z
dc.date.issued 2015-09
dc.identifier.issn 0014-3057
dc.identifier.uri http://hdl.handle.net/10251/67077
dc.description.abstract CNS damages are often irreversible since neurons of the central nervous system are unable to regenerate after an injury. As a new strategy within the nervous system tissue engineering, multifunctional systems based on two different biomaterials to support axonal guidance in damaged connective tracts have been developed herein. These systems are composed of a channeled scaffold made of ethyl acrylate and hydroxyethyl acrylate copolymer, P(EA-co-HEA), with parallel tubular micropores, combined with an injectable and in situ gelable self-assembling polypeptide (RAD16-I) as pores filler. The polymer scaffold is intended to provide a three-dimensional context for axon growth; subsequently, its morphology and physicochemical parameters have been determined by scanning electron microscopy, density measurements and compression tests. Besides, the hydrogel acts as a cell-friendly nanoenvironment while it creates a gradient of bioactive molecules (nerve growth factor, NGF) along the scaffolds channels; the chemotactic effect of NGF has been evaluated by a quantitative ELISA assay. These multifunctional systems have shown ability to keep circulating NGF, as well as proper short-term in vitro biological response with glial cells and neural progenitors. es_ES
dc.description.sponsorship The authors acknowledge funding through the Spanish Ministerio de Ciencia e Innovacion (MAT2011-28791-C03-02 and -03). Dr. J.M. Garcia Verdugo (Department of Comparative Neurobiology, Cavanilles Institute of Biodiversity and Evolutive Biology, Universitat de Valencia) is thanked for kindly providing the cells employed in this work. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Spanish Ministerio de Ciencia e Innovacion MAT2011-28791-C03-02 MAT2011-28791-C03-03 es_ES
dc.relation.ispartof European Polymer Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Scaffold es_ES
dc.subject NGF es_ES
dc.subject RAD16-I es_ES
dc.subject Neural conduct es_ES
dc.subject Neuroglia es_ES
dc.subject Axonal growth es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Channeled polymeric scaffolds with polypeptide gel filling for lengthwise guidance of neural cells es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.eurpolymj.2015.07.033
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.description.bibliographicCitation Conejero García, Á.; Vilarino-Feltrer, G.; Martínez Ramos, C.; Monleón Pradas, M.; Vallés Lluch, A. (2015). Channeled polymeric scaffolds with polypeptide gel filling for lengthwise guidance of neural cells. European Polymer Journal. 70:331-341. doi:10.1016/j.eurpolymj.2015.07.033 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.eurpolymj.2015.07.033 es_ES
dc.description.upvformatpinicio 331 es_ES
dc.description.upvformatpfin 341 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 70 es_ES
dc.relation.senia 292898 es_ES
dc.identifier.eissn 1873-1945


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