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Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species.

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Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species.

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dc.contributor.author Ben Salem, I. es_ES
dc.contributor.author Correia, K. C. es_ES
dc.contributor.author Boughalleb, N. es_ES
dc.contributor.author Michereff, Sami Jorge es_ES
dc.contributor.author León Santana, Maela es_ES
dc.contributor.author Abad Campos, Paloma es_ES
dc.contributor.author García Jiménez, José es_ES
dc.contributor.author Armengol Fortí, Josep es_ES
dc.date.accessioned 2016-07-07T13:20:24Z
dc.date.available 2016-07-07T13:20:24Z
dc.date.issued 2013-06
dc.identifier.issn 0191-2917
dc.identifier.uri http://hdl.handle.net/10251/67329
dc.description.abstract [EN] Three Monosporascus eutypoides–like isolates recovered from cucurbit plants with symptoms of Monosporascus root rot and vine decline in Tunisia were compared to 28 isolates of M. cannonballus from 12 countries for phenotypic, genomic, and pathogenicity characteristics. Morphologically, M. cannonballus and M. eutypoides–like cultures were similar, each producing fertile perithecia in culture containing globose, smooth, dark brown to black ascospores. Nevertheless, all M. cannonballus isolates had one ascospore per ascus, while M. eutypoides–like isolates had mainly two to three ascospores per ascus (rarely one). The employment of the internal transcribed spacer (ITS) of nuclear ribosomal DNA, the elongation factor 1-α (EF-1α), and the β-tubulin (β-tub) gene sequence diversity analyses and the resulting phylogenies identified a level of polymorphism that enabled separation of M. cannonballus and M. eutypoides–like isolates. All isolates of M. cannonballus had identical EF-1α and β-tub sequences irrespective of very diverse geographic origins, which were different from the EF-1α and β-tub sequences of the M. eutypoides–like isolates (96 and 97% similarity, respectively). Similar results were obtained for the ITS region of rDNA. In addition, of three M. eutypoides–like isolates tested for pathogenicity, all three were pathogenic on watermelon, two were pathogenic on muskmelon, but only one was pathogenic on cucumber. The results demonstrate that the M. eutypoides–like isolates belong to the species M. eutypoides, and that M. cannonballus and M. eutypoides are distinct species. es_ES
dc.description.sponsorship We thank Z. Banihashemi (College of Agriculture, Shiraz University, Shiraz, Iran), B. D. Bruton (USDA, Lane, OK, USA), A. Infantino (Istituto Sperimentale per la Patologia Vegetale, Rome, Italy), G. Quilosi (Facolta di Agraria, Universita degli Studi della Tuscia, Viterbo, Italy), and A. Sarpeleh (Plant Protection Research Institute, Tehran, Iran) for providing strains of Monosporascus, without which this study would not have been possible. This research was supported financially by the Projects A/3878/05, A/5352/06, and A/017593/08 (Programa de Cooperacion Cientifica e Investigacion Inter-Universitaria entre Espana y Tunez, PCI-Mediterraneo, Ministerio de Asuntos Exteriores y Cooperacion), and by CAPES (Project 203/2009 - International Cooperation CAPES-Brazil/DGU-Spain). We are thankful to CAPES (Brazil) for the research fellowships granted to K. C. Correia and S. J. Michereff.
dc.language Inglés es_ES
dc.publisher American Phytopathological Society es_ES
dc.relation MAEC/A/3878/05 es_ES
dc.relation CAPES/203/2009 es_ES
dc.relation MAEC/A/5352/06 es_ES
dc.relation MAEC/A/017593/08
dc.relation.ispartof Plant Disease es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Sequence-analysis es_ES
dc.subject Cantaloupe roots es_ES
dc.subject IMI descriptions es_ES
dc.subject Fungi es_ES
dc.subject RDNA es_ES
dc.subject Ascomycete es_ES
dc.subject Watermelon es_ES
dc.subject Ascopores es_ES
dc.subject Position es_ES
dc.subject Disease es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1094/ PDIS-05-12-0464-RE
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.description.bibliographicCitation Ben Salem, I.; Correia, KC.; Boughalleb, N.; Michereff, SJ.; León Santana, M.; Abad Campos, P.; García Jiménez, J.... (2013). Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species. Plant Disease. 97(6):737-743. doi:10.1094/ PDIS-05-12-0464-RE es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1094/PDIS-05-12-0464-RE es_ES
dc.description.upvformatpinicio 737 es_ES
dc.description.upvformatpfin 743 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 97 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 245598 es_ES
dc.contributor.funder Ministerio de Asuntos Exteriores y Cooperación (MAEC)
dc.contributor.funder Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES)


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